ID MDS3_YEAST Reviewed; 1487 AA. AC P53094; D6VTV6; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 21-SEP-2011, sequence version 2. DT 07-APR-2021, entry version 165. DE RecName: Full=Negative regulator of sporulation MDS3; DE AltName: Full=MCK1 dosage suppressor 3; GN Name=MDS3; OrderedLocusNames=YGL197W; ORFNames=G1307; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX PubMed=9046087; RX DOI=10.1002/(sici)1097-0061(199701)13:1<55::aid-yea48>3.0.co;2-9; RA Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., Bruschi C.V.; RT "Sequencing of a 40.5 kb fragment located on the left arm of chromosome VII RT from Saccharomyces cerevisiae."; RL Yeast 13:55-64(1997). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=9169869; RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H., RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."; RL Nature 387:81-84(1997). RN [3] RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 262-264 AND 403. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). RN [4] RP CHARACTERIZATION. RX PubMed=9383076; RA Benni M.L., Neigeborn L.; RT "Identification of a new class of negative regulators affecting RT sporulation-specific gene expression in yeast."; RL Genetics 147:1351-1366(1997). RN [5] RP FUNCTION. RX PubMed=12524333; RA Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.; RT "Candida albicans Mds3p, a conserved regulator of pH responses and RT virulence identified through insertional mutagenesis."; RL Genetics 162:1573-1581(2002). RN [6] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [7] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., RA O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, AND IDENTIFICATION BY RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC STRAIN=YAL6B; RX PubMed=15665377; DOI=10.1074/mcp.m400219-mcp200; RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., RA Jensen O.N.; RT "Quantitative phosphoproteomics applied to the yeast pheromone signaling RT pathway."; RL Mol. Cell. Proteomics 4:310-327(2005). RN [9] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND SER-781, AND RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC STRAIN=ADR376; RX PubMed=17330950; DOI=10.1021/pr060559j; RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., RA Elias J.E., Gygi S.P.; RT "Large-scale phosphorylation analysis of alpha-factor-arrested RT Saccharomyces cerevisiae."; RL J. Proteome Res. 6:1190-1197(2007). RN [10] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693; SER-698 AND RP SER-904, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200; RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; RT "A multidimensional chromatography technology for in-depth phosphoproteome RT analysis."; RL Mol. Cell. Proteomics 7:1389-1396(2008). RN [11] RP FUNCTION. RX PubMed=19087957; DOI=10.1534/genetics.108.098434; RA McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., Winter E.; RT "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK Smk1 RT and spore morphogenesis in Saccharomyces cerevisiae."; RL Genetics 181:511-523(2009). RN [12] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472; SER-475; SER-621; RP SER-693; SER-744; SER-747; SER-756; SER-757; SER-781; THR-785; SER-787; RP SER-900; THR-930 AND SER-1187, AND IDENTIFICATION BY MASS SPECTROMETRY RP [LARGE SCALE ANALYSIS]. RX PubMed=19779198; DOI=10.1126/science.1172867; RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.; RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights RT into evolution."; RL Science 325:1682-1686(2009). CC -!- FUNCTION: Negatively regulates early sporulation-specific genes. Seems CC to exert its function by positively regulating the Ras/cAMP pathway. CC Required for growth under alkaline conditions. Acts synergetically with CC PMD1. {ECO:0000269|PubMed:12524333, ECO:0000269|PubMed:19087957}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. CC -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium. CC {ECO:0000269|PubMed:14562106}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X91837; CAA62947.1; -; Genomic_DNA. DR EMBL; Z72719; CAA96909.1; -; Genomic_DNA. DR EMBL; BK006941; DAA07917.2; -; Genomic_DNA. DR PIR; S62048; S62048. DR RefSeq; NP_011318.2; NM_001181062.2. DR BioGRID; 33060; 154. DR DIP; DIP-6381N; -. DR IntAct; P53094; 25. DR MINT; P53094; -. DR STRING; 4932.YGL197W; -. DR iPTMnet; P53094; -. DR MaxQB; P53094; -. DR PaxDb; P53094; -. DR PRIDE; P53094; -. DR EnsemblFungi; YGL197W_mRNA; YGL197W; YGL197W. DR GeneID; 852678; -. DR KEGG; sce:YGL197W; -. DR SGD; S000003165; MDS3. DR VEuPathDB; FungiDB:YGL197W; -. DR eggNOG; ENOG502QSQ9; Eukaryota. DR GeneTree; ENSGT00940000176594; -. DR HOGENOM; CLU_252311_0_0_1; -. DR InParanoid; P53094; -. DR OMA; WQSHHKV; -. DR Reactome; R-SCE-6798695; Neutrophil degranulation. DR PRO; PR:P53094; -. DR Proteomes; UP000002311; Chromosome VII. DR RNAct; P53094; protein. DR GO; GO:0005737; C:cytoplasm; HDA:SGD. DR GO; GO:0005829; C:cytosol; IBA:GO_Central. DR GO; GO:0005739; C:mitochondrion; HDA:SGD. DR GO; GO:0045454; P:cell redox homeostasis; IBA:GO_Central. DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW. DR GO; GO:0075297; P:negative regulation of ascospore formation; IMP:SGD. DR GO; GO:0007124; P:pseudohyphal growth; IMP:SGD. DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. DR GO; GO:0031929; P:TOR signaling; IMP:SGD. DR Gene3D; 2.120.10.80; -; 1. DR InterPro; IPR015915; Kelch-typ_b-propeller. DR SUPFAM; SSF117281; SSF117281; 1. PE 1: Evidence at protein level; KW Cytoplasm; Kelch repeat; Meiosis; Phosphoprotein; Reference proteome; KW Repeat; Sporulation. FT CHAIN 1..1487 FT /note="Negative regulator of sporulation MDS3" FT /id="PRO_0000119137" FT REPEAT 171..226 FT /note="Kelch 1" FT REPEAT 234..287 FT /note="Kelch 2" FT REPEAT 371..419 FT /note="Kelch 3" FT MOD_RES 472 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 475 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 621 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:17330950, FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198" FT MOD_RES 639 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:15665377" FT MOD_RES 693 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:17330950, FT ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198" FT MOD_RES 698 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:18407956" FT MOD_RES 744 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 747 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 756 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 757 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 781 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:17330950, FT ECO:0007744|PubMed:19779198" FT MOD_RES 785 FT /note="Phosphothreonine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 787 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 900 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 904 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:18407956" FT MOD_RES 930 FT /note="Phosphothreonine" FT /evidence="ECO:0007744|PubMed:19779198" FT MOD_RES 1187 FT /note="Phosphoserine" FT /evidence="ECO:0007744|PubMed:19779198" FT CONFLICT 262..264 FT /note="QSE -> HPK (in Ref. 1; CAA62947 and 2; CAA96909)" FT /evidence="ECO:0000305" FT CONFLICT 403 FT /note="A -> R (in Ref. 1; CAA62947 and 2; CAA96909)" FT /evidence="ECO:0000305" SQ SEQUENCE 1487 AA; 166971 MW; 31073D68ACA4AF61 CRC64; MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR SNIFVHGGLT IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK ETFFLDLMSR TWYRVKTSFD QRTEELLKAE SSSAKADNDT NEIRTDIKKG KSLESPLKER LFHSLCYLDG CLYIFGGLTV SPQSGYELIA TNELWKLDLN TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG LNNMDQPVKK IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL PKKLLNVPYE LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS RVATACPDCD INKHRFWRVF VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS TFGLPLVNIF NKTIQLPHHK ISASSLPIPI ENFAKHKDTP LKKVSFTSSA TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG KPLSDFEFIT SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN ADAKAPNTPS TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS NSIYRPIRKT ESSSTTSSSN GMIFRVPFKE KAAVTSNTEA LLESNLSLQE LSRRRSSLMS IPSGELLRSS ISEAEHQRRA SHPLTSSPLF EDSGTPCGKQ LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG NSISSRSTSD SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS QYTQPRMNSF PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL EPLLTPRSLY MPWPTSTVRA FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY EIPLLYKLIL EVLYSILAKK EESLSLICTS LMETFRTKTL NSYKGDEEKT NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ SSSTQESSGS ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF NLHLFDMNYG SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD ARFRNKEDDK VTKDISNDKK RNYLPHEKNN LKAKEGKETR DVREEEEEFD FGLGMLSLNK IKREAKHVDK VDDSVDPLFK SSAFPQSPIR AYGSTTRTSS ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY RTTVLVNDIR LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP //