ID TXM1_DENAN Reviewed; 66 AA. AC P81030; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1997, sequence version 1. DT 25-NOV-2008, entry version 40. DE RecName: Full=Muscarinic toxin 1; DE Short=MTx1; DE Short=MT1; OS Dendroaspis angusticeps (Eastern green mamba). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Lepidosauria; Squamata; Scleroglossa; Serpentes; Colubroidea; OC Elapidae; Elapinae; Dendroaspis. OX NCBI_TaxID=8618; RN [1] RP PROTEIN SEQUENCE. RX MEDLINE=94205875; PubMed=8154745; RA Karlsson E., Jolkkonen M., Satyapan N., Adem A., Kumlin E., RA Hellman U., Wernstedt C.; RT "Protein toxins that bind to muscarinic acetylcholine receptors."; RL Ann. N. Y. Acad. Sci. 710:153-161(1994). RN [2] RP PROTEIN SEQUENCE. RX MEDLINE=96018124; PubMed=7570626; DOI=10.1016/0041-0101(94)00102-E; RA Jolkkonen M., Adem A., Hellman U., Wernstedt C., Karlsson E.; RT "A snake toxin against muscarinic acetylcholine receptors: amino acid RT sequence, subtype specificity and effect on guinea-pig ileum."; RL Toxicon 33:399-410(1995). RN [3] RP FUNCTION. RX MEDLINE=95296971; PubMed=7778123; DOI=10.1016/0041-0101(94)00161-Z; RA Kornisiuk E., Jerusalinsky D., Cervenansky C., Harvey A.L.; RT "Binding of muscarinic toxins MTx1 and MTx2 from the venom of the RT green mamba Dendroaspis angusticeps to cloned human muscarinic RT cholinoceptors."; RL Toxicon 33:11-18(1995). CC -!- FUNCTION: Binds irreversibly to M1 muscarinic acetylcholine CC receptors. Has no effect on M2 and M4 receptors, but reveal a CC slightly weaker effect at M3 receptor. CC -!- SUBUNIT: Monomer (By similarity). CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Type A CC muscarinic toxin subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR HSSP; P18328; 1FF4. DR HOVERGEN; P81030; -. DR GO; GO:0005576; C:extracellular region; IEA:InterPro. DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW. DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW. DR GO; GO:0009405; P:pathogenesis; IEA:UniProtKB-KW. DR GO; GO:0007268; P:synaptic transmission; IEA:UniProtKB-KW. DR InterPro; IPR003572; Cytotoxin. DR InterPro; IPR003571; Snake_toxin. DR Pfam; PF00087; Toxin_1; 1. DR PRINTS; PR00282; CYTOTOXIN. DR ProDom; PD000206; Snake_toxin; 1. DR PROSITE; PS00272; SNAKE_TOXIN; 1. PE 1: Evidence at protein level; KW Acetylcholine receptor inhibitor; Direct protein sequencing; KW Neurotoxin; Postsynaptic neurotoxin; Secreted; Toxin. FT CHAIN 1 66 Muscarinic toxin 1. FT /FTId=PRO_0000093641. FT DISULFID 3 24 By similarity. FT DISULFID 17 42 By similarity. FT DISULFID 46 58 By similarity. FT DISULFID 59 64 By similarity. SQ SEQUENCE 66 AA; 7518 MW; EAAC074F4A00DD3E CRC64; LTCVTSKSIF GITTENCPDG QNLCFKKWYY IVPRYSDITW GCAATCPKPT NVRETIRCCE TDKCNE //