ID ACT4_ARATH Reviewed; 377 AA. AC P53494; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 1. DT 26-FEB-2008, entry version 58. DE Actin-4. GN Name=ACT4; OrderedLocusNames=At5g59370; ORFNames=F2O15.3; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION. RC STRAIN=cv. Columbia; RX MEDLINE=96367663; PubMed=8771777; RA Huang S., An Y.-Q., McDowell J.M., McKinney E.C., Meagher R.B.; RT "The Arabidopsis thaliana ACT4/ACT12 actin gene subclass is strongly RT expressed throughout pollen development."; RL Plant J. 10:189-202(1996). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., RA Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI."; RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [4] RP GENE FAMILY ORGANIZATION, AND CHARACTERIZATION. RC STRAIN=cv. Columbia; RX MEDLINE=97005557; PubMed=8852856; RA McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.; RT "Structure and evolution of the actin gene family in Arabidopsis RT thaliana."; RL Genetics 142:587-602(1996). CC -!- FUNCTION: Actins are highly conserved proteins that are involved CC in various types of cell motility and are ubiquitously expressed CC in all eukaryotic cells. Essential component of cell cytoskeleton; CC plays an important role in cytoplasmic streaming, cell shape CC determination, cell division, organelle movement and extension CC growth. This is considered as one of the reproductive actins. CC -!- SUBUNIT: Polymerization of globular actin (G-actin) leads to a CC structural filament (F-actin) in the form of a two-stranded helix. CC The binding of profilin to monomeric G-actin cause the CC sequestration of actin into profilactin complexes, and prevents CC the polymerization. CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. CC -!- TISSUE SPECIFICITY: Little or no reproductive-gene expression is CC detected in vegetative organs, such as root, stems, leaves, sepals CC and petals. CC -!- DEVELOPMENTAL STAGE: Expressed primarily in pollen. CC -!- MISCELLANEOUS: There are 8 actin genes in A.thaliana. CC -!- SIMILARITY: Belongs to the actin family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U27980; AAB39403.1; -; Genomic_DNA. DR EMBL; AB025604; BAA97473.1; -; Genomic_DNA. DR EMBL; AY088111; AAM65657.1; -; mRNA. DR PIR; S68108; S68108. DR RefSeq; NP_001078769.1; -. DR RefSeq; NP_200745.1; -. DR UniGene; At.29229; -. DR HSSP; P02568; 1MA9. DR SMR; P53494; 10-377. DR GeneID; 836056; -. DR GenomeReviews; BA000015_GR; AT5G59370. DR KEGG; ath:AT5G59370; -. DR TAIR; At5g59370; -. DR GermOnline; AT5G59370; Arabidopsis thaliana. DR InterPro; IPR004001; Actin_CS. DR InterPro; IPR004000; Actin_like. DR PANTHER; PTHR11937; Actin_like; 1. DR Pfam; PF00022; Actin; 1. DR PRINTS; PR00190; ACTIN. DR SMART; SM00268; ACTIN; 1. DR PROSITE; PS00406; ACTINS_1; 1. DR PROSITE; PS00432; ACTINS_2; 1. DR PROSITE; PS01132; ACTINS_ACT_LIKE; 1. PE 1: Evidence at protein level; KW ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; KW Nucleotide-binding; Structural protein. FT CHAIN 1 377 Actin-4. FT /FTId=PRO_0000088890. FT CONFLICT 154 154 V -> F (in Ref. 3). SQ SEQUENCE 377 AA; 41779 MW; 3BC5B633D2BB088A CRC64; MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP EEHPVLLTEA PLNPKANREK MTQIMFETFN TPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDH LMKILTERGY SFTTTAEREI VRDMKEKLSY IALDFEQELE TSKTSSSVEK SFELPDGQVI TIGAERFRCP EVLFQPSMIG MENPGIHETT YNSIMKCDVD IRKDLYGNIV LSGGTTMFGG IGDRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIA KAEYDESGPS IVHRKCF //