ID THID_ECOLI STANDARD; PRT; 266 AA. AC P76422; DT 01-NOV-1997 (Rel. 35, Created) DT 01-NOV-1997 (Rel. 35, Last sequence update) DT 30-MAY-2000 (Rel. 39, Last annotation update) DE PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) (HMP-PHOSPHATE KINASE) DE (HMP-P KINASE). GN THID. OS Escherichia coli. OC Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae; OC Escherichia. RN [1] RP SEQUENCE FROM N.A. RC STRAIN=K12 / MG1655; RX MEDLINE; 97426617. RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=K12; RX MEDLINE; 97251358. RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., RA Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., RA Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., RA Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., RA Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., RA Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.; RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome RT corresponding to the 40.1-50.0 min region on the linkage map."; RL DNA Res. 3:379-392(1996). RN [3] RP SEQUENCE FROM N.A., AND CHARACTERIZATION. RC STRAIN=K12 / W3310; RX MEDLINE; 99173753. RA Mizote T., Tsuda M., Smith D.D.S., Nakayama H., Nakazawa T.; RT "Cloning and characterization of the thiD/J gene of Escherichia coli RT encoding a thiamin-synthesizing bifunctional enzyme, RT hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase."; RL Microbiology 145:495-501(1999). CC -!- FUNCTION: CATALYZES THE PHOSPHORYLATION OF HMP-P TO HMP-PP. CC -!- CATALYTIC ACTIVITY: ATP + 4-AMINO-2-METHYL-5- CC PHOSPHOMETHYLPYRIMIDINE = ADP + 4-AMINO-2-METHYL-5- CC DIPHOSPHOMETHYLPYRIMIDINE. CC -!- PATHWAY: THIAMINE BIOSYNTHESIS. CC -!- SIMILARITY: BELONGS TO THE THID FAMILY. CC -------------------------------------------------------------------------- CC This SWISS-PROT entry is copyright. It is produced through a collaboration CC between the Swiss Institute of Bioinformatics and the EMBL outstation - CC the European Bioinformatics Institute. There are no restrictions on its CC use by non-profit institutions as long as its content is in no way CC modified and this statement is not removed. Usage by and for commercial CC entities requires a license agreement (See http://www.isb-sib.ch/announce/ CC or send an email to license@isb-sib.ch). CC -------------------------------------------------------------------------- CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE000299; AAC75164.1; -. DR EMBL; D90848; CAB21893.1; -. DR EMBL; D84200; BAA76742.1; -. DR ECOGENE; EG20225; THID. KW Thiamine biosynthesis; Transferase; Kinase. SQ SEQUENCE 266 AA; 28633 MW; E555CEFE445B8F0A CRC64; MKRINALTIA GTDPSGGAGI QADLKTFSAL GAYGCSVITA LVAQNTRGVQ SVYRIEPDFV AAQLDSVFSD VRIDTTKIGM LAETDIVEAV AERLQRYQIQ NVVLDTVMLA KSGDPLLSPS AVATLRSRLL PQVSLITPNL PEAAALLDAP HARTEQEMLE QGRSLLAMGC GAVLMKGGHL DDEQSPDWLF TREGEQRFTA PRIMTKNTHG TGCTLSAALA ALRPRHTNWA DTVQEAKSWL SSALAQADTL EVGHGIGPVH HFHAWW //