ID VTC4_SCHPO Reviewed; 721 AA. AC P78810; Q1L850; DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2000, sequence version 2. DT 09-DEC-2015, entry version 88. DE RecName: Full=Vacuolar transporter chaperone 4; GN Name=vtc4; ORFNames=SPCC1322.14c; OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; OC Schizosaccharomycetes; Schizosaccharomycetales; OC Schizosaccharomycetaceae; Schizosaccharomyces. OX NCBI_TaxID=284812; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=972 / ATCC 24843; RX PubMed=11859360; DOI=10.1038/nature724; RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., RA Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., RA James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., RA Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., RA Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., RA Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., RA Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., RA Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., RA Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., RA Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., RA Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.; RT "The genome sequence of Schizosaccharomyces pombe."; RL Nature 415:871-880(2002). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] OF 192-721. RC STRAIN=PR745; RX PubMed=9501991; DOI=10.1093/dnares/4.6.363; RA Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.; RT "Identification of open reading frames in Schizosaccharomyces pombe RT cDNAs."; RL DNA Res. 4:363-369(1997). RN [3] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495; THR-497; SER-501; RP THR-534 AND SER-546, AND IDENTIFICATION BY MASS SPECTROMETRY. RX PubMed=18257517; DOI=10.1021/pr7006335; RA Wilson-Grady J.T., Villen J., Gygi S.P.; RT "Phosphoproteome analysis of fission yeast."; RL J. Proteome Res. 7:1088-1097(2008). CC -!- FUNCTION: Component of the vacuolar transporter chaperone (VTC) CC complex, which plays a role in vacuolar membrane fusion. CC {ECO:0000250}. CC -!- SUBUNIT: The VTC complex is an integral membrane heterooligomer CC composed of at least vtc1, vtc2 and vtc4. {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane CC protein. CC -!- SIMILARITY: Belongs to the VTC4 family. {ECO:0000305}. CC -!- SIMILARITY: Contains 1 SPX domain. {ECO:0000255|PROSITE- CC ProRule:PRU00714}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CU329672; CAA22867.1; -; Genomic_DNA. DR EMBL; D89159; BAA13821.1; -; mRNA. DR PIR; T40945; T40945. DR PIR; T42520; T42520. DR RefSeq; NP_588142.1; NM_001023132.2. DR BioGrid; 275369; 1. DR MINT; MINT-4691301; -. DR MaxQB; P78810; -. DR EnsemblFungi; SPCC1322.14c.1; SPCC1322.14c.1:pep; SPCC1322.14c. DR GeneID; 2538788; -. DR KEGG; spo:SPCC1322.14c; -. DR EuPathDB; FungiDB:SPCC1322.14c; -. DR PomBase; SPCC1322.14c; vtc4. DR HOGENOM; HOG000209413; -. DR InParanoid; P78810; -. DR OMA; SKFIHGT; -. DR OrthoDB; EOG7W6WVG; -. DR PhylomeDB; P78810; -. DR NextBio; 20799970; -. DR PRO; PR:P78810; -. DR Proteomes; UP000002485; Chromosome III. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0005783; C:endoplasmic reticulum; IDA:PomBase. DR GO; GO:0000329; C:fungal-type vacuole membrane; ISO:PomBase. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0033254; C:vacuolar transporter chaperone complex; IBA:GO_Central. DR GO; GO:0016237; P:lysosomal microautophagy; ISO:PomBase. DR GO; GO:0006797; P:polyphosphate metabolic process; IBA:GO_Central. DR GO; GO:0007034; P:vacuolar transport; ISO:PomBase. DR GO; GO:0042144; P:vacuole fusion, non-autophagic; ISO:PomBase. DR InterPro; IPR003807; DUF202. DR InterPro; IPR004331; SPX_dom. DR InterPro; IPR018966; VTC_domain. DR Pfam; PF02656; DUF202; 1. DR Pfam; PF03105; SPX; 1. DR Pfam; PF09359; VTC; 1. DR PROSITE; PS51382; SPX; 1. PE 1: Evidence at protein level; KW Chaperone; Complete proteome; Membrane; Phosphoprotein; KW Reference proteome; Transmembrane; Transmembrane helix; Vacuole. FT CHAIN 1 721 Vacuolar transporter chaperone 4. FT /FTId=PRO_0000363396. FT TOPO_DOM 1 631 Cytoplasmic. {ECO:0000250}. FT TRANSMEM 632 652 Helical. {ECO:0000255}. FT TOPO_DOM 653 657 Vacuolar. {ECO:0000250}. FT TRANSMEM 658 678 Helical. {ECO:0000255}. FT TOPO_DOM 679 697 Cytoplasmic. {ECO:0000250}. FT TRANSMEM 698 718 Helical. {ECO:0000255}. FT TOPO_DOM 719 721 Vacuolar. {ECO:0000250}. FT DOMAIN 1 146 SPX. {ECO:0000255|PROSITE- FT ProRule:PRU00714}. FT MOD_RES 495 495 Phosphoserine. FT {ECO:0000269|PubMed:18257517}. FT MOD_RES 497 497 Phosphothreonine. FT {ECO:0000269|PubMed:18257517}. FT MOD_RES 501 501 Phosphoserine. FT {ECO:0000269|PubMed:18257517}. FT MOD_RES 534 534 Phosphothreonine. FT {ECO:0000269|PubMed:18257517}. FT MOD_RES 546 546 Phosphoserine. FT {ECO:0000269|PubMed:18257517}. FT CONFLICT 580 580 K -> R (in Ref. 2; BAA13821). FT {ECO:0000305}. FT CONFLICT 714 714 N -> Y (in Ref. 2; BAA13821). FT {ECO:0000305}. SQ SEQUENCE 721 AA; 83635 MW; DF4471E8FC0A6BCF CRC64; MKFGQLLKET LMYEYKYSYV NYDKLKKEIK RRNDQGGWSE EDESDFVELL EKELDKVYSF QKNKSAEVME RIRFCEEQTD EVVRRLDSDN PPNENDFAIL ETELTDIMAT VHDLAKFSEL NYTAFYKIIK KHDKHTGWIL KPVFAARLNA KPFFKEQYDL LVVKLSKLYD FVRTRGSPIK GDSAAGGTQQ NFVRQTTKYW VHPNNVTELK IYILKHLPVL VFNPNKEFAR EDAAITSIYY DNDDLDFYLG RLEKREGAEA IRLRWYGNMD NNNIFVERKT HREDWTGEKS VKARFPLKEK YVNAFLRGDY TVEEAFAKMR KDGKKSLKEI ESLERLAKEV QYTVLSRGMK PYVRSFYERT AFQLPGDARV RISLDTNLSL IREDGPSRAG NNWRRMDIGI DYPFDQLPDE DIVRFPYAIL EVKLQTQFGQ DPPEWVNNLV NSHLVEAVPK FSKFIHGVST LFYDRVTLLP YWFPQMDIDI RKPATHTFIQ GRSQSGTHSS SVSANVLTDS ENTPIHADGD NYVDEESRIG SSSTRNDNST FQTSDSFQEL DERTNLLDIS KRKGRDSFVA ALNSRLKDIK DSFFLETVPK FEEPTEPTVI YEQKYVSPPG KRIYVPVRVE PKTYFALERT YLDYLRYSIL MGSIGITLFS FAKTRSGILG AASFTLVALF AIFYSTFLYL WRAVNIAKHN AVRYDDRFGP TAICVITFAA ISANVILNFN A //