ID CLD18_MOUSE Reviewed; 264 AA. AC P56857; Q91ZY9; Q91ZZ0; Q91ZZ1; DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot. DT 30-MAY-2000, sequence version 1. DT 12-AUG-2020, entry version 136. DE RecName: Full=Claudin-18; GN Name=Cldn18; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND RP ALTERNATIVE SPLICING. RX PubMed=11585919; DOI=10.1128/mcb.21.21.7380-7390.2001; RA Niimi T., Nagashima K., Ward J.M., Minoo P., Zimonjic D.B., Popescu N.C., RA Kimura S.; RT "Claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 RT homeodomain transcription factor, encodes lung- and stomach-specific RT isoforms through alternative splicing."; RL Mol. Cell. Biol. 21:7380-7390(2001). RN [2] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Lung; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). CC -!- FUNCTION: Plays a major role in tight junction-specific obliteration of CC the intercellular space, through calcium-independent cell-adhesion CC activity. {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Cell junction, tight junction CC {ECO:0000269|PubMed:11585919}. Cell membrane CC {ECO:0000269|PubMed:11585919}; Multi-pass membrane protein CC {ECO:0000255}. Note=Localizes to tight junctions in epithelial cells. CC {ECO:0000269|PubMed:11585919}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=4; CC Name=A1.1; CC IsoId=P56857-1; Sequence=Displayed; CC Name=A1.2; CC IsoId=P56857-2; Sequence=VSP_001104, VSP_001105; CC Name=A2.1; CC IsoId=P56857-3; Sequence=VSP_001103; CC Name=A2.2; CC IsoId=P56857-4; Sequence=VSP_001103, VSP_001104, VSP_001105; CC -!- TISSUE SPECIFICITY: Isoform A1.1: Expressed in lung (PubMed:11585919). CC Isoform A1.2: Expressed in lung (PubMed:11585919). Isoform A2.1: CC Expressed in stomach (PubMed:11585919). Isoform A2.2: Expressed in CC stomach (PubMed:11585919). CC -!- SIMILARITY: Belongs to the claudin family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF221068; AAF26447.1; -; mRNA. DR EMBL; AF349450; AAL15635.1; -; mRNA. DR EMBL; AF349451; AAL15636.1; -; mRNA. DR EMBL; AF349453; AAL15638.1; -; mRNA. DR CCDS; CCDS23437.1; -. [P56857-1] DR CCDS; CCDS57692.1; -. [P56857-2] DR CCDS; CCDS57693.1; -. [P56857-4] DR CCDS; CCDS57694.1; -. [P56857-3] DR RefSeq; NP_001181850.1; NM_001194921.1. [P56857-3] DR RefSeq; NP_001181851.1; NM_001194922.1. [P56857-2] DR RefSeq; NP_001181852.1; NM_001194923.1. [P56857-4] DR RefSeq; NP_062789.1; NM_019815.3. [P56857-1] DR DIP; DIP-48956N; -. DR IntAct; P56857; 3. DR MINT; P56857; -. DR STRING; 10090.ENSMUSP00000035048; -. DR TCDB; 1.H.1.1.8; the claudin tight junction (claudin1) family. DR iPTMnet; P56857; -. DR PhosphoSitePlus; P56857; -. DR MaxQB; P56857; -. DR PaxDb; P56857; -. DR PRIDE; P56857; -. DR Antibodypedia; 4575; 193 antibodies. DR DNASU; 56492; -. DR Ensembl; ENSMUST00000035048; ENSMUSP00000035048; ENSMUSG00000032473. [P56857-1] DR Ensembl; ENSMUST00000112882; ENSMUSP00000108503; ENSMUSG00000032473. [P56857-3] DR Ensembl; ENSMUST00000131922; ENSMUSP00000117382; ENSMUSG00000032473. [P56857-4] DR Ensembl; ENSMUST00000136429; ENSMUSP00000115782; ENSMUSG00000032473. [P56857-2] DR GeneID; 56492; -. DR KEGG; mmu:56492; -. DR UCSC; uc009rep.2; mouse. [P56857-1] DR UCSC; uc009req.2; mouse. [P56857-2] DR UCSC; uc009rer.2; mouse. [P56857-4] DR UCSC; uc009res.2; mouse. [P56857-3] DR CTD; 51208; -. DR MGI; MGI:1929209; Cldn18. DR eggNOG; ENOG502QTRB; Eukaryota. DR GeneTree; ENSGT00940000158655; -. DR HOGENOM; CLU_076370_2_1_1; -. DR InParanoid; P56857; -. DR KO; K06087; -. DR OMA; QYQGLWR; -. DR PhylomeDB; P56857; -. DR TreeFam; TF331936; -. DR BioGRID-ORCS; 56492; 0 hits in 18 CRISPR screens. DR PRO; PR:P56857; -. DR Proteomes; UP000000589; Chromosome 9. DR RNAct; P56857; protein. DR Bgee; ENSMUSG00000032473; Expressed in lung and 55 other tissues. DR Genevisible; P56857; MM. DR GO; GO:0005923; C:bicellular tight junction; ISS:UniProtKB. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central. DR GO; GO:0042802; F:identical protein binding; ISS:UniProtKB. DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro. DR GO; GO:0070830; P:bicellular tight junction assembly; IBA:GO_Central. DR GO; GO:0016338; P:calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules; ISS:UniProtKB. DR GO; GO:0007155; P:cell adhesion; IBA:GO_Central. DR GO; GO:0048565; P:digestive tract development; IMP:MGI. DR GO; GO:0045779; P:negative regulation of bone resorption; IDA:MGI. DR GO; GO:2001205; P:negative regulation of osteoclast development; IDA:MGI. DR GO; GO:1900181; P:negative regulation of protein localization to nucleus; IDA:MGI. DR GO; GO:0045471; P:response to ethanol; IEA:Ensembl. DR GO; GO:0071847; P:TNFSF11-mediated signaling pathway; IMP:MGI. DR InterPro; IPR006187; Claudin. DR InterPro; IPR003928; Claudin18. DR InterPro; IPR017974; Claudin_CS. DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin. DR PANTHER; PTHR12002; PTHR12002; 1. DR PANTHER; PTHR12002:SF9; PTHR12002:SF9; 1. DR Pfam; PF00822; PMP22_Claudin; 1. DR PRINTS; PR01448; CLAUDIN18. DR PROSITE; PS01346; CLAUDIN; 1. PE 1: Evidence at protein level; KW Alternative splicing; Cell junction; Cell membrane; Membrane; KW Phosphoprotein; Reference proteome; Tight junction; Transmembrane; KW Transmembrane helix. FT CHAIN 1..264 FT /note="Claudin-18" FT /id="PRO_0000144780" FT TOPO_DOM 1..6 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 7..27 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 28..80 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 81..101 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 102..122 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 123..143 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 144..176 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 177..197 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 198..264 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT MOD_RES 217 FT /note="Phosphoserine" FT /evidence="ECO:0000244|PubMed:21183079" FT VAR_SEQ 1..69 FT /note="MATTTCQVVGLLLSLLGLAGCIAATGMDMWSTQDLYDNPVTAVFQYEGLWRS FT CVQQSSGFTECRPYFTI -> MSVTACQGLGFVVSLIGFAGIIAATCMDQWSTQDLYNN FT PVTAVFNYQGLWRSCVRESSGFTECRGYFTL (in isoform A2.1 and FT isoform A2.2)" FT /evidence="ECO:0000305" FT /id="VSP_001103" FT VAR_SEQ 208 FT /note="N -> K (in isoform A1.2 and isoform A2.2)" FT /evidence="ECO:0000305" FT /id="VSP_001104" FT VAR_SEQ 209..264 FT /note="Missing (in isoform A1.2 and isoform A2.2)" FT /evidence="ECO:0000305" FT /id="VSP_001105" SQ SEQUENCE 264 AA; 28122 MW; 3CA0D441C4705653 CRC64; MATTTCQVVG LLLSLLGLAG CIAATGMDMW STQDLYDNPV TAVFQYEGLW RSCVQQSSGF TECRPYFTIL GLPAMLQAVR ALMIVGIVLG VIGILVSIFA LKCIRIGSMD DSAKAKMTLT SGILFIISGI CAIIGVSVFA NMLVTNFWMS TANMYSGMGG MGGMVQTVQT RYTFGAALFV GWVAGGLTLI GGVMMCIACR GLTPDDSNFK AVSYHASGQN VAYRPGGFKA STGFGSNTRN KKIYDGGART EDDEQSHPTK YDYV //