ID UFD1_YEAST Reviewed; 361 AA. AC P53044; Q45U36; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 1. DT 29-APR-2008, entry version 63. DE Ubiquitin fusion degradation protein 1 (UB fusion protein 1) DE (Polymerase-interacting protein 3). GN Name=UFD1; Synonyms=PIP3; OrderedLocusNames=YGR048W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF VAL-94. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=95340540; PubMed=7615550; DOI=10.1074/jbc.270.29.17442; RA Johnson E.S., Ma P.C.M., Ota I.M., Varshavsky A.; RT "A proteolytic pathway that recognizes ubiquitin as a degradation RT signal."; RL J. Biol. Chem. 270:17442-17456(1995). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 200060 / W303; RX MEDLINE=97379980; PubMed=9236779; DOI=10.1007/s004380050491; RA del Olmo M., Mizrahi N., Gross S., Moore C.L.; RT "The Uba2 and Ufd1 proteins of Saccharomyces cerevisiae interact with RT poly(A) polymerase and affect the polyadenylation activity of cell RT extracts."; RL Mol. Gen. Genet. 255:209-218(1997). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=SK1; RX PubMed=16273108; DOI=10.1038/ng1674; RA Deutschbauer A.M., Davis R.W.; RT "Quantitative trait loci mapped to single-nucleotide resolution in RT yeast."; RL Nat. Genet. 37:1333-1340(2005). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX MEDLINE=97313265; PubMed=9169869; RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., RA Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., RA Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., RA Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., RA Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., RA Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., RA Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., RA Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., RA Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., RA Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., RA Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., RA Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., RA Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., RA Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., RA Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., RA Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., RA Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., RA Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., RA Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., RA van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., RA Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., RA Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., RA Zollner A., Kleine K.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."; RL Nature 387:81-84(1997). RN [5] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=17322287; DOI=10.1101/gr.6037607; RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., RA Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., RA Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., RA Kolodner R.D., LaBaer J.; RT "Approaching a complete repository of sequence-verified protein- RT encoding clones for Saccharomyces cerevisiae."; RL Genome Res. 17:536-543(2007). RN [6] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, AND MASS RP SPECTROMETRY. RX PubMed=15665377; DOI=10.1074/mcp.M400219-MCP200; RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., RA Mann M., Jensen O.N.; RT "Quantitative phosphoproteomics applied to the yeast pheromone RT signaling pathway."; RL Mol. Cell. Proteomics 4:310-327(2005). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, AND MASS RP SPECTROMETRY. RX PubMed=17330950; DOI=10.1021/pr060559j; RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., RA Elias J.E., Gygi S.P.; RT "Large-scale phosphorylation analysis of alpha-factor-arrested RT Saccharomyces cerevisiae."; RL J. Proteome Res. 6:1190-1197(2007). RN [9] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, AND MASS RP SPECTROMETRY. RX PubMed=17563356; DOI=10.1073/pnas.0701622104; RA Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; RT "Proteome-wide identification of in vivo targets of DNA damage RT checkpoint kinases."; RL Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). CC -!- FUNCTION: Functions at a post-ubiquitation step in the ubiquitin CC fusion degradation (UFD) pathway. It is required for vegetative CC growth. CC -!- INTERACTION: CC P33755:NPL4; NbExp=1; IntAct=EBI-19997, EBI-12193; CC -!- MISCELLANEOUS: Present with 3530 molecules/cell in log phase SD CC medium. CC -!- SIMILARITY: Belongs to the UFD1 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U22153; AAC49023.1; -; Genomic_DNA. DR EMBL; U17264; AAB46627.1; -; Genomic_DNA. DR EMBL; DQ115391; AAZ22463.1; -; Genomic_DNA. DR EMBL; Z72833; CAA97047.1; -; Genomic_DNA. DR EMBL; AY692806; AAT92825.1; -; Genomic_DNA. DR PIR; S59814; S59814. DR RefSeq; NP_011562.1; -. DR PDB; 1ZC1; NMR; -; A=1-208. DR PDBsum; 1ZC1; -. DR DIP; DIP:1476N; -. DR IntAct; P53044; -. DR PeptideAtlas; P53044; -. DR Ensembl; YGR048W; Saccharomyces cerevisiae. DR GeneID; 852939; -. DR GenomeReviews; Y13135_GR; YGR048W. DR KEGG; sce:YGR048W; -. DR NMPDR; fig|4932.3.peg.2678; -. DR CYGD; YGR048w; -. DR SGD; S000003280; UFD1. DR LinkHub; P53044; -. DR ArrayExpress; P53044; -. DR GermOnline; YGR048W; Saccharomyces cerevisiae. DR GO; GO:0034098; C:Cdc48p-Npl4p-Ufd1p AAA ATPase complex; IDA:SGD. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0015031; P:protein transport; IDA:SGD. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:SGD. DR InterPro; IPR004854; UFD1. DR PANTHER; PTHR12555; UFD1; 1. DR Pfam; PF03152; UFD1; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Phosphoprotein; KW Ubl conjugation pathway. FT CHAIN 1 361 Ubiquitin fusion degradation protein 1. FT /FTId=PRO_0000194989. FT MOD_RES 354 354 Phosphoserine. FT MUTAGEN 94 94 V->D: In UFD1-1; grows more slowly. FT STRAND 19 29 FT HELIX 30 32 FT TURN 35 37 FT HELIX 40 44 FT STRAND 45 50 FT HELIX 52 60 FT STRAND 69 72 FT TURN 74 76 FT STRAND 79 87 FT STRAND 93 96 FT HELIX 98 104 FT STRAND 111 118 FT STRAND 123 128 FT HELIX 131 135 FT HELIX 140 150 FT STRAND 154 164 FT STRAND 167 177 FT STRAND 188 190 FT STRAND 192 196 SQ SEQUENCE 361 AA; 39810 MW; 198E0CD48B863B98 CRC64; MFSGFSSFGG GNGFVNMPQT FEEFFRCYPI AMMNDRIRKD DANFGGKIFL PPSALSKLSM LNIRYPMLFK LTANETGRVT HGGVLEFIAE EGRVYLPQWM METLGIQPGS LLQISSTDVP LGQFVKLEPQ SVDFLDISDP KAVLENVLRN FSTLTVDDVI EISYNGKTFK IKILEVKPES SSKSICVIET DLVTDFAPPV GYVEPDYKAL KAQQDKEKKN SFGKGQVLDP SVLGQGSMST RIDYAGIANS SRNKLSKFVG QGQNISGKAP KAEPKQDIKD MKITFDGEPA KLDLPEGQLF FGFPMVLPKE DEESAAGSKS SEQNFQGQGI SLRKSNKRKT KSDHDSSKSK APKSPEVIEI D //