ID OR33A_DROME Reviewed; 378 AA. AC P81914; DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1999, sequence version 1. DT 25-MAY-2022, entry version 150. DE RecName: Full=Odorant receptor 33a; GN Name=Or33a; Synonyms=AN3, DOR33B.1, dor73, Or33B.1; ORFNames=CG16960; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX PubMed=10458908; DOI=10.1006/geno.1999.5894; RA Gao Q., Chess A.; RT "Identification of candidate Drosophila olfactory receptors from genomic RT DNA sequence."; RL Genomics 60:31-39(1999). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., RA Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [3] RP GENOME REANNOTATION. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] RP IDENTIFICATION, AND TISSUE SPECIFICITY. RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4; RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.; RT "A novel family of divergent seven-transmembrane proteins: candidate RT odorant receptors in Drosophila."; RL Neuron 22:327-338(1999). RN [5] RP IDENTIFICATION, AND TISSUE SPECIFICITY. RC STRAIN=Oregon-R; TISSUE=Antenna; RX PubMed=10089887; DOI=10.1016/s0092-8674(00)80582-6; RA Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R.; RT "A spatial map of olfactory receptor expression in the Drosophila RT antenna."; RL Cell 96:725-736(1999). RN [6] RP TISSUE SPECIFICITY. RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0; RA Vosshall L.B., Wong A.M., Axel R.; RT "An olfactory sensory map in the fly brain."; RL Cell 102:147-159(2000). CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance CC behavior, depending on its substrates. The odorant receptor repertoire CC encodes a large collection of odor stimuli that vary widely in CC identity, intensity, and duration. May form a complex with Orco to form CC odorant-sensing units, providing sensitive and prolonged odorant CC signaling and calcium permeability (By similarity). {ECO:0000250}. CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane CC system in olfactory sensory neurons (OSNs), coupling these complexes to CC the conserved ciliary trafficking pathway (By similarity). CC {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane CC protein {ECO:0000250}. CC -!- TISSUE SPECIFICITY: Expressed in 1-2 cells on the distal edge of the CC antenna but not the maxillary palp. {ECO:0000269|PubMed:10069338, CC ECO:0000269|PubMed:10089887, ECO:0000269|PubMed:10943836}. CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the CC odorant receptors appears to be an insect-specific solution for odor CC recognition, making the OR/Orco complex an attractive target for the CC development of highly selective insect repellents to disrupt olfactory- CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked CC OR currents are independent of known G-protein-coupled second messenger CC pathways. CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE014134; AAF53131.1; -; Genomic_DNA. DR RefSeq; NP_523553.1; NM_078829.1. DR AlphaFoldDB; P81914; -. DR SMR; P81914; -. DR BioGRID; 60654; 2. DR DIP; DIP-22508N; -. DR STRING; 7227.FBpp0079862; -. DR PaxDb; P81914; -. DR EnsemblMetazoa; FBtr0080278; FBpp0079862; FBgn0026392. DR GeneID; 34601; -. DR KEGG; dme:Dmel_CG16960; -. DR CTD; 34601; -. DR FlyBase; FBgn0026392; Or33a. DR VEuPathDB; VectorBase:FBgn0026392; -. DR eggNOG; ENOG502TBPZ; Eukaryota. DR GeneTree; ENSGT00540000073151; -. DR HOGENOM; CLU_033399_8_1_1; -. DR InParanoid; P81914; -. DR OMA; MANDSYP; -. DR OrthoDB; 745330at2759; -. DR PhylomeDB; P81914; -. DR BioGRID-ORCS; 34601; 0 hits in 1 CRISPR screen. DR GenomeRNAi; 34601; -. DR PRO; PR:P81914; -. DR Proteomes; UP000000803; Chromosome 2L. DR Bgee; FBgn0026392; Expressed in antenna and 3 other tissues. DR ExpressionAtlas; P81914; differential. DR Genevisible; P81914; DM. DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase. DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase. DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central. DR GO; GO:0005549; F:odorant binding; ISS:FlyBase. DR GO; GO:0004984; F:olfactory receptor activity; IDA:FlyBase. DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISM:FlyBase. DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IBA:GO_Central. DR GO; GO:0007608; P:sensory perception of smell; IEP:FlyBase. DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW. DR InterPro; IPR004117; 7tm6_olfct_rcpt. DR PANTHER; PTHR21137; PTHR21137; 1. DR Pfam; PF02949; 7tm_6; 1. PE 2: Evidence at transcript level; KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome; KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix. FT CHAIN 1..378 FT /note="Odorant receptor 33a" FT /id="PRO_0000174238" FT TOPO_DOM 1..33 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 34..54 FT /note="Helical; Name=1" FT /evidence="ECO:0000255" FT TOPO_DOM 55..62 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 63..83 FT /note="Helical; Name=2" FT /evidence="ECO:0000255" FT TOPO_DOM 84..127 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 128..148 FT /note="Helical; Name=3" FT /evidence="ECO:0000255" FT TOPO_DOM 149..163 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 164..184 FT /note="Helical; Name=4" FT /evidence="ECO:0000255" FT TOPO_DOM 185..254 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 255..275 FT /note="Helical; Name=5" FT /evidence="ECO:0000255" FT TOPO_DOM 276..285 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 286..306 FT /note="Helical; Name=6" FT /evidence="ECO:0000255" FT TOPO_DOM 307..355 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 356..376 FT /note="Helical; Name=7" FT /evidence="ECO:0000255" FT TOPO_DOM 377..378 FT /note="Extracellular" FT /evidence="ECO:0000255" SQ SEQUENCE 378 AA; 43892 MW; D5BE000865EBCA21 CRC64; MDSRRKVRSE NLYKTYWLYW RLLGVEGDYP FRRLVDFTIT SFITILFPVH LILGMYKKPQ IQVFRSLHFT SECLFCSYKF FCFRWKLKEI KTIEGLLQDL DSRVESEEER NYFNQNPSRV ARMLSKSYLV AAISAIITAT VAGLFSTGRN LMYLGWFPYD FQATAAIYWI SFSYQAIGSS LLILENLAND SYPPITFCVV SGHVRLLIMR LSRIGHDVKL SSSENTRKLI EGIQDHRKLM KIIRLLRSTL HLSQLGQFLS SGINISITLI NILFFAENNF AMLYYAVFFA AMLIELFPSC YYGILMTMEF DKLPYAIFSS NWLKMDKRYN RSLIILMQLT LVPVNIKAGG IVGIDMSAFF ATVRMAYSFY TLALSFRV //