ID TIAR_MOUSE Reviewed; 392 AA. AC P70318; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1997, sequence version 1. DT 10-FEB-2021, entry version 153. DE RecName: Full=Nucleolysin TIAR; DE AltName: Full=TIA-1-related protein; GN Name=Tial1; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=8871565; DOI=10.1093/nar/24.19.3829; RA Beck A.R.P., Medley O.G., O'Brien S., Anderson P., Streuli M.; RT "Structure, tissue distribution and genomic organization of the murine RRM- RT type RNA binding proteins TIA-1 and TIAR."; RL Nucleic Acids Res. 24:3829-3836(1996). RN [2] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Brain, Lung, Spleen, and Testis; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). RN [3] RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX PubMed=19861488; DOI=10.1530/rep-09-0373; RA Yamaji M., Tanaka T., Shigeta M., Chuma S., Saga Y., Saitou M.; RT "Functional reconstruction of NANOS3 expression in the germ cell lineage by RT a novel transgenic reporter reveals distinct subcellular localizations of RT NANOS3."; RL Reproduction 139:381-393(2010). CC -!- FUNCTION: RNA-binding protein. Possesses nucleolytic activity against CC cytotoxic lymphocyte target cells. May be involved in apoptosis (By CC similarity). {ECO:0000250}. CC -!- INTERACTION: CC P70318; Q8K2L4: Ddx21; NbExp=2; IntAct=EBI-299820, EBI-7977861; CC P70318; Q3THB3: Hnrnpm; NbExp=2; IntAct=EBI-299820, EBI-4282050; CC P70318; Q6PDM2: Srsf1; NbExp=3; IntAct=EBI-299820, EBI-2550360; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19861488}. Nucleus CC {ECO:0000269|PubMed:19861488}. Cytoplasmic granule CC {ECO:0000269|PubMed:19861488}. Note=The cytoplasmic granules are stress CC granules which are a dense aggregation in the cytosol composed of CC proteins and RNAs that appear when the cell is under stress. CC Colocalizes with NANOS3 in the stress granules. CC -!- TISSUE SPECIFICITY: Expressed both in primordial germ cells (PGCs) and CC in neighboring somatic cells. {ECO:0000269|PubMed:19861488}. CC -!- PTM: Phosphorylated by MAPK14 following DNA damage, releasing TIAR from CC GADD45A mRNA. {ECO:0000250}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U55861; AAC52870.1; -; mRNA. DR CCDS; CCDS21897.1; -. DR PIR; S72436; S72436. DR RefSeq; NP_033409.1; NM_009383.2. DR SMR; P70318; -. DR BioGRID; 204192; 14. DR IntAct; P70318; 28. DR MINT; P70318; -. DR STRING; 10090.ENSMUSP00000101833; -. DR iPTMnet; P70318; -. DR PhosphoSitePlus; P70318; -. DR EPD; P70318; -. DR PaxDb; P70318; -. DR PeptideAtlas; P70318; -. DR PRIDE; P70318; -. DR Antibodypedia; 18838; 137 antibodies. DR Ensembl; ENSMUST00000106226; ENSMUSP00000101833; ENSMUSG00000030846. DR GeneID; 21843; -. DR KEGG; mmu:21843; -. DR UCSC; uc009jyy.1; mouse. DR CTD; 7073; -. DR MGI; MGI:107913; Tial1. DR eggNOG; KOG0148; Eukaryota. DR GeneTree; ENSGT00940000160482; -. DR InParanoid; P70318; -. DR OrthoDB; 1066369at2759; -. DR PhylomeDB; P70318; -. DR TreeFam; TF312915; -. DR Reactome; R-MMU-6803529; FGFR2 alternative splicing. DR BioGRID-ORCS; 21843; 1 hit in 18 CRISPR screens. DR ChiTaRS; Tial1; mouse. DR PRO; PR:P70318; -. DR Proteomes; UP000000589; Chromosome 7. DR RNAct; P70318; protein. DR Bgee; ENSMUSG00000030846; Expressed in ureter smooth muscle and 312 other tissues. DR Genevisible; P70318; MM. DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB. DR GO; GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB. DR GO; GO:0005634; C:nucleus; IDA:UniProtKB. DR GO; GO:1990904; C:ribonucleoprotein complex; ISO:MGI. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0035925; F:mRNA 3'-UTR AU-rich region binding; IDA:MGI. DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW. DR GO; GO:0007281; P:germ cell development; IMP:MGI. DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:MGI. DR GO; GO:0017145; P:stem cell division; IMP:MGI. DR CDD; cd12616; RRM1_TIAR; 1. DR CDD; cd12617; RRM2_TIAR; 1. DR CDD; cd12620; RRM3_TIAR; 1. DR Gene3D; 3.30.70.330; -; 3. DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf. DR InterPro; IPR035979; RBD_domain_sf. DR InterPro; IPR000504; RRM_dom. DR InterPro; IPR003954; RRM_dom_euk. DR InterPro; IPR034492; TIAR_RRM1. DR InterPro; IPR034494; TIAR_RRM2. DR InterPro; IPR034496; TIAR_RRM3. DR Pfam; PF00076; RRM_1; 4. DR SMART; SM00360; RRM; 3. DR SMART; SM00361; RRM_1; 3. DR SUPFAM; SSF54928; SSF54928; 3. DR PROSITE; PS50102; RRM; 3. PE 1: Evidence at protein level; KW Acetylation; Apoptosis; Cytoplasm; Nucleus; Phosphoprotein; KW Reference proteome; Repeat; RNA-binding. FT CHAIN 1..392 FT /note="Nucleolysin TIAR" FT /id="PRO_0000081979" FT DOMAIN 9..102 FT /note="RRM 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176" FT DOMAIN 114..192 FT /note="RRM 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176" FT DOMAIN 222..294 FT /note="RRM 3" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176" FT MOD_RES 139 FT /note="N6-acetyllysine" FT /evidence="ECO:0000250|UniProtKB:Q01085" FT MOD_RES 218 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:Q01085" SQ SEQUENCE 392 AA; 43389 MW; 66E6AF14B9EBE77A CRC64; MMEDDGQPRT LYVGNLSRDV TEVLILQLFS QIGPCKSCKM ITEQPDSRRV NSSVGFSVLQ HTSNDPYCFV EFYEHRDAAA ALAAMNGRKI LGKEVKVNWA TTPSSQKKDT SNHFHVFVGD LSPEITTEDI KSAFAPFGKI SDARVVKDMA TGKSKGYGFV SFYNKLDAEN AIVHMGGQWL GGRQIRTNWA TRKPPAPKST QETNTKQLRF EDVVNQSSPK NCTVYCGGIA SGLTDQLMRQ TFSPFGQIME IRVFPEKGYS FVRFSTHESA AHAIVSVNGT TIEGHVVKCY WGKESPDMTK NFQQVDYSQW GQWSQVYGNP QQYGQYMANG WQVPPYGVYG QPWNQQGFGV DQSPSAAWMG GFGAQPPQGQ APPPVIPPPN QAGYGMASFP TQ //