ID P79209 PRELIMINARY; PRT; 99 AA. AC P79209; DT 01-MAY-1997 (TrEMBLrel. 03, Created) DT 01-MAY-1997 (TrEMBLrel. 03, Last sequence update) DT 01-NOV-1999 (TrEMBLrel. 12, Last annotation update) DE NITRIC-OXIDE SYNTHASE, ENDOTHELIAL (EC 1.14.13.39) (EC-NOS) (NOS, TYPE DE III) (ENDOTHELIAL NOS) (ENOS) (CONSTITUTIVE NOS) (CNOS) (FRAGMENT). GN ENOS. OS Ovis aries (Sheep). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia; OC Eutheria; Cetartiodactyla; Ruminantia; Pecora; Bovoidea; Bovidae; OC Caprinae; Ovis. RN [1] RP SEQUENCE FROM N.A. RC TISSUE=ENDOTHELIAL CELLS; RA AGUAN K., WEINER C.P.; RT "Effect of hypoxia on the microvasculature of developing fetal brain RT of sheep: a studies on the expression pattern of constitutive forms of RT nitric oxide synthase."; RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: PRODUCES NITRIC OXIDE (NO) WHICH ACCOUNTS FOR THE CC BIOLOGIC ACTIVITY OF ENDOTHELIUM-DERIVED RELAXING FACTOR (EDRF) CC WHICH IS IMPORTANT IN REGULATION OF VASOMOTOR TONE AND BLOOD FLOW CC BY INHIBITING SMOOTH MUSCLE CONTRACTION AND PLATELET AGGREGATION CC (BY SIMILARITY). CC -!- CATALYTIC ACTIVITY: L-ARGININE + N NADPH + M O(2) = CITRULLINE + CC NITRIC OXIDE + N NADP(+). CC -!- COFACTOR: BINDS ONE MOLE EACH OF FAD AND FMN AND ALSO REQUIRES CC TETRAHYDROBIOPTERIN (BH4). CC -!- ENZYME REGULATION: STIMULATED BY CALCIUM/CALMODULIN (BY CC SIMILARITY). CC -!- SUBUNIT: HOMODIMER (BY SIMILARITY). CC -!- SIMILARITY: STRONG, TO OTHER NOS ISOZYMES. ALSO TO CYTOCHROME P- CC 450 REDUCTASE. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U76738; AAB40705.1; -. DR HSSP; P29477; 1NOC. KW Oxidoreductase; NADP; FAD; FMN; Calmodulin-binding; Myristylation; KW Lipoprotein; Palmitate; Heme; Calcium-binding; Multigene family. FT NON_TER 1 1 FT NON_TER 99 99 SQ SEQUENCE 99 AA; 11034 MW; 43EE56E8 CRC32; VAVCMDLDTR TTSSLWKDKA AVEINLAVLH SFQLAKVTIV DHHAATVSFM KHLENEQKAR GGCPADWAWI VPPISGSLTP VFHQEMVNYV LSPAFRYQP //