ID PNPH_BOVIN Reviewed; 289 AA. AC P55859; Q58DQ2; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 15-JUL-1998, sequence version 2. DT 23-OCT-2007, entry version 57. DE Purine nucleoside phosphorylase (EC 2.4.2.1) (Inosine phosphorylase) DE (PNP). GN Name=NP; Synonyms=PNP; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; OC Pecora; Bovidae; Bovinae; Bos. OX NCBI_TaxID=9913; RN [1] RP PROTEIN SEQUENCE. RC TISSUE=Spleen; RX MEDLINE=95331377; PubMed=7607309; DOI=10.1016/0014-5793(95)00540-P; RA Bzowska A., Luic M., Schroeder W., Shugar D., Saenger W., Koellner G.; RT "Calf spleen purine nucleoside phosphorylase: purification, sequence RT and crystal structure of its complex with an N(7)-acycloguanosine RT inhibitor."; RL FEBS Lett. 367:214-218(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=16305752; DOI=10.1186/1471-2164-6-166; RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.; RT "Characterization of 954 bovine full-CDS cDNA sequences."; RL BMC Genomics 6:166-166(2005). RN [3] RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). RC TISSUE=Spleen; RX MEDLINE=97173115; PubMed=9020983; DOI=10.1006/jmbi.1996.0730; RA Koellner G., Luic M., Shugar D., Saenger W., Bzowska A.; RT "Crystal structure of calf spleen purine nucleoside phosphorylase in a RT complex with hypoxanthine at 2.15-A resolution."; RL J. Mol. Biol. 265:202-216(1997). RN [4] RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). RA Mao C., Zhou M., Ealick S., Federov A.A., Almo S.C.; RL Submitted (JUL-1995) to the PDB data bank. RN [5] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). RC TISSUE=Spleen; RX MEDLINE=98254498; PubMed=9585525; DOI=10.1021/bi9723919; RA Mao C., Cook W.J., Zhou M., Federov A.A., Almo S.C., Ealick S.E.; RT "Calf spleen purine nucleoside phosphorylase complexed with substrates RT and substrate analogues."; RL Biochemistry 37:7135-7146(1998). RN [6] RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). RC TISSUE=Spleen; RA Pugmire M.J., Mao C., Ealick S.E.; RT "The high resolution crystal structure of bovine spleen purine RT nucleoside phosphorylase in complex forms with phosphate and 9- RT deazainosine."; RL Submitted (MAR-1998) to the PDB data bank. CC -!- CATALYTIC ACTIVITY: Purine nucleoside + phosphate = purine + CC alpha-D-ribose 1-phosphate. CC -!- SUBUNIT: Homotrimer. CC -!- SIMILARITY: Belongs to the PNP/MTAP phosphorylase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BT021545; AAX46392.1; -; mRNA. DR PIR; S66203; S66203. DR UniGene; Bt.65253; -. DR PDB; 1A9O; X-ray; @=1-289. DR PDB; 1A9P; X-ray; @=1-289. DR PDB; 1A9Q; X-ray; @=1-282. DR PDB; 1A9R; X-ray; @=1-282. DR PDB; 1A9S; X-ray; @=1-289. DR PDB; 1A9T; X-ray; @=1-284. DR PDB; 1B8N; X-ray; A=1-284. DR PDB; 1B8O; X-ray; A=1-284. DR PDB; 1FXU; X-ray; A=1-289. DR PDB; 1LV8; X-ray; A/B/C/D/E/F=1-289. DR PDB; 1LVU; X-ray; A/B/C/D/E/F=1-289. DR PDB; 1PBN; X-ray; @=1-289. DR PDB; 1V48; X-ray; A=1-289. DR PDB; 1VFN; X-ray; @=4-284. DR PDB; 2AI1; X-ray; A=1-289. DR PDB; 2AI2; X-ray; A=1-289. DR PDB; 2AI3; X-ray; A=1-289. DR PDB; 3PNP; X-ray; @=1-289. DR PDB; 4PNP; X-ray; @=1-289. DR Ensembl; ENSBTAG00000012317; Bos taurus. DR KEGG; bta:787045; -. DR LinkHub; P55859; -. DR InterPro; IPR001369; Mtap_PNP. DR InterPro; IPR011270; PNPH. DR InterPro; IPR011268; PNPHPUNA_XAPA. DR PANTHER; PTHR11904; Mtap_PNP; 1. DR Pfam; PF00896; Mtap_PNP; 1. DR PIRSF; PIRSF000477; PurNPase; 1. DR TIGRFAMs; TIGR01700; PNPH; 1. DR TIGRFAMs; TIGR01697; PNPH-PUNA-XAPA; 1. DR PROSITE; PS01240; PNP_MTAP_2; 1. PE 1: Evidence at protein level; KW 3D-structure; Acetylation; Direct protein sequencing; KW Glycosyltransferase; Transferase. FT CHAIN 1 289 Purine nucleoside phosphorylase. FT /FTId=PRO_0000184535. FT MOD_RES 1 1 N-acetylmethionine (By similarity). FT CONFLICT 2 2 Q -> A (in Ref. 2). FT CONFLICT 25 25 P -> S (in Ref. 2). FT HELIX 7 18 FT STRAND 26 31 FT HELIX 33 41 FT STRAND 43 49 FT TURN 50 52 FT STRAND 62 64 FT STRAND 67 73 FT STRAND 76 83 FT HELIX 87 89 FT HELIX 93 96 FT HELIX 98 105 FT STRAND 110 119 FT STRAND 129 137 FT HELIX 138 141 FT TURN 153 155 FT HELIX 168 180 FT STRAND 188 194 FT HELIX 203 211 FT STRAND 215 221 FT HELIX 222 230 FT STRAND 234 244 FT STRAND 248 250 FT HELIX 257 260 FT TURN 261 265 FT HELIX 266 278 FT TURN 279 281 FT STRAND 284 287 SQ SEQUENCE 289 AA; 32094 MW; 62D243C3016E8EE9 CRC64; MQNGYTYEDY QDTAKWLLSH TEQRPQVAVI CGSGLGGLVN KLTQAQTFDY SEIPNFPEST VPGHAGRLVF GILNGRACVM MQGRFHMYEG YPFWKVTFPV RVFRLLGVET LVVTNAAGGL NPNFEVGDIM LIRDHINLPG FSGENPLRGP NEERFGVRFP AMSDAYDRDM RQKAHSTWKQ MGEQRELQEG TYVMLGGPNF ETVAECRLLR NLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SQGKANHEEV LEAGKQAAQK LEQFVSLLMA SIPVSGHTG //