ID   R144A_HUMAN             Reviewed;         292 AA.
AC   P50876; D6W4Y6; Q585H5;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 2.
DT   21-SEP-2011, entry version 100.
DE   RecName: Full=Probable E3 ubiquitin-protein ligase RNF144A;
DE            EC=6.3.2.-;
DE   AltName: Full=RING finger protein 144A;
DE   AltName: Full=UbcM4-interacting protein 4;
DE   AltName: Full=Ubiquitin-conjugating enzyme 7-interacting protein 4;
GN   Name=RNF144A; Synonyms=KIAA0161, RNF144, UBCE7IP4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ALA-4.
RC   TISSUE=Bone marrow;
RX   MEDLINE=96281124; PubMed=8724849; DOI=10.1093/dnares/3.1.17;
RA   Nagase T., Seki N., Ishikawa K., Tanaka A., Nomura N.;
RT   "Prediction of the coding sequences of unidentified human genes. V.
RT   The coding sequences of 40 new genes (KIAA0161-KIAA0200) deduced by
RT   analysis of cDNA clones from human cell line KG-1.";
RL   DNA Res. 3:17-24(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H.,
RA   Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M.,
RA   Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E.,
RA   Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J.,
RA   Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C.,
RA   Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J.,
RA   Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A.,
RA   Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K.,
RA   Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M.,
RA   Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N.,
RA   Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M.,
RA   Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E.,
RA   Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P.,
RA   Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A.,
RA   Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A.,
RA   Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T.,
RA   Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D.,
RA   Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X.,
RA   McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D.,
RA   Waterston R.H., Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2
RT   and 4.";
RL   Nature 434:724-731(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ALA-4.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ALA-4.
RC   TISSUE=Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   INTERACTION WITH UBE2L3.
RX   MEDLINE=99358765; PubMed=10431818; DOI=10.1016/S0014-5793(99)00823-6;
RA   Martinez-Noel G., Niedenthal R., Tamura T., Harbers K.;
RT   "A family of structurally related RING finger proteins interacts
RT   specifically with the ubiquitin-conjugating enzyme UbcM4.";
RL   FEBS Lett. 454:257-261(1999).
RN   [6]
RP   STRUCTURE BY NMR OF 20-100.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the RING finger domain of the human UbcM4-
RT   interacting protein 4.";
RL   Submitted (NOV-2004) to the PDB data bank.
CC   -!- FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from
CC       E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of
CC       a thioester and then directly transfers the ubiquitin to targeted
CC       substrates (By similarity).
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Interacts with UBE2L3.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- SIMILARITY: Belongs to the RBR family. RNF144 subfamily.
CC   -!- SIMILARITY: Contains 1 IBR-type zinc finger.
CC   -!- SIMILARITY: Contains 2 RING-type zinc fingers.
CC   -!- CAUTION: Lacks the His residue in the RING-type domain 2 that is
CC       one of the conserved features of the family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA11478.2; Type=Erroneous initiation;
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DR   EMBL; D79983; BAA11478.2; ALT_INIT; mRNA.
DR   EMBL; AC068481; AAX82010.1; -; Genomic_DNA.
DR   EMBL; CH471053; EAX01030.1; -; Genomic_DNA.
DR   EMBL; CH471053; EAX01031.1; -; Genomic_DNA.
DR   EMBL; BC050373; AAH50373.1; -; mRNA.
DR   IPI; IPI00220842; -.
DR   RefSeq; NP_055561.2; NM_014746.3.
DR   UniGene; Hs.22146; -.
DR   PDB; 1WIM; NMR; -; A=20-100.
DR   PDBsum; 1WIM; -.
DR   ProteinModelPortal; P50876; -.
DR   SMR; P50876; 20-157, 179-230.
DR   IntAct; P50876; 6.
DR   STRING; P50876; -.
DR   PRIDE; P50876; -.
DR   Ensembl; ENST00000320892; ENSP00000321330; ENSG00000151692.
DR   GeneID; 9781; -.
DR   KEGG; hsa:9781; -.
DR   UCSC; uc002qys.1; human.
DR   CTD; 9781; -.
DR   GeneCards; GC02P006974; -.
DR   HGNC; HGNC:20457; RNF144A.
DR   neXtProt; NX_P50876; -.
DR   PharmGKB; PA162401542; -.
DR   eggNOG; prNOG07763; -.
DR   GeneTree; ENSGT00550000074424; -.
DR   HOGENOM; HBG314219; -.
DR   HOVERGEN; HBG052072; -.
DR   InParanoid; P50876; -.
DR   OMA; CKCSKGD; -.
DR   OrthoDB; EOG4WWRK1; -.
DR   PhylomeDB; P50876; -.
DR   NextBio; 36828; -.
DR   ArrayExpress; P50876; -.
DR   Bgee; P50876; -.
DR   CleanEx; HS_RNF144A; -.
DR   Genevestigator; P50876; -.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   InterPro; IPR002867; Znf_C6HC.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR017907; Znf_RING_CS.
DR   Pfam; PF01485; IBR; 2.
DR   SMART; SM00647; IBR; 2.
DR   SMART; SM00184; RING; 2.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Ligase; Membrane; Metal-binding;
KW   Polymorphism; Reference proteome; Repeat; Transmembrane;
KW   Transmembrane helix; Ubl conjugation pathway; Zinc; Zinc-finger.
FT   CHAIN         1    292       Probable E3 ubiquitin-protein ligase
FT                                RNF144A.
FT                                /FTId=PRO_0000056298.
FT   TRANSMEM    250    270       Helical; (Potential).
FT   ZN_FING      20     70       RING-type 1; atypical.
FT   ZN_FING      91    156       IBR-type.
FT   ZN_FING     185    214       RING-type 2; degenerate.
FT   VARIANT       4      4       T -> A (in dbSNP:rs364891).
FT                                /FTId=VAR_035375.
FT   STRAND       21     23
FT   HELIX        29     31
FT   STRAND       32     35
FT   TURN         36     39
FT   STRAND       40     43
FT   HELIX        44     57
FT   HELIX        78     84
FT   HELIX        87     99
SQ   SEQUENCE   292 AA;  32890 MW;  E5BE7BC9560DCF93 CRC64;
     MTTTRYRPTW DLALDPLVSC KLCLGEYPVE QMTTIAQCQC IFCTLCLKQY VELLIKEGLE
     TAISCPDAAC PKQGHLQENE IECMVAAEIM QRYKKLQFER EVLFDPCRTW CPASTCQAVC
     QLQDVGLQTP QPVQCKACRM EFCSTCKASW HPGQGCPETM PITFLPGETS AAFKMEEDDA
     PIKRCPKCKV YIERDEGCAQ MMCKNCKHAF CWYCLESLDD DFLLIHYDKG PCRNKLGHSR
     ASVIWHRTQV VGIFAGFGLL LLVASPFLLL ATPFVLCCKC KCSKGDDDPL PT
//