ID VTC4_SCHPO Reviewed; 721 AA. AC P78810; Q1L850; DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-2000, sequence version 2. DT 25-MAY-2022, entry version 121. DE RecName: Full=Vacuolar transporter chaperone 4; GN Name=vtc4; ORFNames=SPCC1322.14c; OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; OC Schizosaccharomyces. OX NCBI_TaxID=284812; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=972 / ATCC 24843; RX PubMed=11859360; DOI=10.1038/nature724; RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., RA Nurse P.; RT "The genome sequence of Schizosaccharomyces pombe."; RL Nature 415:871-880(2002). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] OF 192-721. RC STRAIN=PR745; RX PubMed=9501991; DOI=10.1093/dnares/4.6.363; RA Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.; RT "Identification of open reading frames in Schizosaccharomyces pombe RT cDNAs."; RL DNA Res. 4:363-369(1997). RN [3] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-495; THR-497; SER-501; RP THR-534 AND SER-546, AND IDENTIFICATION BY MASS SPECTROMETRY. RX PubMed=18257517; DOI=10.1021/pr7006335; RA Wilson-Grady J.T., Villen J., Gygi S.P.; RT "Phosphoproteome analysis of fission yeast."; RL J. Proteome Res. 7:1088-1097(2008). CC -!- FUNCTION: Component of the vacuolar transporter chaperone (VTC) CC complex, which plays a role in vacuolar membrane fusion. {ECO:0000250}. CC -!- SUBUNIT: The VTC complex is an integral membrane heterooligomer CC composed of at least vtc1, vtc2 and vtc4. {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein. CC -!- SIMILARITY: Belongs to the VTC4 family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CU329672; CAA22867.1; -; Genomic_DNA. DR EMBL; D89159; BAA13821.1; -; mRNA. DR PIR; T40945; T40945. DR PIR; T42520; T42520. DR RefSeq; NP_588142.1; NM_001023132.2. DR AlphaFoldDB; P78810; -. DR SMR; P78810; -. DR BioGRID; 275369; 7. DR STRING; 4896.SPCC1322.14c.1; -. DR iPTMnet; P78810; -. DR MaxQB; P78810; -. DR PaxDb; P78810; -. DR PRIDE; P78810; -. DR EnsemblFungi; SPCC1322.14c.1; SPCC1322.14c.1:pep; SPCC1322.14c. DR GeneID; 2538788; -. DR KEGG; spo:SPCC1322.14c; -. DR PomBase; SPCC1322.14c; vtc4. DR VEuPathDB; FungiDB:SPCC1322.14c; -. DR eggNOG; KOG1161; Eukaryota. DR eggNOG; KOG4580; Eukaryota. DR HOGENOM; CLU_009308_0_0_1; -. DR InParanoid; P78810; -. DR OMA; AGGKQQN; -. DR PhylomeDB; P78810; -. DR PRO; PR:P78810; -. DR Proteomes; UP000002485; Chromosome III. DR GO; GO:0000329; C:fungal-type vacuole membrane; ISO:PomBase. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0033254; C:vacuolar transporter chaperone complex; ISO:PomBase. DR GO; GO:0006112; P:energy reserve metabolic process; IC:PomBase. DR GO; GO:0016237; P:lysosomal microautophagy; ISO:PomBase. DR GO; GO:0006799; P:polyphosphate biosynthetic process; ISO:PomBase. DR GO; GO:0006797; P:polyphosphate metabolic process; IBA:GO_Central. DR GO; GO:0007034; P:vacuolar transport; ISO:PomBase. DR GO; GO:0042144; P:vacuole fusion, non-autophagic; ISO:PomBase. DR Gene3D; 3.20.100.30; -; 1. DR InterPro; IPR003807; DUF202. DR InterPro; IPR004331; SPX_dom. DR InterPro; IPR018966; VTC_domain. DR InterPro; IPR042267; VTC_sf. DR Pfam; PF02656; DUF202; 1. DR Pfam; PF09359; VTC; 1. DR PROSITE; PS51382; SPX; 1. PE 1: Evidence at protein level; KW Chaperone; Membrane; Phosphoprotein; Reference proteome; Transmembrane; KW Transmembrane helix; Vacuole. FT CHAIN 1..721 FT /note="Vacuolar transporter chaperone 4" FT /id="PRO_0000363396" FT TOPO_DOM 1..631 FT /note="Cytoplasmic" FT /evidence="ECO:0000250" FT TRANSMEM 632..652 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 653..657 FT /note="Vacuolar" FT /evidence="ECO:0000250" FT TRANSMEM 658..678 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 679..697 FT /note="Cytoplasmic" FT /evidence="ECO:0000250" FT TRANSMEM 698..718 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 719..721 FT /note="Vacuolar" FT /evidence="ECO:0000250" FT DOMAIN 1..146 FT /note="SPX" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00714" FT REGION 490..547 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 490..515 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 526..547 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 495 FT /note="Phosphoserine" FT /evidence="ECO:0000269|PubMed:18257517" FT MOD_RES 497 FT /note="Phosphothreonine" FT /evidence="ECO:0000269|PubMed:18257517" FT MOD_RES 501 FT /note="Phosphoserine" FT /evidence="ECO:0000269|PubMed:18257517" FT MOD_RES 534 FT /note="Phosphothreonine" FT /evidence="ECO:0000269|PubMed:18257517" FT MOD_RES 546 FT /note="Phosphoserine" FT /evidence="ECO:0000269|PubMed:18257517" FT CONFLICT 580 FT /note="K -> R (in Ref. 2; BAA13821)" FT /evidence="ECO:0000305" FT CONFLICT 714 FT /note="N -> Y (in Ref. 2; BAA13821)" FT /evidence="ECO:0000305" SQ SEQUENCE 721 AA; 83635 MW; DF4471E8FC0A6BCF CRC64; MKFGQLLKET LMYEYKYSYV NYDKLKKEIK RRNDQGGWSE EDESDFVELL EKELDKVYSF QKNKSAEVME RIRFCEEQTD EVVRRLDSDN PPNENDFAIL ETELTDIMAT VHDLAKFSEL NYTAFYKIIK KHDKHTGWIL KPVFAARLNA KPFFKEQYDL LVVKLSKLYD FVRTRGSPIK GDSAAGGTQQ NFVRQTTKYW VHPNNVTELK IYILKHLPVL VFNPNKEFAR EDAAITSIYY DNDDLDFYLG RLEKREGAEA IRLRWYGNMD NNNIFVERKT HREDWTGEKS VKARFPLKEK YVNAFLRGDY TVEEAFAKMR KDGKKSLKEI ESLERLAKEV QYTVLSRGMK PYVRSFYERT AFQLPGDARV RISLDTNLSL IREDGPSRAG NNWRRMDIGI DYPFDQLPDE DIVRFPYAIL EVKLQTQFGQ DPPEWVNNLV NSHLVEAVPK FSKFIHGVST LFYDRVTLLP YWFPQMDIDI RKPATHTFIQ GRSQSGTHSS SVSANVLTDS ENTPIHADGD NYVDEESRIG SSSTRNDNST FQTSDSFQEL DERTNLLDIS KRKGRDSFVA ALNSRLKDIK DSFFLETVPK FEEPTEPTVI YEQKYVSPPG KRIYVPVRVE PKTYFALERT YLDYLRYSIL MGSIGITLFS FAKTRSGILG AASFTLVALF AIFYSTFLYL WRAVNIAKHN AVRYDDRFGP TAICVITFAA ISANVILNFN A //