ID RUXF_HUMAN Reviewed; 86 AA. AC P62306; A2VCR2; B2R498; Q15356; Q6IBQ1; Q6P4I0; DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot. DT 05-JUL-2004, sequence version 1. DT 13-JUL-2010, entry version 65. DE RecName: Full=Small nuclear ribonucleoprotein F; DE Short=snRNP-F; DE AltName: Full=Sm protein F; DE Short=Sm-F; DE Short=SmF; GN Name=SNRPF; Synonyms=PBSCF; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Cervix carcinoma; RX MEDLINE=95262647; PubMed=7744013; RA Hermann H., Fabrizio P., Raker V.A., Foulaki K., Hornig H., Brahms H., RA Luehrmann R.; RT "snRNP Sm proteins share two evolutionarily conserved sequence motifs RT which are involved in Sm protein-protein interactions."; RL EMBO J. 14:2076-2088(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.; RT "Cloning of human full open reading frames in Gateway(TM) system entry RT vector (pDONR201)."; RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Amygdala; RX PubMed=14702039; DOI=10.1038/ng1285; RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., RA Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., RA Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., RA Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., RA Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., RA Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., RA Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., RA Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., RA Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., RA Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., RA Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., RA Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., RA Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., RA Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., RA Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., RA Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., RA Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., RA Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., RA Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., RA Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.; RT "Complete sequencing and characterization of 21,243 full-length human RT cDNAs."; RL Nat. Genet. 36:40-45(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., RA Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., RA Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., RA Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R., RA Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., RA Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., RA Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., RA Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Colon; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [6] RP PROTEIN SEQUENCE OF 2-22 AND 66-86, CLEAVAGE OF INITIATOR METHIONINE, RP ACETYLATION AT SER-2, AND MASS SPECTROMETRY. RC TISSUE=Ovarian carcinoma; RA Bienvenut W.V., Lilla S., von Kriegsheim A., Lempens A., Kolch W.; RL Submitted (DEC-2008) to UniProtKB. RN [7] RP IDENTIFICATION IN THE U7 SNRNP COMPLEX. RX MEDLINE=21458437; PubMed=11574479; DOI=10.1093/emboj/20.19.5470; RA Pillai R.S., Will C.L., Luehrmann R., Schuemperli D., Mueller B.; RT "Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, RT a new 14 kDa Sm D1-like protein."; RL EMBO J. 20:5470-5479(2001). RN [8] RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE RP SPLICEOSOMAL C COMPLEX. RX PubMed=11991638; DOI=10.1017/S1355838202021088; RA Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.; RT "Purification and characterization of native spliceosomes suitable for RT three-dimensional structural analysis."; RL RNA 8:426-439(2002). RN [9] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RA Colinge J., Superti-Furga G., Bennett K.L.; RL Submitted (OCT-2008) to UniProtKB. CC -!- FUNCTION: Appears to function in the U7 snRNP complex that is CC involved in histone 3'-end processing. Associated with snRNP U1, CC U2, U4/U6 and U5. CC -!- SUBUNIT: Component of the heptameric ring U7 snRNP complex, or U7 CC Sm protein core complex, at least composed of LSM10, LSM11, SNRPB, CC SNRPD3, SNRPE, SNRPF, SNRPG and U7 snRNA. Formation of the U7 CC snRNP is an ATP-dependent process mediated by a specialized SMN CC complex containing at least the Sm protein core complex and CC additionally, the U7-specific LSM10 and LSM11 proteins. Identified CC in the spliceosome C complex, at least composed of AQR, ASCC3L1, CC C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, CC DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, CC HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, CC KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, CC PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, CC RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, CC SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, CC SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, CC TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. CC -!- INTERACTION: CC O95400:CD2BP2; NbExp=1; IntAct=EBI-356900, EBI-768015; CC Q9Y4Y9:LSM5; NbExp=1; IntAct=EBI-356900, EBI-373007; CC O15527:OGG1; NbExp=1; IntAct=EBI-356900, EBI-721675; CC P31930:UQCRC1; NbExp=1; IntAct=EBI-356900, EBI-1052596; CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- SIMILARITY: Belongs to the snRNP Sm proteins family. SmF/LSm6 CC subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X85372; CAA59688.1; -; mRNA. DR EMBL; CR456751; CAG33032.1; -; mRNA. DR EMBL; AK311752; BAG34695.1; -; mRNA. DR EMBL; CH471054; EAW97548.1; -; Genomic_DNA. DR EMBL; BC002505; AAH02505.3; -; mRNA. DR EMBL; BC063397; AAH63397.2; -; mRNA. DR EMBL; BC128452; AAI28453.1; -; mRNA. DR EMBL; BC128453; AAI28454.1; -; mRNA. DR IPI; IPI00220528; -. DR PIR; S55053; S55053. DR RefSeq; NP_003086.1; -. DR UniGene; Hs.105465; -. DR PDB; 3CW1; X-ray; 5.49 A; 1/2/F/Z=1-86. DR PDBsum; 3CW1; -. DR IntAct; P62306; 14. DR MINT; MINT-1137229; -. DR STRING; P62306; -. DR PeptideAtlas; P62306; -. DR PRIDE; P62306; -. DR Ensembl; ENST00000266735; ENSP00000266735; ENSG00000139343; Homo sapiens. DR GeneID; 6636; -. DR KEGG; hsa:6636; -. DR UCSC; uc001tej.1; human. DR CTD; 6636; -. DR GeneCards; GC12P094776; -. DR H-InvDB; HIX0036777; -. DR HGNC; HGNC:11162; SNRPF. DR MIM; 603541; gene. DR PharmGKB; PA36003; -. DR eggNOG; prNOG21099; -. DR HOVERGEN; HBG052369; -. DR InParanoid; P62306; -. DR OMA; AVDSYMN; -. DR PhylomeDB; P62306; -. DR Reactome; REACT_125; Processing of Capped Intron-Containing Pre-mRNA. DR Reactome; REACT_21257; Metabolism of RNA. DR Reactome; REACT_71; Gene Expression. DR NextBio; 25853; -. DR PMAP-CutDB; P62306; -. DR ArrayExpress; P62306; -. DR Bgee; P62306; -. DR CleanEx; HS_SNRPF; -. DR Genevestigator; P62306; -. DR GermOnline; ENSG00000139343; Homo sapiens. DR GO; GO:0005829; C:cytosol; EXP:Reactome. DR GO; GO:0005654; C:nucleoplasm; EXP:Reactome. DR GO; GO:0005681; C:spliceosomal complex; IDA:UniProtKB. DR GO; GO:0005683; C:U7 snRNP; IDA:UniProtKB. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0003723; F:RNA binding; IMP:UniProtKB. DR GO; GO:0000387; P:spliceosomal snRNP assembly; EXP:Reactome. DR InterPro; IPR001163; LSM_domain. DR InterPro; IPR006649; LSM_domain_euk/arc. DR InterPro; IPR010920; LSM_related_domain. DR InterPro; IPR016487; snRNP_SmF. DR Pfam; PF01423; LSM; 1. DR PIRSF; PIRSF006609; snRNP_SmF; 1. DR SMART; SM00651; Sm; 1. DR SUPFAM; SSF50182; Sm_like_riboprot; 1. PE 1: Evidence at protein level; KW 3D-structure; Acetylation; Complete proteome; KW Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; KW Ribonucleoprotein; RNA-binding; Spliceosome. FT INIT_MET 1 1 Removed. FT CHAIN 2 86 Small nuclear ribonucleoprotein F. FT /FTId=PRO_0000125536. FT MOD_RES 2 2 N-acetylserine. FT CONFLICT 86 86 E -> D (in Ref. 2; CAG33032). SQ SEQUENCE 86 AA; 9725 MW; 416B88D575DAE58A CRC64; MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE //