ID   T2R30_HUMAN             Reviewed;         319 AA.
AC   P59541; Q645X7;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 3.
DT   05-FEB-2025, entry version 135.
DE   RecName: Full=Taste receptor type 2 member 30;
DE            Short=T2R30;
DE   AltName: Full=Taste receptor type 2 member 47;
DE            Short=T2R47;
GN   Name=TAS2R30; Synonyms=TAS2R47;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT LEU-252.
RX   PubMed=15496549; DOI=10.1093/molbev/msi027;
RA   Fischer A., Gilad Y., Man O., Paeaebo S.;
RT   "Evolution of bitter taste receptors in humans and apes.";
RL   Mol. Biol. Evol. 22:432-436(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16541075; DOI=10.1038/nature04569;
RA   Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA   Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA   Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA   Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA   Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA   Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA   Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA   Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA   Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA   Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA   Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA   Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA   Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA   Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA   Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA   Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA   Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA   David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA   D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA   Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA   Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA   Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA   LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA   Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA   Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA   Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA   Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA   Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA   Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA   Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA   Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA   Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA   Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA   Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA   Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA   Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA   Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA   Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA   Gibbs R.A.;
RT   "The finished DNA sequence of human chromosome 12.";
RL   Nature 440:346-351(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-309.
RX   PubMed=12379855; DOI=10.1038/ng1014;
RA   Bufe B., Hofmann T., Krautwurst D., Raguse J.-D., Meyerhof W.;
RT   "The human TAS2R16 receptor mediates bitter taste in response to beta-
RT   glucopyranosides.";
RL   Nat. Genet. 32:397-401(2002).
RN   [4]
RP   REVIEW.
RX   PubMed=12139982; DOI=10.1016/s0959-4388(02)00345-8;
RA   Montmayeur J.-P., Matsunami H.;
RT   "Receptors for bitter and sweet taste.";
RL   Curr. Opin. Neurobiol. 12:366-371(2002).
RN   [5]
RP   REVIEW.
RX   PubMed=11696554; DOI=10.1074/jbc.r100054200;
RA   Margolskee R.F.;
RT   "Molecular mechanisms of bitter and sweet taste transduction.";
RL   J. Biol. Chem. 277:1-4(2002).
RN   [6]
RP   REVIEW.
RX   PubMed=12581520; DOI=10.1016/s0092-8674(03)00071-0;
RA   Zhang Y., Hoon M.A., Chandrashekar J., Mueller K.L., Cook B., Wu D.,
RA   Zuker C.S., Ryba N.J.;
RT   "Coding of sweet, bitter, and umami tastes: different receptor cells
RT   sharing similar signaling pathways.";
RL   Cell 112:293-301(2003).
CC   -!- FUNCTION: Receptor that may play a role in the perception of bitterness
CC       and is gustducin-linked. May play a role in sensing the chemical
CC       composition of the gastrointestinal content. The activity of this
CC       receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation
CC       and lead to the gating of TRPM5 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in subsets of taste receptor cells of the
CC       tongue and exclusively in gustducin-positive cells.
CC   -!- MISCELLANEOUS: Most taste cells may be activated by a limited number of
CC       bitter compounds; individual taste cells can discriminate among bitter
CC       stimuli.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor T2R family.
CC       {ECO:0000305}.
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DR   EMBL; AY724940; AAU21142.1; -; Genomic_DNA.
DR   EMBL; AF494233; AAM19324.1; -; Genomic_DNA.
DR   CCDS; CCDS53750.1; -.
DR   RefSeq; NP_001091112.1; NM_001097643.1.
DR   AlphaFoldDB; P59541; -.
DR   SMR; P59541; -.
DR   STRING; 9606.ENSP00000444736; -.
DR   ChEMBL; CHEMBL4523254; -.
DR   DrugCentral; P59541; -.
DR   GuidetoPHARMACOLOGY; 673; -.
DR   GlyCosmos; P59541; 2 sites, No reported glycans.
DR   GlyGen; P59541; 2 sites, 1 N-linked glycan (1 site).
DR   iPTMnet; P59541; -.
DR   PhosphoSitePlus; P59541; -.
DR   BioMuta; TAS2R30; -.
DR   DMDM; 55977810; -.
DR   MassIVE; P59541; -.
DR   PaxDb; 9606-ENSP00000444736; -.
DR   PeptideAtlas; P59541; -.
DR   Antibodypedia; 57635; 20 antibodies from 10 providers.
DR   DNASU; 259293; -.
DR   Ensembl; ENST00000539585.1; ENSP00000444736.1; ENSG00000256188.3.
DR   Ensembl; ENST00000576640.1; ENSP00000458170.1; ENSG00000262111.3.
DR   GeneID; 259293; -.
DR   KEGG; hsa:259293; -.
DR   MANE-Select; ENST00000539585.1; ENSP00000444736.1; NM_001097643.2; NP_001091112.1.
DR   UCSC; uc009zhs.2; human.
DR   AGR; HGNC:19112; -.
DR   CTD; 259293; -.
DR   GeneCards; TAS2R30; -.
DR   HGNC; HGNC:19112; TAS2R30.
DR   HPA; ENSG00000256188; Not detected.
DR   MIM; 613963; gene.
DR   neXtProt; NX_P59541; -.
DR   OpenTargets; ENSG00000256188; -.
DR   VEuPathDB; HostDB:ENSG00000256188; -.
DR   eggNOG; ENOG502TE6U; Eukaryota.
DR   GeneTree; ENSGT01100000263477; -.
DR   HOGENOM; CLU_072337_2_0_1; -.
DR   InParanoid; P59541; -.
DR   OMA; HFSSWLA; -.
DR   OrthoDB; 9484230at2759; -.
DR   PhylomeDB; P59541; -.
DR   PathwayCommons; P59541; -.
DR   Reactome; R-HSA-418594; G alpha (i) signalling events.
DR   Reactome; R-HSA-420499; Class C/3 (Metabotropic glutamate/pheromone receptors).
DR   Reactome; R-HSA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR   BioGRID-ORCS; 259293; 12 hits in 1066 CRISPR screens.
DR   GenomeRNAi; 259293; -.
DR   Pharos; P59541; Tchem.
DR   PRO; PR:P59541; -.
DR   Proteomes; UP000005640; Chromosome 12.
DR   RNAct; P59541; protein.
DR   Bgee; ENSG00000256188; Expressed in corpus callosum and 98 other cell types or tissues.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0033038; F:bitter taste receptor activity; IDA:UniProtKB.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; IEA:UniProtKB-KW.
DR   GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IDA:UniProtKB.
DR   CDD; cd15027; 7tm_TAS2R43-like; 1.
DR   FunFam; 1.20.1070.10:FF:000042; Taste receptor type 2 member 7; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR007960; TAS2R.
DR   PANTHER; PTHR11394; TASTE RECEPTOR TYPE 2; 1.
DR   PANTHER; PTHR11394:SF48; TASTE RECEPTOR TYPE 2 MEMBER 30; 1.
DR   Pfam; PF05296; TAS2R; 1.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
PE   2: Evidence at transcript level;
KW   G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW   Reference proteome; Sensory transduction; Taste; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..319
FT                   /note="Taste receptor type 2 member 30"
FT                   /id="PRO_0000082323"
FT   TOPO_DOM        1
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        2..22
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        23..46
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..67
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        68..86
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        108..126
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        148..178
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        200..229
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        251..259
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        281..319
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        161
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        176
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         252
FT                   /note="F -> L (in dbSNP:rs2599404)"
FT                   /evidence="ECO:0000269|PubMed:15496549"
FT                   /id="VAR_070809"
FT   CONFLICT        314..319
FT                   /note="GALCVF -> AALCKG (in Ref. 1; AAU21142)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   319 AA;  36874 MW;  EEC11DE01E0C98F3 CRC64;
     MITFLPIIFS ILIVVIFVIG NFANGFIALV NSIEWVKRQK ISFVDQILTA LAVSRVGLLW
     VLLLHWYATQ LNPAFYSVEV RITAYNVWAV TNHFSSWLAT SLSMFYLLRI ANFSNLIFLR
     IKRRVKSVVL VILLGPLLFL VCHLFVINMD ETVWTKEYEG NVTWKIKLRS AMYHSNMTLT
     MLANFVPLTL TLISFLLLIC SLCKHLKKMQ LHGKGSQDPS TKVHIKALQT VTSFLLLCAI
     YFLSMIISVC NFGRLEKQPV FMFCQAIIFS YPSTHPFILI LGNKKLKQIF LSVLRHVRYW
     VKDRSLRLHR FTRGALCVF
//