ID RECG_MYCBO Reviewed; 737 AA. AC P64323; A0A1R3Y2R4; P95122; X2BMQ8; DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot. DT 11-OCT-2004, sequence version 1. DT 24-JAN-2024, entry version 112. DE RecName: Full=ATP-dependent DNA helicase RecG; DE EC=3.6.4.12; GN Name=recG; OrderedLocusNames=BQ2027_MB2998C; OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97). OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae; OC Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=233413; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC BAA-935 / AF2122/97; RX PubMed=12788972; DOI=10.1073/pnas.1130426100; RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J., RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.; RT "The complete genome sequence of Mycobacterium bovis."; RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION. RC STRAIN=ATCC BAA-935 / AF2122/97; RX PubMed=28385856; DOI=10.1128/genomea.00157-17; RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R., RA Robbe-Austerman S., Gordon S.V.; RT "Updated reference genome sequence and annotation of Mycobacterium bovis RT AF2122/97."; RL Genome Announc. 5:E00157-E00157(2017). CC -!- FUNCTION: Critical role in recombination and DNA repair. Helps process CC Holliday junction intermediates to mature products by catalyzing branch CC migration. Has a DNA unwinding activity characteristic of a DNA CC helicase with a 3'- to 5'- polarity. Unwinds branched duplex DNA (Y- CC DNA) (By similarity). {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC -!- SIMILARITY: Belongs to the helicase family. RecG subfamily. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LT708304; SIU01621.1; -; Genomic_DNA. DR RefSeq; NP_856643.1; NC_002945.3. DR RefSeq; WP_003415026.1; NC_002945.4. DR AlphaFoldDB; P64323; -. DR SMR; P64323; -. DR PATRIC; fig|233413.5.peg.3295; -. DR OMA; DNGFQAC; -. DR Proteomes; UP000001419; Chromosome. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro. DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW. DR CDD; cd17992; DEXHc_RecG; 1. DR CDD; cd04488; RecG_wedge_OBF; 1. DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2. DR InterPro; IPR004609; ATP-dep_DNA_helicase_RecG. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR047112; RecG/Mfd. DR InterPro; IPR033454; RecG_wedge. DR NCBIfam; TIGR00643; recG; 1. DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1. DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; Helicase_C; 1. DR Pfam; PF17191; RecG_wedge; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. PE 3: Inferred from homology; KW ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding; KW Helicase; Hydrolase; Nucleotide-binding; Reference proteome. FT CHAIN 1..737 FT /note="ATP-dependent DNA helicase RecG" FT /id="PRO_0000102147" FT DOMAIN 302..477 FT /note="Helicase ATP-binding" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541" FT DOMAIN 514..673 FT /note="Helicase C-terminal" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542" FT MOTIF 427..430 FT /note="DEQH box" FT BINDING 315..322 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541" SQ SEQUENCE 737 AA; 80361 MW; 97BAC1C8C4A0732F CRC64; MASLSDRLDR VLGATAADAL DEQFGMRTVD DLLRHYPRSY VEGAARVGIG DARPEAGEHI TIVDVITDTY SFPMKKKPNR KCLRITVGGG RNKVTATFFN ADYIMRDLTK HTKVMLSGEV GYYKGAMQLT HPAFLILDSP DGKNHGTRSL KSIADASKAI SGELVVEEFE RRFFPIYPAS TKVQSWDIFK CVRQVLDVLD RVDDPLPAEL RAKHGLIPED EALRAIHLAE SQSLRERARE RLTFDEAVGL QWALVARRHG ELSESGPSAA WKSNGLAAEL LRRLPFELTA GQREVLDVLS DGLAANRPLN RLLQGEVGSG KTIVAVLAML QMVDAGYQCA LLAPTEVLAA QHLRSIRDVL GPLAMGGQLG GAENATRVAL LTGSMTAGQK KQVRAEIASG QVGIVIGTHA LLQEAVDFHN LGMVVVDEQH RFGVEQRDQL RAKAPAGITP HLLVMTATPI PRTVALTVYG DLETSTLREL PLGRQPIATN VIFVKDKPAW LDRAWRRIIE EAAAGRQAYV VAPRIDESDD TDVQGGVRPS ATAEGLFSRL RSAELAELRL ALMHGRLSAD DKDAAMAAFR AGEVDVLVCT TVIEVGVDVP NATVMLVMDA DRFGISQLHQ LRGRIGRGEH PSVCLLASWV PPDTPAGQRL RAVAGTMDGF ALADLDLKER KEGDVLGRNQ SGKAITLRLL SLAEHEEYIV AARDFCIEAY KNPTDPALAL MAARFTSTDR IEYLDKS //