ID MDS3_YEAST Reviewed; 1487 AA. AC P53094; D6VTV6; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 21-SEP-2011, sequence version 2. DT 21-SEP-2011, entry version 97. DE RecName: Full=Negative regulator of sporulation MDS3; DE AltName: Full=MCK1 dosage suppressor 3; GN Name=MDS3; OrderedLocusNames=YGL197W; ORFNames=G1307; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX MEDLINE=97197971; PubMed=9046087; RX DOI=10.1002/(SICI)1097-0061(199701)13:1<55::AID-YEA48>3.3.CO;2-0; RA Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., RA Bruschi C.V.; RT "Sequencing of a 40.5 kb fragment located on the left arm of RT chromosome VII from Saccharomyces cerevisiae."; RL Yeast 13:55-64(1997). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX MEDLINE=97313265; PubMed=9169869; RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., RA Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., RA Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., RA Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., RA Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., RA Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., RA Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., RA Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., RA Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., RA Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., RA Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., RA Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., RA Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., RA Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., RA Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., RA Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., RA Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., RA Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., RA Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., RA van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., RA Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., RA Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., RA Zollner A., Kleine K.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."; RL Nature 387:81-84(1997). RN [3] RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 262-264 AND 403. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases. RN [4] RP CHARACTERIZATION. RX PubMed=9383076; RA Benni M.L., Neigeborn L.; RT "Identification of a new class of negative regulators affecting RT sporulation-specific gene expression in yeast."; RL Genetics 147:1351-1366(1997). RN [5] RP FUNCTION. RX PubMed=12524333; RA Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.; RT "Candida albicans Mds3p, a conserved regulator of pH responses and RT virulence identified through insertional mutagenesis."; RL Genetics 162:1573-1581(2002). RN [6] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [7] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474 AND SER-639, AND RP MASS SPECTROMETRY. RC STRAIN=YAL6B; RX PubMed=15665377; DOI=10.1074/mcp.M400219-MCP200; RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., RA Mann M., Jensen O.N.; RT "Quantitative phosphoproteomics applied to the yeast pheromone RT signaling pathway."; RL Mol. Cell. Proteomics 4:310-327(2005). RN [9] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND RP SER-781, AND MASS SPECTROMETRY. RC STRAIN=ADR376; RX PubMed=17330950; DOI=10.1021/pr060559j; RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., RA Elias J.E., Gygi S.P.; RT "Large-scale phosphorylation analysis of alpha-factor-arrested RT Saccharomyces cerevisiae."; RL J. Proteome Res. 6:1190-1197(2007). RN [10] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-668; SER-693; SER-770; RP SER-919 AND THR-920, AND MASS SPECTROMETRY. RX PubMed=17563356; DOI=10.1073/pnas.0701622104; RA Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; RT "Proteome-wide identification of in vivo targets of DNA damage RT checkpoint kinases."; RL Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). RN [11] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-474; SER-618; SER-621; RP THR-636; SER-637; SER-667; SER-693; SER-698; SER-747; SER-757; RP SER-763; SER-769; SER-781; SER-828; THR-854; SER-904; SER-918; RP SER-919; THR-920; SER-934; THR-1156 AND SER-1162, AND MASS RP SPECTROMETRY. RX PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200; RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; RT "A multidimensional chromatography technology for in-depth RT phosphoproteome analysis."; RL Mol. Cell. Proteomics 7:1389-1396(2008). RN [12] RP FUNCTION. RX PubMed=19087957; DOI=10.1534/genetics.108.098434; RA McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., RA Winter E.; RT "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK RT Smk1 and spore morphogenesis in Saccharomyces cerevisiae."; RL Genetics 181:511-523(2009). CC -!- FUNCTION: Negatively regulates early sporulation-specific genes. CC Seems to exert its function by positively regulating the Ras/cAMP CC pathway. Required for growth under alkaline conditions. Acts CC synergetically with PMD1. CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD CC medium. CC -!- SIMILARITY: Contains 3 Kelch repeats. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X91837; CAA62947.1; -; Genomic_DNA. DR EMBL; Z72719; CAA96909.1; -; Genomic_DNA. DR EMBL; BK006941; DAA07917.2; -; Genomic_DNA. DR PIR; S62048; S62048. DR RefSeq; NP_011318.2; NM_001181062.2. DR ProteinModelPortal; P53094; -. DR SMR; P53094; 169-270. DR DIP; DIP-6381N; -. DR IntAct; P53094; 22. DR MINT; MINT-598198; -. DR STRING; P53094; -. DR EnsemblFungi; YGL197W; YGL197W; YGL197W. DR GeneID; 852678; -. DR KEGG; sce:YGL197W; -. DR NMPDR; fig|4932.3.peg.2421; -. DR CYGD; YGL197w; -. DR SGD; S000003165; MDS3. DR eggNOG; fuNOG04988; -. DR GeneTree; EFGT00050000001121; -. DR HOGENOM; HBG204455; -. DR OMA; GGMVFRV; -. DR OrthoDB; EOG437VP0; -. DR PhylomeDB; P53094; -. DR NextBio; 971987; -. DR ArrayExpress; P53094; -. DR Genevestigator; P53094; -. DR GermOnline; YGL197W; Saccharomyces cerevisiae. DR GO; GO:0005739; C:mitochondrion; IDA:SGD. DR GO; GO:0007126; P:meiosis; IEA:UniProtKB-KW. DR GO; GO:0042174; P:negative regulation of sporulation resulting in formation of a cellular spore; IMP:SGD. DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. DR GO; GO:0031929; P:TOR signaling cascade; IMP:SGD. DR InterPro; IPR015915; Kelch-typ_b-propeller. DR InterPro; IPR006652; Kelch_1. DR Gene3D; G3DSA:2.120.10.80; Kelch-typ_b-propeller; 3. DR Pfam; PF01344; Kelch_1; 1. PE 1: Evidence at protein level; KW Complete proteome; Cytoplasm; Kelch repeat; Meiosis; Phosphoprotein; KW Reference proteome; Repeat; Sporulation. FT CHAIN 1 1487 Negative regulator of sporulation MDS3. FT /FTId=PRO_0000119137. FT REPEAT 171 226 Kelch 1. FT REPEAT 234 287 Kelch 2. FT REPEAT 371 419 Kelch 3. FT MOD_RES 474 474 Phosphoserine. FT MOD_RES 618 618 Phosphoserine. FT MOD_RES 621 621 Phosphoserine. FT MOD_RES 636 636 Phosphothreonine. FT MOD_RES 637 637 Phosphoserine. FT MOD_RES 639 639 Phosphoserine. FT MOD_RES 667 667 Phosphoserine. FT MOD_RES 668 668 Phosphoserine. FT MOD_RES 693 693 Phosphoserine. FT MOD_RES 698 698 Phosphoserine. FT MOD_RES 747 747 Phosphoserine. FT MOD_RES 757 757 Phosphoserine. FT MOD_RES 763 763 Phosphoserine. FT MOD_RES 769 769 Phosphoserine. FT MOD_RES 770 770 Phosphoserine. FT MOD_RES 781 781 Phosphoserine. FT MOD_RES 828 828 Phosphoserine. FT MOD_RES 854 854 Phosphothreonine. FT MOD_RES 904 904 Phosphoserine. FT MOD_RES 918 918 Phosphoserine. FT MOD_RES 919 919 Phosphoserine. FT MOD_RES 920 920 Phosphothreonine. FT MOD_RES 934 934 Phosphoserine. FT MOD_RES 1156 1156 Phosphothreonine. FT MOD_RES 1162 1162 Phosphoserine. FT CONFLICT 262 264 QSE -> HPK (in Ref. 1; CAA62947 and 2; FT CAA96909). FT CONFLICT 403 403 A -> R (in Ref. 1; CAA62947 and 2; FT CAA96909). SQ SEQUENCE 1487 AA; 166971 MW; 31073D68ACA4AF61 CRC64; MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR SNIFVHGGLT IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK ETFFLDLMSR TWYRVKTSFD QRTEELLKAE SSSAKADNDT NEIRTDIKKG KSLESPLKER LFHSLCYLDG CLYIFGGLTV SPQSGYELIA TNELWKLDLN TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG LNNMDQPVKK IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL PKKLLNVPYE LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS RVATACPDCD INKHRFWRVF VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS TFGLPLVNIF NKTIQLPHHK ISASSLPIPI ENFAKHKDTP LKKVSFTSSA TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG KPLSDFEFIT SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN ADAKAPNTPS TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS NSIYRPIRKT ESSSTTSSSN GMIFRVPFKE KAAVTSNTEA LLESNLSLQE LSRRRSSLMS IPSGELLRSS ISEAEHQRRA SHPLTSSPLF EDSGTPCGKQ LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG NSISSRSTSD SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS QYTQPRMNSF PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL EPLLTPRSLY MPWPTSTVRA FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY EIPLLYKLIL EVLYSILAKK EESLSLICTS LMETFRTKTL NSYKGDEEKT NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ SSSTQESSGS ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF NLHLFDMNYG SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD ARFRNKEDDK VTKDISNDKK RNYLPHEKNN LKAKEGKETR DVREEEEEFD FGLGMLSLNK IKREAKHVDK VDDSVDPLFK SSAFPQSPIR AYGSTTRTSS ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY RTTVLVNDIR LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP //