ID PNPH_BOVIN STANDARD; PRT; 289 AA. AC P55859; Q58DQ2; DT 01-NOV-1997 (Rel. 35, Created) DT 15-JUL-1998 (Rel. 36, Last sequence update) DT 13-SEP-2005 (Rel. 48, Last annotation update) DE Purine nucleoside phosphorylase (EC 2.4.2.1) (Inosine phosphorylase) DE (PNP). GN Name=NP; Synonyms=PNP; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; OC Pecora; Bovidae; Bovinae; Bos. OX NCBI_TaxID=9913; RN [1] RP PROTEIN SEQUENCE. RC TISSUE=Spleen; RX MEDLINE=95331377; PubMed=7607309; DOI=10.1016/0014-5793(95)00540-P; RA Bzowska A., Luic M., Schroeder W., Shugar D., Saenger W., Koellner G.; RT "Calf spleen purine nucleoside phosphorylase: purification, sequence RT and crystal structure of its complex with an N(7)-acycloguanosine RT inhibitor."; RL FEBS Lett. 367:214-218(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Harhay G.P., Sonstegard T.S., Van Tassell C.P., Clawson M.L., RA Heaton M.P., Keele J.W., Snelling W.M., Weidmann R.T., Smith T.P.L.; RT "Sequencing and analysis of Bos taurus full-length insert cDNA RT clones."; RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases. RN [3] RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). RC TISSUE=Spleen; RX MEDLINE=97173115; PubMed=9020983; DOI=10.1006/jmbi.1996.0730; RA Koellner G., Luic M., Shugar D., Saenger W., Bzowska A.; RT "Crystal structure of calf spleen purine nucleoside phosphorylase in a RT complex with hypoxanthine at 2.15-A resolution."; RL J. Mol. Biol. 265:202-216(1997). RN [4] RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). RA Mao C., Zhou M., Ealick S., Federov A.A., Almo S.C.; RL Submitted (JUL-1995) to the PDB data bank. RN [5] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). RC TISSUE=Spleen; RX MEDLINE=98254498; PubMed=9585525; DOI=10.1021/bi9723919; RA Mao C., Cook W.J., Zhou M., Federov A.A., Almo S.C., Ealick S.E.; RT "Calf spleen purine nucleoside phosphorylase complexed with substrates RT and substrate analogues."; RL Biochemistry 37:7135-7146(1998). RN [6] RP X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS). RC TISSUE=Spleen; RA Pugmire M.J., Mao C., Ealick S.E.; RT "The high resolution crystal structure of bovine spleen purine RT nucleoside phosphorylase in complex forms with phosphate and 9- RT deazainosine."; RL Submitted (MAR-1998) to the PDB data bank. CC -!- CATALYTIC ACTIVITY: Purine nucleoside + phosphate = purine + CC alpha-D-ribose 1-phosphate. CC -!- SUBUNIT: Homotrimer. CC -!- SIMILARITY: Belongs to the PNP/MTAP phosphorylase family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BT021545; AAX46392.1; -; mRNA. DR PDB; 1A9O; X-ray; @=1-289. DR PDB; 1A9P; X-ray; @=1-289. DR PDB; 1A9Q; X-ray; @=1-282. DR PDB; 1A9R; X-ray; @=1-282. DR PDB; 1A9S; X-ray; @=1-289. DR PDB; 1A9T; X-ray; @=3-284. DR PDB; 1B8N; X-ray; A=1-284. DR PDB; 1B8O; X-ray; A=1-284. DR PDB; 1FXU; X-ray; A=1-289. DR PDB; 1LV8; X-ray; A/B/C/D/E/F=1-289. DR PDB; 1LVU; X-ray; A/B/C/D/E/F=1-289. DR PDB; 1PBN; X-ray; @=3-289. DR PDB; 1V48; X-ray; A=1-289. DR PDB; 1VFN; X-ray; @=4-284. DR PDB; 3PNP; X-ray; @=3-289. DR PDB; 4PNP; X-ray; @=3-289. DR LinkHub; P55859; -. DR InterPro; IPR001369; Mtap_PNP. DR InterPro; IPR011270; PNPH. DR InterPro; IPR011268; PNPHPUNA_XAPA. DR PANTHER; PTHR11904; Mtap_PNP; 1. DR PANTHER; PTHR11904:SF3; PNPH; 1. DR Pfam; PF00896; Mtap_PNP; 1. DR PIRSF; PIRSF000477; PurNPase; 1. DR TIGRFAMs; TIGR01700; PNPH; 1. DR TIGRFAMs; TIGR01697; PNPH-PUNA-XAPA; 1. DR PROSITE; PS01240; PNP_MTAP_2; 1. KW 3D-structure; Direct protein sequencing; Glycosyltransferase; KW Transferase. FT CONFLICT 2 2 Q -> A (in Ref. 2). FT CONFLICT 25 25 P -> S (in Ref. 2). FT HELIX 7 20 FT STRAND 27 31 FT TURN 33 34 FT HELIX 36 39 FT TURN 40 41 FT STRAND 43 49 FT HELIX 50 52 FT TURN 54 55 FT TURN 62 63 FT STRAND 67 73 FT TURN 74 75 FT STRAND 76 82 FT HELIX 87 89 FT TURN 90 90 FT HELIX 93 96 FT TURN 97 97 FT HELIX 98 106 FT TURN 107 107 FT STRAND 110 119 FT TURN 122 123 FT TURN 126 127 FT STRAND 129 137 FT HELIX 138 141 FT TURN 142 143 FT TURN 146 147 FT TURN 153 155 FT TURN 163 164 FT STRAND 166 166 FT HELIX 168 180 FT TURN 181 182 FT STRAND 188 194 FT HELIX 203 211 FT TURN 212 213 FT STRAND 216 218 FT HELIX 222 230 FT TURN 231 232 FT STRAND 234 244 FT TURN 249 250 FT HELIX 257 279 FT TURN 280 281 SQ SEQUENCE 289 AA; 32094 MW; 62D243C3016E8EE9 CRC64; MQNGYTYEDY QDTAKWLLSH TEQRPQVAVI CGSGLGGLVN KLTQAQTFDY SEIPNFPEST VPGHAGRLVF GILNGRACVM MQGRFHMYEG YPFWKVTFPV RVFRLLGVET LVVTNAAGGL NPNFEVGDIM LIRDHINLPG FSGENPLRGP NEERFGVRFP AMSDAYDRDM RQKAHSTWKQ MGEQRELQEG TYVMLGGPNF ETVAECRLLR NLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SQGKANHEEV LEAGKQAAQK LEQFVSLLMA SIPVSGHTG //