ID MDS3_YEAST Reviewed; 1487 AA. AC P53094; D6VTV6; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 21-SEP-2011, sequence version 2. DT 09-DEC-2015, entry version 131. DE RecName: Full=Negative regulator of sporulation MDS3; DE AltName: Full=MCK1 dosage suppressor 3; GN Name=MDS3; OrderedLocusNames=YGL197W; ORFNames=G1307; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX PubMed=9046087; RX DOI=10.1002/(SICI)1097-0061(199701)13:1<55::AID-YEA48>3.3.CO;2-0; RA Coglievina M., Klima R., Bertani I., Delneri D., Zaccaria P., RA Bruschi C.V.; RT "Sequencing of a 40.5 kb fragment located on the left arm of RT chromosome VII from Saccharomyces cerevisiae."; RL Yeast 13:55-64(1997). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=9169869; RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., RA Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., RA Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., RA Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., RA Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., RA Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., RA Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., RA Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., RA Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., RA Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., RA Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., RA Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., RA Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., RA Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., RA Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., RA Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., RA Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., RA Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., RA Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., RA van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., RA Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., RA Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., RA Zollner A., Kleine K.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."; RL Nature 387:81-84(1997). RN [3] RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 262-264 AND 403. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., RA Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and RT now."; RL G3 (Bethesda) 4:389-398(2014). RN [4] RP CHARACTERIZATION. RX PubMed=9383076; RA Benni M.L., Neigeborn L.; RT "Identification of a new class of negative regulators affecting RT sporulation-specific gene expression in yeast."; RL Genetics 147:1351-1366(1997). RN [5] RP FUNCTION. RX PubMed=12524333; RA Davis D.A., Bruno V.M., Loza L., Filler S.G., Mitchell A.P.; RT "Candida albicans Mds3p, a conserved regulator of pH responses and RT virulence identified through insertional mutagenesis."; RL Genetics 162:1573-1581(2002). RN [6] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [7] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-639, AND IDENTIFICATION RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC STRAIN=YAL6B; RX PubMed=15665377; DOI=10.1074/mcp.M400219-MCP200; RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., RA Mann M., Jensen O.N.; RT "Quantitative phosphoproteomics applied to the yeast pheromone RT signaling pathway."; RL Mol. Cell. Proteomics 4:310-327(2005). RN [9] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693 AND RP SER-781, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE RP ANALYSIS]. RC STRAIN=ADR376; RX PubMed=17330950; DOI=10.1021/pr060559j; RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., RA Elias J.E., Gygi S.P.; RT "Large-scale phosphorylation analysis of alpha-factor-arrested RT Saccharomyces cerevisiae."; RL J. Proteome Res. 6:1190-1197(2007). RN [10] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621; SER-693; SER-698 RP AND SER-904, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE RP ANALYSIS]. RX PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200; RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; RT "A multidimensional chromatography technology for in-depth RT phosphoproteome analysis."; RL Mol. Cell. Proteomics 7:1389-1396(2008). RN [11] RP FUNCTION. RX PubMed=19087957; DOI=10.1534/genetics.108.098434; RA McDonald C.M., Wagner M., Dunham M.J., Shin M.E., Ahmed N.T., RA Winter E.; RT "The Ras/cAMP pathway and the CDK-like kinase Ime2 regulate the MAPK RT Smk1 and spore morphogenesis in Saccharomyces cerevisiae."; RL Genetics 181:511-523(2009). RN [12] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-472; SER-475; SER-621; RP SER-693; SER-744; SER-747; SER-756; SER-757; SER-781; THR-785; RP SER-787; SER-900; THR-930 AND SER-1187, AND IDENTIFICATION BY MASS RP SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=19779198; DOI=10.1126/science.1172867; RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.; RT "Global analysis of Cdk1 substrate phosphorylation sites provides RT insights into evolution."; RL Science 325:1682-1686(2009). CC -!- FUNCTION: Negatively regulates early sporulation-specific genes. CC Seems to exert its function by positively regulating the Ras/cAMP CC pathway. Required for growth under alkaline conditions. Acts CC synergetically with PMD1. {ECO:0000269|PubMed:12524333, CC ECO:0000269|PubMed:19087957}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. CC -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD CC medium. {ECO:0000269|PubMed:14562106}. CC -!- SIMILARITY: Contains 3 Kelch repeats. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X91837; CAA62947.1; -; Genomic_DNA. DR EMBL; Z72719; CAA96909.1; -; Genomic_DNA. DR EMBL; BK006941; DAA07917.2; -; Genomic_DNA. DR PIR; S62048; S62048. DR RefSeq; NP_011318.2; NM_001181062.2. DR BioGrid; 33060; 78. DR DIP; DIP-6381N; -. DR IntAct; P53094; 24. DR MINT; MINT-598198; -. DR MaxQB; P53094; -. DR EnsemblFungi; YGL197W; YGL197W; YGL197W. DR GeneID; 852678; -. DR KEGG; sce:YGL197W; -. DR EuPathDB; FungiDB:YGL197W; -. DR SGD; S000003165; MDS3. DR GeneTree; ENSGT00530000066388; -. DR HOGENOM; HOG000113513; -. DR InParanoid; P53094; -. DR OMA; HDYGSHR; -. DR OrthoDB; EOG7HQNHW; -. DR BioCyc; YEAST:G3O-30678-MONOMER; -. DR NextBio; 971987; -. DR PRO; PR:P53094; -. DR Proteomes; UP000002311; Chromosome VII. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW. DR GO; GO:0042174; P:negative regulation of sporulation resulting in formation of a cellular spore; IMP:SGD. DR GO; GO:0007124; P:pseudohyphal growth; IMP:SGD. DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. DR GO; GO:0031929; P:TOR signaling; IMP:SGD. DR Gene3D; 2.120.10.80; -; 3. DR InterPro; IPR015915; Kelch-typ_b-propeller. PE 1: Evidence at protein level; KW Complete proteome; Cytoplasm; Kelch repeat; Meiosis; Phosphoprotein; KW Reference proteome; Repeat; Sporulation. FT CHAIN 1 1487 Negative regulator of sporulation MDS3. FT /FTId=PRO_0000119137. FT REPEAT 171 226 Kelch 1. FT REPEAT 234 287 Kelch 2. FT REPEAT 371 419 Kelch 3. FT MOD_RES 472 472 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 475 475 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 621 621 Phosphoserine. FT {ECO:0000244|PubMed:17330950, FT ECO:0000244|PubMed:18407956, FT ECO:0000244|PubMed:19779198}. FT MOD_RES 639 639 Phosphoserine. FT {ECO:0000244|PubMed:15665377}. FT MOD_RES 693 693 Phosphoserine. FT {ECO:0000244|PubMed:17330950, FT ECO:0000244|PubMed:18407956, FT ECO:0000244|PubMed:19779198}. FT MOD_RES 698 698 Phosphoserine. FT {ECO:0000244|PubMed:18407956}. FT MOD_RES 744 744 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 747 747 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 756 756 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 757 757 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 781 781 Phosphoserine. FT {ECO:0000244|PubMed:17330950, FT ECO:0000244|PubMed:19779198}. FT MOD_RES 785 785 Phosphothreonine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 787 787 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 900 900 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 904 904 Phosphoserine. FT {ECO:0000244|PubMed:18407956}. FT MOD_RES 930 930 Phosphothreonine. FT {ECO:0000244|PubMed:19779198}. FT MOD_RES 1187 1187 Phosphoserine. FT {ECO:0000244|PubMed:19779198}. FT CONFLICT 262 264 QSE -> HPK (in Ref. 1; CAA62947 and 2; FT CAA96909). {ECO:0000305}. FT CONFLICT 403 403 A -> R (in Ref. 1; CAA62947 and 2; FT CAA96909). {ECO:0000305}. SQ SEQUENCE 1487 AA; 166971 MW; 31073D68ACA4AF61 CRC64; MPLLQPSTCF CYPLKLPPLP LTSDSNEFDE CARKRLTLDY RTGSAVTLTR SNIFVHGGLT IPLNLPVVNS MQLQKELILF FAKEKNNGSS FRNLNEWISK ETFFLDLMSR TWYRVKTSFD QRTEELLKAE SSSAKADNDT NEIRTDIKKG KSLESPLKER LFHSLCYLDG CLYIFGGLTV SPQSGYELIA TNELWKLDLN TKKWSLLSDD PQIARRFNHT MHVKNENNDN RDTKLIIVGG LNNMDQPVKK IDIYNISQNC WQSETIPKQP MEITTNVNGI PLALSKDQNF SILVENNEAN VPALAFYMRS DQIDEYLGKD SSKIKENSPI VALPLLSESQ GIRMPSNPAL PKKLLNVPYE LLAPTGDYFG FNIIIGGFHP NYQSSNFHCF IYDINSGKWS RVATACPDCD INKHRFWRVF VWKSHHQTIL LGTKTDDYYS PSVQRFDHLS TFGLPLVNIF NKTIQLPHHK ISASSLPIPI ENFAKHKDTP LKKVSFTSSA TSQFENYIRY IAPPLEMSSI QSVFPPYAMV LGKDALEIYG KPLSDFEFIT SEGDSIGIPC YLLRKRWGRY FDMLLSQSYT KVCADYETTD TQSTLIKFSP HSSRNSSKAV RQEGRLSSSG SLDNYFEKNF PIFARTSVSE AQNTQPQVAN ADAKAPNTPS TSDEPSSSSS SDLYSTPHYQ RNNDEEDDED PVSPKPVSKS NSIYRPIRKT ESSSTTSSSN GMIFRVPFKE KAAVTSNTEA LLESNLSLQE LSRRRSSLMS IPSGELLRSS ISEAEHQRRA SHPLTSSPLF EDSGTPCGKQ LQQLQQHTIQ NPHNHLSPRR FSRSARSSIS YVSSSSDRRG NSISSRSTSD SFGTPPVLGV LNVPLPPQTR EPNEPPPPCP AMSTGSNTRR SNTLTDYMHS NKASPFSSRR SSHIGRRSST PETENAFSAT PRASLDGQML GKSLKEGSTS QYTQPRMNSF PKANETIQTP TSSNNEWSRQ SVTSNTDSFD SLQSNFALEL EPLLTPRSLY MPWPTSTVRA FAEFFYTGQV NSKWLLAPVA LDLLVMAKIY EIPLLYKLIL EVLYSILAKK EESLSLICTS LMETFRTKTL NSYKGDEEKT NTYLTSNDNY QELLKLKVSL ENIDNGYYDP DLLRKQSRAQ SSSTQESSGS ANGEKTATGA GSLETSSTNV PTVFAGGPRD SHNSVGSIGF PNSMNIQGSR RSTSGFSPRV KMKSSLSKEI DPKTFYEEYE PKEGKSFDDN DDQQTNIGSF NLHLFDMNYG SISSSSTNSI SSSDLEEKEE QEQLQDLLEI EREDSAEILD ARFRNKEDDK VTKDISNDKK RNYLPHEKNN LKAKEGKETR DVREEEEEFD FGLGMLSLNK IKREAKHVDK VDDSVDPLFK SSAFPQSPIR AYGSTTRTSS ASGKPFRDNR SFNAFSVLTL ENMASANALP PVDYVIKSIY RTTVLVNDIR LMVRCMDCIE LSKNLRALKK KTMEDISKLK GISKPSP //