ID IMPI_GALME STANDARD; PRT; 170 AA. AC P82176; DT 16-OCT-2001 (Rel. 40, Created) DT 10-OCT-2003 (Rel. 42, Last sequence update) DT 05-JUL-2004 (Rel. 44, Last annotation update) DE Inducible metalloproteinase inhibitor protein precursor [Contains: DE IMPI alpha]. GN Name=IMPI; OS Galleria mellonella (Wax moth). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea; OC Pyralidae; Galleriinae; Galleria. OX NCBI_TaxID=7137; RN [1] RP NUCLEOTIDE SEQUENCE. RA Weise C., Bender O., Kopacek P., Hucho F.; RT "Hemolymph proteins of the greater wax moth, Galleria mellonella."; RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE OF 57-80, CHARACTERIZATION, AND GLYCOSYLATION. RC TISSUE=Larval hemolymph; RX MEDLINE=98409271; PubMed=9738891; RA Wedde M., Weise C., Kopacek P., Franke P., Vilcinskas A.; RT "Purification and characterization of an inducible metalloprotease RT inhibitor from the hemolymph of greater wax moth larvae, Galleria RT mellonella."; RL Eur. J. Biochem. 255:535-543(1998). RN [3] RP SEQUENCE REVISION TO 63, AND MASS SPECTROMETRY. RA Weise C.; RL Submitted (JUL-2003) to Swiss-Prot. CC -!- FUNCTION: Inhibits thermolysin and the metalloprotease of CC B.polymyxa. No activity on trypsin or cysteinprotease papain. CC -!- INDUCTION: During humoral immune response. By lipopolysaccharide CC (LPS). CC -!- PTM: Cleaved (Probable). CC -!- PTM: Five disulfide bonds are present. When artificially cleaved CC by thermolysin between Asn-56 and Ile-57, the two obtained chains CC (called heavy and light chains) remain linked. CC -!- MASS SPECTROMETRY: MW=8360; METHOD=MALDI; RANGE=20-88; NOTE=Ref.3. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AJ577749; CAE12200.1; -. DR InterPro; IPR002919; Cysrich_TIL. DR InterPro; IPR006209; EGF_like. DR Pfam; PF01826; TIL; 1. DR PROSITE; PS01186; EGF_2; UNKNOWN_2. KW Glycoprotein; Metalloprotease inhibitor; Signal. FT SIGNAL 1 19 Potential. FT CHAIN 20 170 Inducible metalloproteinase inhibitor FT protein. FT CHAIN 20 88 IMPI alpha. FT SITE 88 89 Cleavage (Probable). FT CARBOHYD 48 48 N-linked (GlcNAc...). FT CARBOHYD 149 149 N-linked (GlcNAc...) (Potential). SQ SEQUENCE 170 AA; 18759 MW; 1A5B272846AD129A CRC64; MKCLLYLCLW CYCVLVSSSI VLICNGGHEY YECGGACDNV CADLHIQNKT NCPIINIRCN DKCYCEDGYA RDVNGKCIPI KDCPKIRSRR SIGIPVDKKC CTGPNEHYDE EKVSCPPETC ISLVAKFSCI DSPPPSPGCS CNSGYLRLNL TSPCIPICDC PQMQHSPDCQ //