ID TXDP2_PARLU STANDARD; PRT; 37 AA. AC P83257; DT 28-NOV-2002, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2002, sequence version 1. DT 17-OCT-2006, entry version 25. DE Delta-palutoxin IT2 (Delta-paluIT2). OS Paracoelotes luctuosus (Spider). OC Eukaryota; Metazoa; Arthropoda; Chelicerata; Arachnida; Araneae; OC Araneomorphae; Entelegynae; Amaurobiidae; Paracoelotes. OX NCBI_TaxID=185217; RN [1] RP PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY. RC TISSUE=Venom; RX MEDLINE=20428467; PubMed=10971590; RA Corzo G., Escoubas P., Stankiewicz M., Pelhate M., Kristensen C.P., RA Nakajima T.; RT "Isolation, synthesis and pharmacological characterization of delta- RT palutoxins IT, novel insecticidal toxins from the spider Paracoelotes RT luctuosus (Amaurobiidae)."; RL Eur. J. Biochem. 267:5783-5795(2000). RN [2] RP STRUCTURE BY NMR. RX PubMed=15726637; DOI=10.1002/prot.20424; RA Ferrat G., Bosmans F., Tytgat J., Pimentel C., Chagot B., Gilles N., RA Nakajima T., Darbon H., Corzo G.; RT "Solution structure of two insect-specific spider toxins and their RT pharmacological interaction with the insect voltage-gated Na+ RT channel."; RL Proteins 59:368-379(2005). CC -!- FUNCTION: Potent activity against S.litura larvae. CC -!- FUNCTION: Binds to sodium channels and inhibits the inactivation CC of the activated channels. This toxin is active only on insects CC (By similarity). CC -!- SUBCELLULAR LOCATION: Secreted protein. CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. CC -!- MASS SPECTROMETRY: MW=4114.6; METHOD=MALDI; RANGE=1-37; CC NOTE=Ref.1. CC -!- SIMILARITY: Belongs to the mu-agatoxin family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR PDB; 1V91; NMR; A=1-37. DR GO; GO:0005576; C:extracellular region; NAS. DR GO; GO:0019871; F:sodium channel inhibitor activity; IDA. DR GO; GO:0009405; P:pathogenesis; IDA. DR InterPro; IPR009243; Toxin_7. DR Pfam; PF05980; Toxin_7; 1. DR PROSITE; PS60015; MU_AGATOXIN; 1. KW 3D-structure; Amidation; Direct protein sequencing; KW Ionic channel inhibitor; Neurotoxin; Sodium channel inhibitor; Toxin. FT PEPTIDE 1 37 Delta-palutoxin IT2. FT /FTId=PRO_0000044962. FT MOD_RES 37 37 Serine amide. FT DISULFID 2 18 FT DISULFID 9 23 FT DISULFID 17 33 FT DISULFID 25 31 FT STRAND 5 8 FT TURN 11 13 FT STRAND 22 24 FT STRAND 27 30 FT STRAND 32 34 SQ SEQUENCE 37 AA; 4124 MW; 861E12C2EB547716 CRC64; ACVGDGQRCA SWSGPYCCDG YYCSCRSMPY CRCRNNS //