ID DPO4_THETN STANDARD; PRT; 384 AA. AC P58965; Q8RD00; DT 28-FEB-2003 (Rel. 41, Created) DT 28-FEB-2003 (Rel. 41, Last sequence update) DT 01-MAY-2005 (Rel. 47, Last annotation update) DE DNA polymerase IV (EC 2.7.7.7) (Pol IV). GN Name=dinB; OrderedLocusNames=TTE0254; OS Thermoanaerobacter tengcongensis. OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacteriales; OC Thermoanaerobacteriaceae; Thermoanaerobacter. OX NCBI_TaxID=119072; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MB4 / JCM 11007; RX MEDLINE=21992816; PubMed=11997336; DOI=10.1101/gr.219302; RA Bao Q., Tian Y., Li W., Xu Z., Xuan Z., Hu S., Dong W., Yang J., RA Chen Y., Xue Y., Xu Y., Lai X., Huang L., Dong X., Ma Y., Ling L., RA Tan H., Chen R., Wang J., Yu J., Yang H.; RT "A complete sequence of the T. tengcongensis genome."; RL Genome Res. 12:689-700(2002). CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved CC in untargeted mutagenesis. Copies undamaged DNA at stalled CC replication forks, which arise in vivo from mismatched or CC misaligned primer ends. These misaligned primers can be extended CC by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity. CC May be involved in translesional synthesis, in conjunction with CC the beta clamp from polIII (By similarity). CC -!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = CC diphosphate + DNA(n+1). CC -!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity). CC -!- SUBUNIT: Monomer (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasmic (Probable). CC -!- SIMILARITY: Belongs to the DNA polymerase type-Y family. CC -!- SIMILARITY: Contains 1 umuC domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE012997; AAM23550.1; -. DR HSSP; P96022; 1K1Q. DR HAMAP; MF_01113; -; 1. DR InterPro; IPR001126; UMUC_like. DR Pfam; PF00817; IMS; 1. DR PROSITE; PS50173; UMUC; 1. KW Complete proteome; DNA damage; DNA repair; DNA replication; KW DNA-binding; DNA-directed DNA polymerase; Magnesium; Metal-binding; KW Mutator protein; Nucleotidyltransferase; Transferase. FT DOMAIN 5 182 UmuC. FT SITE 14 14 Substrate discrimination (By similarity). FT ACT_SITE 104 104 By similarity. FT METAL 9 9 Magnesium (By similarity). FT METAL 103 103 Magnesium (By similarity). SQ SEQUENCE 384 AA; 43916 MW; 01C214516AD9AABB CRC64; MKRKIIHVDM DAFFASIEQQ DNPEYRGKPV IVGGLSGRGV VSTCSYEARK YGIHSAMPMY MAKKLCPQGI FLPVRRKRYE EVSEQIFRIL YDITPFVEPV SIDEAYLDVT HVDKNPEDIA LEIKKRVKDA TGLTVSVGIS YNKFLAKLAS DWNKPDGLMV ITEDMVPEIL KPLPVTKVHG IGEKSAEKLR SIGIETVEDL LKLPQENLIE LFGKTGVEIY NRIRGIDERP VETMREIKSI GKEKTLEKDT KNKELLIQHL KEFSEIVSEE LIKERLYCRT VTVKIKTADF AVHTKSKTVD KYIRFSEDIY EVAKGILEEW KLEQYVRLIG LSVSNLSPVK YEQLSFLDKR LVKVIKAGNL AEEINKRIGK KIIKKGSELL KDNK //