ID   SNAA_HUMAN              Reviewed;         295 AA.
AC   P54920; A8K879; Q96IK3; Q9BVJ3;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 3.
DT   27-MAY-2015, entry version 142.
DE   RecName: Full=Alpha-soluble NSF attachment protein;
DE            Short=SNAP-alpha;
DE   AltName: Full=N-ethylmaleimide-sensitive factor attachment protein alpha;
GN   Name=NAPA; Synonyms=SNAPA;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Platelet;
RX   PubMed=9269766;
RA   Lemons P.P., Chen D., Bernstein A.M., Bennett M.K., Whiteheart S.W.;
RT   "Regulated secretion in platelets: identification of elements of the
RT   platelet exocytosis machinery.";
RL   Blood 90:1490-1500(1997).
RN   [2]
RP   SEQUENCE REVISION TO 91; 133 AND 289.
RA   Chen D., Shao H.P., Whiteheart S.W.;
RL   Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA   Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA   Phelan M., Farmer A.;
RT   "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT   vector.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain, Lung, and Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN A COMPLEX
RP   WITH RINT1; USE1L; NAPA AND ZW10.
RX   PubMed=15029241; DOI=10.1038/sj.emboj.7600135;
RA   Hirose H., Arasaki K., Dohmae N., Takio K., Hatsuzawa K., Nagahama M.,
RA   Tani K., Yamamoto A., Tohyama M., Tagaya M.;
RT   "Implication of ZW10 in membrane trafficking between the endoplasmic
RT   reticulum and Golgi.";
RL   EMBO J. 23:1267-1278(2004).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [9]
RP   ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E.,
RA   Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K.,
RA   Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-
RT   terminal acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-195, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D.,
RA   Wang L., Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human
RT   liver phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
CC   -!- FUNCTION: Required for vesicular transport between the endoplasmic
CC       reticulum and the Golgi apparatus.
CC   -!- SUBUNIT: Interacts with PRKCABP, and disrupts the interaction
CC       between GRIA2 and PRKCABP, leading to the internalization of
CC       GRIA2. Found in a complex with VAMP8 (By similarity). Component of
CC       a SNARE-like complex that contains at least ZW10, USE1L, RINT1,
CC       STX18 and NAPA/SNAP-alpha. Interacts with VTI1A. Interacts with
CC       STX12 (By similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       P46459:NSF; NbExp=2; IntAct=EBI-749652, EBI-712251;
CC       O00161:SNAP23; NbExp=2; IntAct=EBI-749652, EBI-745000;
CC       Q12846:STX4; NbExp=2; IntAct=EBI-749652, EBI-744942;
CC   -!- SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the SNAP family. {ECO:0000305}.
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DR   EMBL; U39412; AAC80170.1; -; mRNA.
DR   EMBL; BT019568; AAV38375.1; -; mRNA.
DR   EMBL; AK292244; BAF84933.1; -; mRNA.
DR   EMBL; CH471126; EAW57491.1; -; Genomic_DNA.
DR   EMBL; BC001165; AAH01165.1; -; mRNA.
DR   EMBL; BC007432; AAH07432.1; -; mRNA.
DR   EMBL; BC028234; AAH28234.1; -; mRNA.
DR   EMBL; BC091511; AAH91511.1; -; mRNA.
DR   CCDS; CCDS12702.1; -.
DR   PIR; G02238; G02238.
DR   RefSeq; NP_003818.2; NM_003827.3.
DR   UniGene; Hs.730799; -.
DR   UniGene; Hs.744881; -.
DR   ProteinModelPortal; P54920; -.
DR   SMR; P54920; 19-293.
DR   BioGrid; 114305; 35.
DR   DIP; DIP-393N; -.
DR   IntAct; P54920; 8.
DR   MINT; MINT-3020680; -.
DR   STRING; 9606.ENSP00000263354; -.
DR   PhosphoSite; P54920; -.
DR   BioMuta; NAPA; -.
DR   DMDM; 116242794; -.
DR   OGP; P54920; -.
DR   MaxQB; P54920; -.
DR   PaxDb; P54920; -.
DR   PeptideAtlas; P54920; -.
DR   PRIDE; P54920; -.
DR   DNASU; 8775; -.
DR   Ensembl; ENST00000263354; ENSP00000263354; ENSG00000105402.
DR   GeneID; 8775; -.
DR   KEGG; hsa:8775; -.
DR   UCSC; uc002pha.2; human.
DR   CTD; 8775; -.
DR   GeneCards; GC19M047990; -.
DR   H-InvDB; HIX0137469; -.
DR   HGNC; HGNC:7641; NAPA.
DR   HPA; CAB010100; -.
DR   HPA; HPA046149; -.
DR   HPA; HPA050196; -.
DR   MIM; 603215; gene.
DR   neXtProt; NX_P54920; -.
DR   PharmGKB; PA31443; -.
DR   eggNOG; NOG245914; -.
DR   GeneTree; ENSGT00390000005826; -.
DR   HOGENOM; HOG000165015; -.
DR   HOVERGEN; HBG001325; -.
DR   InParanoid; P54920; -.
DR   KO; K15296; -.
DR   OMA; MQSKHDA; -.
DR   OrthoDB; EOG7FNC80; -.
DR   PhylomeDB; P54920; -.
DR   TreeFam; TF316547; -.
DR   Reactome; REACT_19400; Golgi Associated Vesicle Biogenesis.
DR   ChiTaRS; NAPA; human.
DR   GeneWiki; NAPA_(gene); -.
DR   GenomeRNAi; 8775; -.
DR   NextBio; 32904; -.
DR   PMAP-CutDB; P54920; -.
DR   PRO; PR:P54920; -.
DR   Proteomes; UP000005640; Chromosome 19.
DR   Bgee; P54920; -.
DR   CleanEx; HS_NAPA; -.
DR   ExpressionAtlas; P54920; baseline and differential.
DR   Genevestigator; P54920; -.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0070062; C:extracellular exosome; IDA:UniProtKB.
DR   GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR   GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR   GO; GO:0070044; C:synaptobrevin 2-SNAP-25-syntaxin-1a complex; IEA:Ensembl.
DR   GO; GO:0005774; C:vacuolar membrane; IBA:GO_Central.
DR   GO; GO:0005483; F:soluble NSF attachment protein activity; IBA:GO_Central.
DR   GO; GO:0019905; F:syntaxin binding; IBA:GO_Central.
DR   GO; GO:0045176; P:apical protein localization; IEA:Ensembl.
DR   GO; GO:0007420; P:brain development; IEA:Ensembl.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; TAS:ProtInc.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0061025; P:membrane fusion; IBA:GO_Central.
DR   GO; GO:0061024; P:membrane organization; TAS:Reactome.
DR   GO; GO:0030182; P:neuron differentiation; IEA:Ensembl.
DR   GO; GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.
DR   GO; GO:0010807; P:regulation of synaptic vesicle priming; IEA:Ensembl.
DR   GO; GO:0035494; P:SNARE complex disassembly; IBA:GO_Central.
DR   GO; GO:0035249; P:synaptic transmission, glutamatergic; IEA:Ensembl.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR000744; NSF_attach.
DR   InterPro; IPR011990; TPR-like_helical_dom.
DR   PANTHER; PTHR13768; PTHR13768; 1.
DR   PRINTS; PR00448; NSFATTACHMNT.
PE   1: Evidence at protein level;
KW   Acetylation; Complete proteome; ER-Golgi transport; Membrane;
KW   Phosphoprotein; Protein transport; Reference proteome; Transport.
FT   CHAIN         1    295       Alpha-soluble NSF attachment protein.
FT                                /FTId=PRO_0000219056.
FT   MOD_RES       1      1       N-acetylmethionine.
FT                                {ECO:0000269|PubMed:22814378}.
FT   MOD_RES     195    195       Phosphoserine.
FT                                {ECO:0000269|PubMed:24275569}.
FT   CONFLICT    195    195       S -> T (in Ref. 1; AAC80170).
FT                                {ECO:0000305}.
SQ   SEQUENCE   295 AA;  33233 MW;  7704A619A441E609 CRC64;
     MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA
     AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA
     AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK
     AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE
     CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
//