ID IF_MOUSE Reviewed; 417 AA. AC P52787; Q8C5C1; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 03-MAR-2009, sequence version 2. DT 14-OCT-2015, entry version 108. DE RecName: Full=Gastric intrinsic factor; DE AltName: Full=Intrinsic factor; DE Short=IF; DE Short=INF; DE Flags: Precursor; GN Name=Gif; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. RC STRAIN=BALB/c; TISSUE=Stomach; RX PubMed=8253786; RA Lorenz R.G., Gordon J.I.; RT "Use of transgenic mice to study regulation of gene expression in the RT parietal cell lineage of gastric units."; RL J. Biol. Chem. 268:26559-26570(1993). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=C57BL/6J; TISSUE=Cecum; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., RA Davis M.J., Wilming L.G., Aidinis V., Allen J.E., RA Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., RA Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., RA Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., RA Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., RA di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., RA Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., RA Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., RA Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., RA Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., RA Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., RA Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., RA Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., RA Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., RA Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., RA Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., RA Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., RA Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., RA Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., RA Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., RA Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., RA Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., RA Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., RA Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., RA Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., RA Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., RA Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., RA Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., RA Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., RA Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., RA Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.; RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). CC -!- FUNCTION: Promotes absorption of the essential vitamin cobalamin CC (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin CC complex is internalized via receptor-mediated endocytosis (By CC similarity). {ECO:0000250}. CC -!- SUBUNIT: Interacts with CUBN (via CUB domains). {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- TISSUE SPECIFICITY: Gastric mucosa. CC -!- SIMILARITY: Belongs to the eukaryotic cobalamin transport proteins CC family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L24192; AAA37881.1; -; Genomic_DNA. DR EMBL; L24191; AAA37882.1; -; mRNA. DR EMBL; AK078933; BAC37468.1; -; mRNA. DR EMBL; CH466534; EDL41439.1; -; Genomic_DNA. DR EMBL; BC118519; AAI18520.1; -; mRNA. DR CCDS; CCDS29609.1; -. DR PIR; A49684; A49684. DR RefSeq; NP_032144.2; NM_008118.3. DR UniGene; Mm.456; -. DR ProteinModelPortal; P52787; -. DR SMR; P52787; 25-417. DR STRING; 10090.ENSMUSP00000025585; -. DR PhosphoSite; P52787; -. DR MaxQB; P52787; -. DR PRIDE; P52787; -. DR Ensembl; ENSMUST00000025585; ENSMUSP00000025585; ENSMUSG00000024682. DR GeneID; 14603; -. DR KEGG; mmu:14603; -. DR UCSC; uc008gsw.1; mouse. DR CTD; 2694; -. DR MGI; MGI:1202394; Gif. DR eggNOG; NOG254667; -. DR GeneTree; ENSGT00530000063370; -. DR HOGENOM; HOG000013214; -. DR HOVERGEN; HBG006133; -. DR InParanoid; P52787; -. DR KO; K14615; -. DR OMA; NLAGAYN; -. DR OrthoDB; EOG7H1JKX; -. DR PhylomeDB; P52787; -. DR TreeFam; TF333092; -. DR Reactome; R-MMU-196741; Cobalamin (Cbl, vitamin B12) transport and metabolism. DR NextBio; 286384; -. DR PRO; PR:P52787; -. DR Proteomes; UP000000589; Chromosome 19. DR Bgee; P52787; -. DR CleanEx; MM_GIF; -. DR Genevisible; P52787; MM. DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI. DR GO; GO:0005768; C:endosome; ISO:MGI. DR GO; GO:0005615; C:extracellular space; ISO:MGI. DR GO; GO:0005902; C:microvillus; ISO:MGI. DR GO; GO:0031419; F:cobalamin binding; ISO:MGI. DR GO; GO:0015889; P:cobalamin transport; ISO:MGI. DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW. DR InterPro; IPR002157; Cbl-bd_transpt_euk. DR InterPro; IPR027954; DUF4430. DR PANTHER; PTHR10559; PTHR10559; 1. DR Pfam; PF01122; Cobalamin_bind; 1. DR Pfam; PF14478; DUF4430; 1. DR PROSITE; PS00468; COBALAMIN_BINDING; 1. PE 2: Evidence at transcript level; KW Cobalt; Cobalt transport; Complete proteome; Disulfide bond; KW Glycoprotein; Ion transport; Phosphoprotein; Reference proteome; KW Secreted; Signal; Transport. FT SIGNAL 1 18 {ECO:0000255}. FT CHAIN 19 417 Gastric intrinsic factor. FT /FTId=PRO_0000005559. FT REGION 365 370 Cobalamin binding. {ECO:0000250}. FT REGION 386 395 Cobalamin binding. {ECO:0000250}. FT BINDING 171 171 Cobalamin. {ECO:0000250}. FT BINDING 222 222 Cobalamin. {ECO:0000250}. FT BINDING 270 270 Cobalamin. {ECO:0000250}. FT MOD_RES 191 191 Phosphoserine. FT {ECO:0000250|UniProtKB:P17267}. FT CARBOHYD 311 311 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 330 330 N-linked (GlcNAc...). {ECO:0000255}. FT CARBOHYD 413 413 N-linked (GlcNAc...). {ECO:0000255}. FT DISULFID 26 246 {ECO:0000250}. FT DISULFID 103 288 {ECO:0000250}. FT DISULFID 143 182 {ECO:0000250}. FT CONFLICT 86 86 D -> N (in Ref. 1; AAA37881/AAA37882). FT {ECO:0000305}. FT CONFLICT 156 156 R -> A (in Ref. 1; AAA37881/AAA37882). FT {ECO:0000305}. FT CONFLICT 327 327 L -> P (in Ref. 1; AAA37881/AAA37882). FT {ECO:0000305}. SQ SEQUENCE 417 AA; 45497 MW; BF38663EFC90F34A CRC64; MAWLTLYLLS VLWAVAGTST RAQSSCSVPP DQQPWVDGLQ ALMENSVTDS DFPNPSILIA MNLAGAYNVE AQKLLTYQLM ASDSADLTSG QLALTVMALT SSCRDPGSKV STLLKKMENW SPSSPGAESS AFYGPGLAIL ALCQKSSEAT LPIAVRFAKT LMMEPSPFNV DTGAVATLAL TCMYNKIPVG SQENYRDLFG QALKAIVEKI SLRIKADGII GDIYSTGLAM QALSVTPEQP TKKWDCEKTM HTILNEIKQG KFQNPMSIAQ ILPSLKGKTY LDVPQVTCGP DHEVPPTLTD YPTPVPTSVS NITVIYTINN QLRGVDLLFN VTIEVSVKSG SVLLAVLEEA QRKNSMFKFE TTMTSWGLIV SSINNIAENV NHKTYWEFLS GKTPLDEGVA YYIPFNHEHI TANFTQY //