ID OR33A_DROME Reviewed; 378 AA. AC P81914; DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1999, sequence version 1. DT 20-JAN-2009, entry version 66. DE RecName: Full=Odorant receptor 33a; GN Name=Or33a; Synonyms=AN3, DOR33B.1, dor73, Or33B.1; ORFNames=CG16960; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=99389723; PubMed=10458908; DOI=10.1006/geno.1999.5894; RA Gao Q., Chess A.; RT "Identification of candidate Drosophila olfactory receptors from RT genomic DNA sequence."; RL Genomics 60:31-39(1999). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., RA Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [3] RP GENOME REANNOTATION. RX MEDLINE=22426069; PubMed=12537572; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] RP IDENTIFICATION, AND TISSUE SPECIFICITY. RX MEDLINE=99166868; PubMed=10069338; DOI=10.1016/S0896-6273(00)81093-4; RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.; RT "A novel family of divergent seven-transmembrane proteins: candidate RT odorant receptors in Drosophila."; RL Neuron 22:327-338(1999). RN [5] RP IDENTIFICATION, AND TISSUE SPECIFICITY. RC STRAIN=Oregon-R; TISSUE=Antenna; RX MEDLINE=99189757; PubMed=10089887; DOI=10.1016/S0092-8674(00)80582-6; RA Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R.; RT "A spatial map of olfactory receptor expression in the Drosophila RT antenna."; RL Cell 96:725-736(1999). CC -!- FUNCTION: Complexes with Or83b to form odorant-sensing units, CC providing sensitive and prolonged odorant signaling and calcium CC permeability. They are necessary and sufficient to promote CC functional reconstitution of odor-evoked signaling in sensory CC neurons that normally respond only to carbon dioxide (By CC similarity). CC -!- SUBUNIT: Interacts with Or83b. Complexes exist early in the CC endomembrane system in olfactory sensory neurons (OSNs), coupling CC these complexes to the conserved ciliary trafficking pathway (By CC similarity). CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein CC (By similarity). CC -!- TISSUE SPECIFICITY: Expressed in 1-2 cells on the distal edge of CC the antenna but not the maxillary palp. CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of CC the odorant receptors appears to be an insect-specific solution CC for odor recognition, making the OR/Or83b complex an attractive CC target for the development of highly selective insect repellents CC to disrupt olfactory-mediated host-seeking behaviors of insect CC disease vectors. Odor-evoked OR currents are independent of known CC G-protein-coupled second messenger pathways. CC -!- SIMILARITY: Belongs to the odorant-gated ionic channel family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014134; AAF53131.1; -; Genomic_DNA. DR RefSeq; NP_523553.1; -. DR UniGene; Dm.26795; -. DR DIP; DIP:22508N; -. DR IntAct; P81914; 2. DR Ensembl; CG16960; Drosophila melanogaster. DR GeneID; 34601; -. DR KEGG; dme:Dmel_CG16960; -. DR NMPDR; fig|7227.3.peg.2047; -. DR FlyBase; FBgn0026392; Or33a. DR HOGENOM; P81914; -. DR BioCyc; DMEL-XXX-02:DMEL-XXX-02-008600-MON; -. DR NextBio; 789255; -. DR ArrayExpress; P81914; -. DR GermOnline; CG16960; Drosophila melanogaster. DR GO; GO:0016021; C:integral to membrane; NAS:UniProtKB. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW. DR GO; GO:0005549; F:odorant binding; IEA:InterPro. DR GO; GO:0004984; F:olfactory receptor activity; NAS:UniProtKB. DR GO; GO:0007186; P:G-protein coupled receptor protein signalin...; NAS:UniProtKB. DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW. DR GO; GO:0007608; P:sensory perception of smell; NAS:UniProtKB. DR InterPro; IPR004117; 7tm6_olfct_rcpt. DR PANTHER; PTHR21137; 7tm6_olfct_rcpt; 1. DR Pfam; PF02949; 7tm_6; 1. PE 2: Evidence at transcript level; KW Cell membrane; Complete proteome; G-protein coupled receptor; KW Membrane; Olfaction; Receptor; Sensory transduction; Transducer; KW Transmembrane. FT CHAIN 1 378 Odorant receptor 33a. FT /FTId=PRO_0000174238. FT TOPO_DOM 1 33 Cytoplasmic (Potential). FT TRANSMEM 34 54 1 (Potential). FT TOPO_DOM 55 62 Extracellular (Potential). FT TRANSMEM 63 83 2 (Potential). FT TOPO_DOM 84 127 Cytoplasmic (Potential). FT TRANSMEM 128 148 3 (Potential). FT TOPO_DOM 149 163 Extracellular (Potential). FT TRANSMEM 164 184 4 (Potential). FT TOPO_DOM 185 254 Cytoplasmic (Potential). FT TRANSMEM 255 275 5 (Potential). FT TOPO_DOM 276 285 Extracellular (Potential). FT TRANSMEM 286 306 6 (Potential). FT TOPO_DOM 307 355 Cytoplasmic (Potential). FT TRANSMEM 356 376 7 (Potential). FT TOPO_DOM 377 378 Extracellular (Potential). SQ SEQUENCE 378 AA; 43892 MW; D5BE000865EBCA21 CRC64; MDSRRKVRSE NLYKTYWLYW RLLGVEGDYP FRRLVDFTIT SFITILFPVH LILGMYKKPQ IQVFRSLHFT SECLFCSYKF FCFRWKLKEI KTIEGLLQDL DSRVESEEER NYFNQNPSRV ARMLSKSYLV AAISAIITAT VAGLFSTGRN LMYLGWFPYD FQATAAIYWI SFSYQAIGSS LLILENLAND SYPPITFCVV SGHVRLLIMR LSRIGHDVKL SSSENTRKLI EGIQDHRKLM KIIRLLRSTL HLSQLGQFLS SGINISITLI NILFFAENNF AMLYYAVFFA AMLIELFPSC YYGILMTMEF DKLPYAIFSS NWLKMDKRYN RSLIILMQLT LVPVNIKAGG IVGIDMSAFF ATVRMAYSFY TLALSFRV //