ID NOS3_SHEEP STANDARD; PRT; 99 AA. AC P79209; DT 30-MAY-2000 (Rel. 39, Created) DT 30-MAY-2000 (Rel. 39, Last sequence update) DT 30-MAY-2000 (Rel. 39, Last annotation update) DE Nitric-oxide synthase, endothelial (EC 1.14.13.39) (EC-NOS) (NOS, type DE III) (NOSIII) (Endothelial NOS) (ENOS) (Constitutive NOS) (CNOS) DE (Fragment). GN NOS3 OR ENOS. OS Ovis aries (Sheep). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Cetartiodactyla; Ruminantia; Pecora; Bovoidea; OC Bovidae; Caprinae; Ovis. OX NCBI_TaxID=9940; RN [1] RP SEQUENCE FROM N.A. RC TISSUE=Endothelial cells; RA Aguan K., Weiner C.P.; RT "Effect of hypoxia on the microvasculature of developing fetal RT brain of sheep: a studies on the expression pattern of RT constitutive forms of nitric oxide synthase."; RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: PRODUCES NITRIC OXIDE (NO) WHICH IS IMPLICATED IN CC VASCULAR SMOOTH MUSCLE RELAXATION THROUGH A CGMP-MEDIATED SIGNAL CC TRANSDUCTION PATHWAY. NO MEDIATES VASCULAR ENDOTHELIAL GROWTH CC FACTOR (VEGF)-INDUCED ANGIOGENESIS IN CORONARY VESSELS AND CC PROMOTES BLOOD CLOTTING THROUGH THE ACTIVATION OF PLATELETS (BY CC SIMILARITY). CC -!- CATALYTIC ACTIVITY: L-arginine + N NADPH + M O(2) = citrulline + CC nitric oxide + N NADP(+). CC -!- COFACTOR: HEME. BINDS ONE MOLE EACH OF FAD AND FMN. ALSO REQUIRES CC TETRAHYDROBIOPTERIN (BH4) WHICH MAY STABILIZE THE DIMERIC FORM OF CC THE ENZYME (BY SIMILARITY). CC -!- ENZYME REGULATION: STIMULATED BY CALCIUM/CALMODULIN (BY CC SIMILARITY). CC -!- SUBUNIT: HOMODIMER (BY SIMILARITY). CC -!- SIMILARITY: BELONGS TO THE NOS FAMILY. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U76738; AAB40705.1; -. DR HSSP; P29473; 1D0C. DR InterPro; IPR004030; NO_synthase. DR Pfam; PF02898; NO_synthase; 1. DR PROSITE; PS60001; NOS; PARTIAL. KW Oxidoreductase; NADP; FAD; FMN; Calmodulin-binding; Calcium-binding; KW Heme; Multigene family. FT NON_TER 1 1 FT NON_TER 99 99 SQ SEQUENCE 99 AA; 11034 MW; 82C3C765557031DA CRC64; VAVCMDLDTR TTSSLWKDKA AVEINLAVLH SFQLAKVTIV DHHAATVSFM KHLENEQKAR GGCPADWAWI VPPISGSLTP VFHQEMVNYV LSPAFRYQP //