ID PNPH_BOVIN STANDARD; PRT; 289 AA. AC P55859; DT 01-NOV-1997 (Rel. 35, Created) DT 15-JUL-1998 (Rel. 36, Last sequence update) DT 15-JUL-1999 (Rel. 38, Last annotation update) DE PURINE NUCLEOSIDE PHOSPHORYLASE (EC 2.4.2.1) (INOSINE PHOSPHORYLASE) DE (PNP). GN NP. OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia; OC Eutheria; Cetartiodactyla; Ruminantia; Pecora; Bovoidea; Bovidae; OC Bovinae; Bos. RN [1] RP SEQUENCE. RC TISSUE=SPLEEN; RX MEDLINE; 95331377. RA BZOWSKA A., LUIC M., SCHRODER W., SHUGAR D., SAENGER W., KOELLNER G.; RT "Calf spleen purine nucleoside phosphorylase: purification, sequence RT and crystal structure of its complex with an N(7)-acycloguanosine RT inhibitor."; RL FEBS Lett. 367:214-218(1995). RN [2] RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). RC TISSUE=SPLEEN; RX MEDLINE; 97173115. RA KOELLNER G., LUIC M., SHUGAR D., SAENGER W., BZOWSKA A.; RT "Crystal structure of calf spleen purine nucleoside phosphorylase in RT a complex with hypoxanthine at 2.15-A resolution."; RL J. Mol. Biol. 265:202-216(1997). RN [3] RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). RA MAO C., ZHOU M., EALICK S., FEDEROV A.A., ALMO S.C.; RL Submitted (JUL-1995) to the PDB data bank. RN [4] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). RC TISSUE=SPLEEN; RX MEDLINE; 98254498. RA MAO C., COOK W.J., ZHOU M., FEDEROV A.A., ALMO S.C., EALICK S.E.; RT "Calf spleen purine nucleoside phosphorylase complexed with RT substrates and substrate analogues."; RL Biochemistry 37:7135-7146(1998). RN [5] RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). RC TISSUE=SPLEEN; RA PUGMIRE M.J., MAO C., EALICK S.E.; RT "The high resolution crystal structure of bovine spleen purine RT nucleoside phosphorylase in complex forms with phosphate and 9- RT deazainosine."; RL Submitted (MAR-1998) to the PDB data bank. CC -!- CATALYTIC ACTIVITY: PURINE NUCLEOSIDE + ORTHOPHOSPHATE = PURINE + CC ALPHA-D-RIBOSE 1-PHOSPHATE. CC -!- SUBUNIT: HOMOTRIMER. CC -!- SIMILARITY: BELONGS TO THE PNP/MTAP FAMILY 2 OF PHOSPHORYLASES. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR PDB; 1VFN; 12-NOV-97. DR PDB; 1PBN; 14-NOV-95. DR PDB; 1A9O; 15-JUL-98. DR PDB; 1A9P; 15-JUL-98. DR PDB; 1A9Q; 15-JUL-98. DR PDB; 1A9R; 15-JUL-98. DR PDB; 1A9S; 15-JUL-98. DR PDB; 1A9T; 17-JUN-98. DR PDB; 1B8N; 16-FEB-99. DR PDB; 1B8O; 16-FEB-99. DR PDB; 3PNP; 30-MAR-99. DR PDB; 4PNP; 30-MAR-99. DR PFAM; PF00896; Mtap_PNP; 1. DR PROSITE; PS01240; PNP_MTAP_2; 1. KW Transferase; Glycosyltransferase; 3D-structure. SQ SEQUENCE 289 AA; 32093 MW; 550EF6A0 CRC32; MQNGYTYEDY QDTAKWLLSH TEQRPQVAVI CGSGLGGLVN KLTQAQTFDY SEIPNFPEST VPGHAGRLVF GILNGRACVM MQGRFHMYEG YPFWKVTFPV RVFRLLGVET LVVTNAAGGL NPNFEVGDIM LIRDHINLPG FSGENPLRGP NEERFGVRFP AMSDAYDRDM RQKAHSTWKQ MGEQRELQEG TYVMLGGPNF ETVAECRLLR NLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SQGKANHEEV LEAGKQAAQK LEQFVSLLMA SIPVSGHTG //