ID UFD1_YEAST Reviewed; 361 AA. AC P53044; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 1. DT 06-FEB-2007, entry version 49. DE Ubiquitin fusion degradation protein 1 (UB fusion protein 1) DE (Polymerase-interacting protein 3). GN Name=UFD1; Synonyms=PIP3; OrderedLocusNames=YGR048W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF VAL-94. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=95340540; PubMed=7615550; DOI=10.1074/jbc.270.29.17442; RA Johnson E.S., Ma P.C.M., Ota I.M., Varshavsky A.; RT "A proteolytic pathway that recognizes ubiquitin as a degradation RT signal."; RL J. Biol. Chem. 270:17442-17456(1995). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 200060 / W303; RX MEDLINE=97379980; PubMed=9236779; DOI=10.1007/s004380050491; RA del Olmo M., Mizrahi N., Gross S., Moore C.L.; RT "The Uba2 and Ufd1 proteins of Saccharomyces cerevisiae interact with RT poly(A) polymerase and affect the polyadenylation activity of cell RT extracts."; RL Mol. Gen. Genet. 255:209-218(1997). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX MEDLINE=97313265; PubMed=9169869; RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., RA Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., RA Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., RA Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., RA Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., RA Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., RA Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., RA Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., RA Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., RA Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., RA Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., RA Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., RA Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., RA Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., RA Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., RA Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., RA Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., RA Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., RA Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., RA van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., RA Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., RA Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., RA Zollner A., Kleine K.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."; RL Nature 387:81-84(1997). RN [4] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., RA LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces RT cerevisiae ORFs in the Gateway recombinational cloning system."; RL Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases. RN [5] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [6] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, AND MASS RP SPECTROMETRY. RX PubMed=15665377; DOI=10.1074/mcp.M400219-MCP200; RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., RA Mann M., Jensen O.N.; RT "Quantitative phosphoproteomics applied to the yeast pheromone RT signaling pathway."; RL Mol. Cell. Proteomics 4:310-327(2005). CC -!- FUNCTION: Functions at a post-ubiquitation step in the ubiquitin CC fusion degradation (UFD) pathway. It is required for vegetative CC growth. CC -!- INTERACTION: CC P33755:NPL4; NbExp=1; IntAct=EBI-19997, EBI-12193; CC -!- MISCELLANEOUS: Present with 3530 molecules/cell. CC -!- SIMILARITY: Belongs to the UFD1 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U22153; AAC49023.1; -; Genomic_DNA. DR EMBL; U17264; AAB46627.1; -; Genomic_DNA. DR EMBL; Z72833; CAA97047.1; -; Genomic_DNA. DR EMBL; AY692806; AAT92825.1; -; Genomic_DNA. DR PIR; S59814; S59814. DR PDB; 1ZC1; NMR; A=1-208. DR DIP; DIP:1476N; -. DR IntAct; P53044; -. DR Ensembl; YGR048W; Saccharomyces cerevisiae. DR GenomeReviews; Y13135_GR; YGR048W. DR KEGG; sce:YGR048W; -. DR SGD; S000003280; UFD1. DR BioCyc; SCER-S28-01:SCER-S28-01-002317-MONOMER; -. DR LinkHub; P53044; -. DR GermOnline; YGR048W; Saccharomyces cerevisiae. DR GO; GO:0005783; C:endoplasmic reticulum; IDA:SGD. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0006397; P:mRNA processing; TAS:SGD. DR GO; GO:0015031; P:protein transport; IDA:SGD. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; TAS:SGD. DR InterPro; IPR004854; UFD1. DR PANTHER; PTHR12555; UFD1; 1. DR Pfam; PF03152; UFD1; 1. KW 3D-structure; Complete proteome; Phosphorylation; KW Ubl conjugation pathway. FT CHAIN 1 361 Ubiquitin fusion degradation protein 1. FT /FTId=PRO_0000194989. FT MOD_RES 354 354 Phosphoserine. FT MUTAGEN 94 94 V->D: In UFD1-1; grows more slowly. FT TURN 12 13 FT STRAND 19 29 FT HELIX 30 32 FT TURN 35 37 FT HELIX 40 44 FT STRAND 45 50 FT HELIX 52 60 FT TURN 61 62 FT STRAND 69 72 FT TURN 74 76 FT STRAND 79 87 FT STRAND 93 96 FT HELIX 98 104 FT TURN 105 105 FT TURN 108 109 FT STRAND 111 118 FT STRAND 123 128 FT HELIX 131 135 FT TURN 136 136 FT HELIX 140 150 FT STRAND 154 164 FT TURN 165 166 FT STRAND 167 177 FT TURN 181 182 FT STRAND 188 190 FT STRAND 192 196 SQ SEQUENCE 361 AA; 39810 MW; 198E0CD48B863B98 CRC64; MFSGFSSFGG GNGFVNMPQT FEEFFRCYPI AMMNDRIRKD DANFGGKIFL PPSALSKLSM LNIRYPMLFK LTANETGRVT HGGVLEFIAE EGRVYLPQWM METLGIQPGS LLQISSTDVP LGQFVKLEPQ SVDFLDISDP KAVLENVLRN FSTLTVDDVI EISYNGKTFK IKILEVKPES SSKSICVIET DLVTDFAPPV GYVEPDYKAL KAQQDKEKKN SFGKGQVLDP SVLGQGSMST RIDYAGIANS SRNKLSKFVG QGQNISGKAP KAEPKQDIKD MKITFDGEPA KLDLPEGQLF FGFPMVLPKE DEESAAGSKS SEQNFQGQGI SLRKSNKRKT KSDHDSSKSK APKSPEVIEI D //