ID THID_ECOLI Reviewed; 266 AA. AC P76422; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1997, sequence version 1. DT 05-FEB-2008, entry version 55. DE Phosphomethylpyrimidine kinase (EC 2.7.4.7) (HMP-phosphate kinase) DE (HMP-P kinase). GN Name=thiD; OrderedLocusNames=b2103, JW2090; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION. RC STRAIN=JC1552; RX MEDLINE=99173753; PubMed=10075431; RA Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.; RT "Cloning and characterization of the thiD/J gene of Escherichia coli RT encoding a thiamin-synthesizing bifunctional enzyme, RT hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."; RL Microbiology 145:495-501(1999). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX MEDLINE=97251358; PubMed=9097040; DOI=10.1093/dnares/3.6.379; RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., RA Yamamoto Y., Horiuchi T.; RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome RT corresponding to the 40.1-50.0 min region on the linkage map."; RL DNA Res. 3:379-392(1996). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). CC -!- FUNCTION: Catalyzes the phosphorylation of HMP-P to HMP-PP. CC -!- CATALYTIC ACTIVITY: ATP + 4-amino-2-methyl-5- CC phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5- CC diphosphomethylpyrimidine. CC -!- PATHWAY: Cofactor biosynthesis; thiamine pyrophosphate CC biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5- CC amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3. CC -!- PATHWAY: Cofactor biosynthesis; thiamine pyrophosphate CC biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5- CC amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3. CC -!- SIMILARITY: Belongs to the thiD family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D84200; BAA76742.1; -; Genomic_DNA. DR EMBL; U00096; AAC75164.1; -; Genomic_DNA. DR EMBL; AP009048; BAA15971.1; -; Genomic_DNA. DR PIR; F64977; F64977. DR RefSeq; AP_002701.1; -. DR RefSeq; NP_416606.1; -. DR HSSP; P55882; 1JXH. DR SMR; P76422; 1-266. DR DIP; DIP:6867N; -. DR IntAct; P76422; -. DR GeneID; 946459; -. DR GenomeReviews; U00096_GR; b2103. DR GenomeReviews; AP009048_GR; JW2090. DR KEGG; ecj:JW2090; -. DR KEGG; eco:b2103; -. DR EchoBASE; EB3821; -. DR EcoGene; EG14068; thiD. DR BioCyc; EcoCyc:HMP-P-KIN-MON; -. DR InterPro; IPR013749; HMP-P_kinase-1. DR InterPro; IPR004399; HMP-P_kinase-2. DR Pfam; PF08543; Phos_pyr_kin; 1. DR TIGRFAMs; TIGR00097; HMP-P_kinase; 1. PE 1: Evidence at protein level; KW Complete proteome; Kinase; Thiamine biosynthesis; Transferase. FT CHAIN 1 266 Phosphomethylpyrimidine kinase. FT /FTId=PRO_0000192018. SQ SEQUENCE 266 AA; 28634 MW; E555CEFE445B8F0A CRC64; MKRINALTIA GTDPSGGAGI QADLKTFSAL GAYGCSVITA LVAQNTRGVQ SVYRIEPDFV AAQLDSVFSD VRIDTTKIGM LAETDIVEAV AERLQRYQIQ NVVLDTVMLA KSGDPLLSPS AVATLRSRLL PQVSLITPNL PEAAALLDAP HARTEQEMLE QGRSLLAMGC GAVLMKGGHL DDEQSPDWLF TREGEQRFTA PRIMTKNTHG TGCTLSAALA ALRPRHTNWA DTVQEAKSWL SSALAQADTL EVGHGIGPVH HFHAWW //