ID   NDUV3_HUMAN             Reviewed;         108 AA.
AC   P56181; A8K0M1; J3KNX7; Q6FGD3; Q8WU60; Q9HCR5;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2002, sequence version 2.
DT   16-SEP-2015, entry version 123.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial;
DE   AltName: Full=Complex I-9kD;
DE            Short=CI-9kD;
DE   AltName: Full=NADH-ubiquinone oxidoreductase 9 kDa subunit;
DE   AltName: Full=Renal carcinoma antigen NY-REN-4;
DE   Flags: Precursor;
GN   Name=NDUFV3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9344673; DOI=10.1006/geno.1997.4930;
RA   de Coo R.F.M., Buddiger P., Smeets H.J.M., van Oost B.A.;
RT   "Molecular cloning and characterization of the human mitochondrial
RT   NADH:oxidoreductase 10-kDa gene (NDUFV3).";
RL   Genomics 45:434-437(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10950923; DOI=10.1006/geno.2000.6253;
RA   Berry A., Scott H.S., Kudoh J., Talior I., Korostishevsky M.,
RA   Wattenhofer M., Guipponi M., Barras C., Rossier C., Shibuya K.,
RA   Wang J., Kawasaki K., Asakawa S., Minoshima S., Shimizu N.,
RA   Antonarakis S.E., Bonne-Tamir B.;
RT   "Refined localization of autosomal recessive nonsyndromic deafness
RT   DFNB10 locus using 34 novel microsatellite markers, genomic structure,
RT   and exclusion of six known genes in the region.";
RL   Genomics 68:22-29(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
RA   Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
RA   Korn B., Zuo D., Hu Y., LaBaer J.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Cerebellum;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10830953; DOI=10.1038/35012518;
RA   Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T.,
RA   Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y.,
RA   Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K.,
RA   Polley A., Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D.,
RA   Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W.,
RA   Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S.,
RA   Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E.,
RA   Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P.,
RA   Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H.,
RA   Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E.,
RA   Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F.,
RA   Lehrach H., Reinhardt R., Yaspo M.-L.;
RT   "The DNA sequence of human chromosome 21.";
RL   Nature 405:311-319(2000).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain, and Uterus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   IDENTIFICATION AS A RENAL CANCER ANTIGEN.
RC   TISSUE=Renal cell carcinoma;
RX   PubMed=10508479;
RX   DOI=10.1002/(SICI)1097-0215(19991112)83:4<456::AID-IJC4>3.0.CO;2-5;
RA   Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H.,
RA   Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T.,
RA   Old L.J.;
RT   "Antigens recognized by autologous antibody in patients with renal-
RT   cell carcinoma.";
RL   Int. J. Cancer 83:456-464(1999).
RN   [9]
RP   IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=12611891; DOI=10.1074/jbc.C300064200;
RA   Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F.,
RA   Ghosh S.S., Capaldi R.A.;
RT   "The subunit composition of the human NADH dehydrogenase obtained by
RT   rapid one-step immunopurification.";
RL   J. Biol. Chem. 278:13619-13622(2003).
RN   [10]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA   Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA   Greff Z., Keri G., Stemmann O., Mann M.;
RT   "Kinase-selective enrichment enables quantitative phosphoproteomics of
RT   the kinome across the cell cycle.";
RL   Mol. Cell 31:438-448(2008).
RN   [11]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160; SER-162 AND SER-164
RP   (ISOFORM 2), AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP   ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [12]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
RN   [13]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S.,
RA   Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full
RT   phosphorylation site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
RN   [14]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA   Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J.,
RA   Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V.,
RA   Blagoev B.;
RT   "System-wide temporal characterization of the proteome and
RT   phosphoproteome of human embryonic stem cell differentiation.";
RL   Sci. Signal. 4:RS3-RS3(2011).
RN   [15]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162 AND SER-164 (ISOFORM
RP   2), AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D.,
RA   Wang L., Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human
RT   liver phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- FUNCTION: Accessory subunit of the mitochondrial membrane
CC       respiratory chain NADH dehydrogenase (Complex I), that is believed
CC       not to be involved in catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The
CC       immediate electron acceptor for the enzyme is believed to be
CC       ubiquinone.
CC   -!- SUBUNIT: Complex I is composed of 45 different subunits. This is a
CC       component of the flavoprotein-sulfur (FP) fragment of the enzyme.
CC       {ECO:0000269|PubMed:12611891}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral
CC       membrane protein; Matrix side.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P56181-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P56181-2; Sequence=VSP_038552;
CC         Note=Ref.7 (AAH21217) sequence is in conflict in position:
CC         415:D->N. Contains a phosphoserine at position 164. Contains a
CC         phosphoserine at position 160. Contains a phosphoserine at
CC         position 162. {ECO:0000244|PubMed:18669648,
CC         ECO:0000244|PubMed:24275569, ECO:0000305};
CC   -!- SIMILARITY: Belongs to the complex I NDUFV3 subunit family.
CC       {ECO:0000305}.
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DR   EMBL; X99726; CAB56704.1; -; Genomic_DNA.
DR   EMBL; X99727; CAB56704.1; JOINED; Genomic_DNA.
DR   EMBL; X99728; CAB56704.1; JOINED; Genomic_DNA.
DR   EMBL; AB038163; BAB13732.1; -; Genomic_DNA.
DR   EMBL; CR542174; CAG46971.1; -; mRNA.
DR   EMBL; CR542190; CAG46987.1; -; mRNA.
DR   EMBL; AK289586; BAF82275.1; -; mRNA.
DR   EMBL; AP001629; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471079; EAX09524.1; -; Genomic_DNA.
DR   EMBL; CH471079; EAX09526.1; -; Genomic_DNA.
DR   EMBL; BC021217; AAH21217.2; -; mRNA.
DR   EMBL; BC033766; AAH33766.1; -; mRNA.
DR   EMBL; BC054016; AAH54016.1; -; mRNA.
DR   CCDS; CCDS33572.1; -. [P56181-2]
DR   CCDS; CCDS33573.1; -. [P56181-1]
DR   RefSeq; NP_001001503.1; NM_001001503.1. [P56181-1]
DR   RefSeq; NP_066553.3; NM_021075.3. [P56181-2]
DR   UniGene; Hs.473937; -.
DR   ProteinModelPortal; P56181; -.
DR   BioGrid; 110809; 21.
DR   IntAct; P56181; 12.
DR   MINT; MINT-4535662; -.
DR   STRING; 9606.ENSP00000346196; -.
DR   ChEMBL; CHEMBL2363065; -.
DR   PhosphoSite; P56181; -.
DR   BioMuta; NDUFV3; -.
DR   MaxQB; P56181; -.
DR   PaxDb; P56181; -.
DR   PRIDE; P56181; -.
DR   DNASU; 4731; -.
DR   Ensembl; ENST00000340344; ENSP00000342895; ENSG00000160194. [P56181-1]
DR   Ensembl; ENST00000354250; ENSP00000346196; ENSG00000160194. [P56181-2]
DR   GeneID; 4731; -.
DR   KEGG; hsa:4731; -.
DR   UCSC; uc002zcm.3; human.
DR   UCSC; uc002zcn.3; human. [P56181-1]
DR   CTD; 4731; -.
DR   GeneCards; GC21P042879; -.
DR   HGNC; HGNC:7719; NDUFV3.
DR   HPA; CAB034142; -.
DR   HPA; HPA019791; -.
DR   HPA; HPA020463; -.
DR   HPA; HPA030427; -.
DR   MIM; 602184; gene.
DR   neXtProt; NX_P56181; -.
DR   PharmGKB; PA31529; -.
DR   eggNOG; NOG47709; -.
DR   GeneTree; ENSGT00390000012196; -.
DR   HOGENOM; HOG000013001; -.
DR   HOVERGEN; HBG082013; -.
DR   InParanoid; P56181; -.
DR   KO; K03944; -.
DR   OMA; SQKPFEV; -.
DR   OrthoDB; EOG7QZGCS; -.
DR   PhylomeDB; P56181; -.
DR   TreeFam; TF338771; -.
DR   Reactome; R-HSA-611105; Respiratory electron transport.
DR   ChiTaRS; NDUFV3; human.
DR   GeneWiki; NDUFV3; -.
DR   GenomeRNAi; 4731; -.
DR   NextBio; 18240; -.
DR   PRO; PR:P56181; -.
DR   Proteomes; UP000005640; Chromosome 21.
DR   Bgee; P56181; -.
DR   CleanEx; HS_NDUFV3; -.
DR   Genevisible; P56181; HS.
DR   GO; GO:0005743; C:mitochondrial inner membrane; TAS:Reactome.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; NAS:UniProtKB.
DR   GO; GO:0044822; F:poly(A) RNA binding; IDA:UniProtKB.
DR   GO; GO:0044237; P:cellular metabolic process; TAS:Reactome.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; NAS:UniProtKB.
DR   GO; GO:0022904; P:respiratory electron transport chain; TAS:Reactome.
DR   GO; GO:0044281; P:small molecule metabolic process; TAS:Reactome.
DR   InterPro; IPR026193; NDUFV3.
DR   PANTHER; PTHR17117; PTHR17117; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; Electron transport; Membrane;
KW   Mitochondrion; Mitochondrion inner membrane; Phosphoprotein;
KW   Reference proteome; Respiratory chain; Transit peptide; Transport.
FT   TRANSIT       1     34       Mitochondrion. {ECO:0000250}.
FT   CHAIN        35    108       NADH dehydrogenase [ubiquinone]
FT                                flavoprotein 3, mitochondrial.
FT                                /FTId=PRO_0000020025.
FT   VAR_SEQ      56     57       KK -> KNVVEPKERGKLLATQTAAELSKNLSSPSSYPPAV
FT                                NKGRKVASPSPSGSVLFTDEGVPKFLSRKTLVEFPQKVLSP
FT                                FRKQGSDSEARQVGRKVTSPSSSSSSSSSDSESDDEADVSE
FT                                VTPRVVSKGRGGLRKPEASHSFENRAPRVTVSAKEKTLLQK
FT                                PHVDITDPEKPHQPKKKGSPAKPSEGRENARPKTTMPRSQV
FT                                DEEFLKQSLKEKQLQKTFRLNEIDKESQKPFEVKGPLPVHT
FT                                KSGLSAPPKGSPAPAVLAEEARAEGQLQASPPGAAEGHLEK
FT                                PVPEPQRKAAPPLPRKETSGTQGIEGHLKGGQAIVEDQIPP
FT                                SNLETVPVENNHGFHEKTAALKLEAEGEAMEDAAAPGDDRG
FT                                GTQE (in isoform 2).
FT                                {ECO:0000303|PubMed:15489334}.
FT                                /FTId=VSP_038552.
FT   CONFLICT      9      9       Q -> K (in Ref. 1; CAB56704).
FT                                {ECO:0000305}.
SQ   SEQUENCE   108 AA;  11941 MW;  BD7375474AB0B1EC CRC64;
     MAAPCLLRQG RAGALKTMLQ EAQVFRGLAS TVSLSAESGK SEKGQPQNSK KQSPPKKPAP
     VPAEPFDNTT YKNLQHHDYS TYTFLDLNLE LSKFRMPQPS SGRESPRH
//