ID ACT4_ARATH Reviewed; 377 AA. AC P53494; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 1. DT 03-AUG-2022, entry version 138. DE RecName: Full=Actin-4; GN Name=ACT4; OrderedLocusNames=At5g59370; ORFNames=F2O15.3; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION. RC STRAIN=cv. Columbia; RX PubMed=8771777; DOI=10.1046/j.1365-313x.1996.10020189.x; RA Huang S., An Y.-Q., McDowell J.M., McKinney E.C., Meagher R.B.; RT "The Arabidopsis thaliana ACT4/ACT12 actin gene subclass is strongly RT expressed throughout pollen development."; RL Plant J. 10:189-202(1996). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., RA Tabata S.; RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI."; RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases. RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [5] RP GENE FAMILY ORGANIZATION, AND CHARACTERIZATION. RC STRAIN=cv. Columbia; RX PubMed=8852856; DOI=10.1093/genetics/142.2.587; RA McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.; RT "Structure and evolution of the actin gene family in Arabidopsis RT thaliana."; RL Genetics 142:587-602(1996). CC -!- FUNCTION: Actins are highly conserved proteins that are involved in CC various types of cell motility and are ubiquitously expressed in all CC eukaryotic cells. Essential component of cell cytoskeleton; plays an CC important role in cytoplasmic streaming, cell shape determination, cell CC division, organelle movement and extension growth. This is considered CC as one of the reproductive actins. CC -!- SUBUNIT: Polymerization of globular actin (G-actin) leads to a CC structural filament (F-actin) in the form of a two-stranded helix. The CC binding of profilin to monomeric G-actin cause the sequestration of CC actin into profilactin complexes, and prevents the polymerization. CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. CC -!- TISSUE SPECIFICITY: Little or no reproductive-gene expression is CC detected in vegetative organs, such as root, stems, leaves, sepals and CC petals. CC -!- DEVELOPMENTAL STAGE: Expressed primarily in pollen. CC -!- MISCELLANEOUS: There are 8 actin genes in A.thaliana. CC -!- SIMILARITY: Belongs to the actin family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U27980; AAB39403.1; -; Genomic_DNA. DR EMBL; AB025604; BAA97473.1; -; Genomic_DNA. DR EMBL; CP002688; AED97176.1; -; Genomic_DNA. DR EMBL; CP002688; AED97177.1; -; Genomic_DNA. DR EMBL; AY088111; AAM65657.1; -; mRNA. DR PIR; S68108; S68108. DR RefSeq; NP_001078769.1; NM_001085300.1. DR RefSeq; NP_200745.1; NM_125328.4. DR AlphaFoldDB; P53494; -. DR SMR; P53494; -. DR STRING; 3702.AT5G59370.2; -. DR PaxDb; P53494; -. DR PRIDE; P53494; -. DR ProteomicsDB; 244794; -. DR EnsemblPlants; AT5G59370.1; AT5G59370.1; AT5G59370. DR EnsemblPlants; AT5G59370.2; AT5G59370.2; AT5G59370. DR GeneID; 836056; -. DR Gramene; AT5G59370.1; AT5G59370.1; AT5G59370. DR Gramene; AT5G59370.2; AT5G59370.2; AT5G59370. DR KEGG; ath:AT5G59370; -. DR Araport; AT5G59370; -. DR TAIR; locus:2148298; AT5G59370. DR eggNOG; KOG0676; Eukaryota. DR HOGENOM; CLU_027965_0_2_1; -. DR InParanoid; P53494; -. DR OMA; GRCCELK; -. DR OrthoDB; 649708at2759; -. DR PhylomeDB; P53494; -. DR PRO; PR:P53494; -. DR Proteomes; UP000006548; Chromosome 5. DR ExpressionAtlas; P53494; baseline and differential. DR Genevisible; P53494; AT. DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell. DR GO; GO:0005739; C:mitochondrion; HDA:TAIR. DR GO; GO:0009536; C:plastid; HDA:TAIR. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0005200; F:structural constituent of cytoskeleton; ISS:TAIR. DR InterPro; IPR004000; Actin. DR InterPro; IPR020902; Actin/actin-like_CS. DR InterPro; IPR004001; Actin_CS. DR InterPro; IPR043129; ATPase_NBD. DR PANTHER; PTHR11937; PTHR11937; 1. DR Pfam; PF00022; Actin; 1. DR PRINTS; PR00190; ACTIN. DR SMART; SM00268; ACTIN; 1. DR SUPFAM; SSF53067; SSF53067; 2. DR PROSITE; PS00406; ACTINS_1; 1. DR PROSITE; PS00432; ACTINS_2; 1. DR PROSITE; PS01132; ACTINS_ACT_LIKE; 1. PE 1: Evidence at protein level; KW Acetylation; ATP-binding; Cytoplasm; Cytoskeleton; Nucleotide-binding; KW Reference proteome. FT INIT_MET 1 FT /note="Removed" FT /evidence="ECO:0000250|UniProtKB:Q96292" FT CHAIN 2..377 FT /note="Actin-4" FT /id="PRO_0000088890" FT MOD_RES 2 FT /note="N-acetylalanine" FT /evidence="ECO:0000250|UniProtKB:Q96292" FT CONFLICT 154 FT /note="V -> F (in Ref. 4; AAM65657)" FT /evidence="ECO:0000305" SQ SEQUENCE 377 AA; 41779 MW; 3BC5B633D2BB088A CRC64; MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP EEHPVLLTEA PLNPKANREK MTQIMFETFN TPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDH LMKILTERGY SFTTTAEREI VRDMKEKLSY IALDFEQELE TSKTSSSVEK SFELPDGQVI TIGAERFRCP EVLFQPSMIG MENPGIHETT YNSIMKCDVD IRKDLYGNIV LSGGTTMFGG IGDRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIA KAEYDESGPS IVHRKCF //