ID CXA2_CONMA STANDARD; PRT; 41 AA. AC P56636; DT 15-DEC-1998 (Rel. 37, Created) DT 15-JUN-2004 (Rel. 44, Last sequence update) DT 15-JUN-2004 (Rel. 44, Last annotation update) DE Alpha-conotoxin MII precursor (M2) (Fragment). OS Conus magus (Magus cone). OC Eukaryota; Metazoa; Mollusca; Gastropoda; Orthogastropoda; OC Apogastropoda; Caenogastropoda; Sorbeoconcha; Hypsogastropoda; OC Neogastropoda; Conoidea; Conidae; Conus. OX NCBI_TaxID=6492; RN [1] RP SEQUENCE FROM N.A. RC TISSUE=Hepatopancreas; RX MEDLINE=22206623; PubMed=12114524; RA McIntosh J.M., Dowell C., Watkins M., Garrett J.E., Yoshikami D., RA Olivera B.M.; RT "Alpha-conotoxin GIC from Conus geographus, a novel peptide RT antagonist of nicotinic acetylcholine receptors."; RL J. Biol. Chem. 277:33610-33615(2002). RN [2] RP SEQUENCE OF 22-37, AND SYNTHESIS. RC TISSUE=Venom; RX MEDLINE=96205934; PubMed=8631783; RA Cartier G.E., Yoshikami D., Gray W.R., Luo S., Olivera B.M., RA McIntosh J.M.; RT "A new alpha-conotoxin which targets alpha3beta2 nicotinic RT acetylcholine receptors."; RL J. Biol. Chem. 271:7522-7528(1996). RN [3] RP STRUCTURE BY NMR. RX MEDLINE=98062282; PubMed=9398298; RA Shon K.-J., Koerber S.C., Rivier J.E., Olivera B.M., McIntosh J.M.; RT "Three-dimensional solution structure of alpha-conotoxin MII, an RT alpha3beta2 neuronal nicotinic acetylcholine receptor-targeted RT ligand."; RL Biochemistry 36:15693-15700(1997). RN [4] RP STRUCTURE BY NMR. RX MEDLINE=99060038; PubMed=9843366; RA Hill J.M., Oomen C.J., Miranda L.P., Bingham J.-P., Alewood P.F., RA Craik D.J.; RT "Three-dimensional solution structure of alpha-conotoxin MII by NMR RT spectroscopy: effects of solution environment on helicity."; RL Biochemistry 37:15621-15630(1998). CC -!- FUNCTION: Alpha-conotoxins bind to the nicotinic acetylcholine CC receptors (nAChR) and inhibit them. In contrast to other alpha- CC conotoxins, this peptide has no detectable activity at the muscle CC subtype of receptor, but instead, it potently targets neuronal CC nAChR. It blocks alpha-3/beta-2 subunits of mammalian CC nAChR. It has an activity 2 to 4 orders of magnitude less potent CC on other nAChR subunit combinations. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- TISSUE SPECIFICITY: Expressed by the venom duct. CC -!- SIMILARITY: Belongs to the conotoxin A-superfamily. Alpha-type CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR PIR; A59046; A59046. DR PDB; 1M2C; 13-JAN-99. DR PDB; 1MII; 21-OCT-98. KW Toxin; Neurotoxin; Acetylcholine receptor inhibitor; Signal; KW Amidation; 3D-structure. FT NON_TER 1 1 FT SIGNAL <1 ? POTENTIAL. FT PROPEP ? 21 FT CHAIN 22 37 Alpha-conotoxin MII. FT DISULFID 23 29 FT DISULFID 24 37 FT MOD_RES 37 37 Cysteine amide (G-38 provides amide FT group). FT TURN 23 25 FT HELIX 28 32 FT TURN 33 37 SQ SEQUENCE 41 AA; 4354 MW; 6D2AF23E5F5A036D CRC64; SDGRNAAAND KASDVITLAL KGCCSNPVCH LEHSNLCGRR R //