ID ERF_HUMAN Reviewed; 548 AA. AC P50548; Q9UPI7; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 19-JUL-2004, sequence version 2. DT 20-FEB-2007, entry version 60. DE ETS domain-containing transcription factor ERF (Ets2 repressor DE factor). GN Name=ERF; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX MEDLINE=96030784; PubMed=7588608; RA Sgouras D.N., Athanasiou M.A., Beal G.J. Jr., Fisher R.J., Blair D.G., RA Mavrothalassitis G.J.; RT "ERF: an ETS domain protein with strong transcriptional repressor RT activity, can suppress ets-associated tumorigenesis and is regulated RT by phosphorylation during cell cycle and mitogenic stimulation."; RL EMBO J. 14:4781-4793(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=15057824; DOI=10.1038/nature02399; RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., RA Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., RA Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., RA Caenepeel S., Carrano A.V., Caoile C., Chan Y.M., Christensen M., RA Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., RA Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., RA Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., RA Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., RA Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., RA Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., RA Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., RA Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., RA Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., RA Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., RA Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., RA Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., RA Rubin E.M., Lucas S.M.; RT "The DNA sequence and biology of human chromosome 19."; RL Nature 428:529-535(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Duodenum; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-185; SER-187; RP SER-190 AND SER-531, AND MASS SPECTROMETRY. RX PubMed=17081983; DOI=10.1016/j.cell.2006.09.026; RA Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., RA Mann M.; RT "Global, in vivo, and site-specific phosphorylation dynamics in RT signaling networks."; RL Cell 127:635-648(2006). RN [5] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-526, AND MASS RP SPECTROMETRY. RX PubMed=16964243; DOI=10.1038/nbt1240; RA Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; RT "A probability-based approach for high-throughput protein RT phosphorylation analysis and site localization."; RL Nat. Biotechnol. 24:1285-1292(2006). CC -!- FUNCTION: Potent transcriptional repressor that binds to the H1 CC element of the Ets2 promoter. May regulate other genes involved in CC cellular proliferation. CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- PTM: Phosphorylated by multiple kinases including probably ERK2. CC Phosphorylation regulates the activity of ERF. CC -!- SIMILARITY: Belongs to the ETS family. CC -!- SIMILARITY: Contains 1 ETS DNA-binding domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U15655; AAA86686.1; -; mRNA. DR EMBL; AC006486; AAD11987.1; -; Genomic_DNA. DR EMBL; BC022231; AAH22231.1; -; mRNA. DR PIR; S59133; S59133. DR UniGene; Hs.466828; -. DR HSSP; Q01543; 1FLI. DR TRANSFAC; T04885; -. DR Ensembl; ENSG00000105722; Homo sapiens. DR KEGG; hsa:2077; -. DR H-InvDB; HIX0015174; -. DR HGNC; HGNC:3444; ERF. DR ArrayExpress; P50548; -. DR GermOnline; ENSG00000105722; Homo sapiens. DR RZPD-ProtExp; IOH10619; -. DR RZPD-ProtExp; S0501; -. DR GO; GO:0003706; F:ligand-regulated transcription factor activity; TAS:ProtInc. DR GO; GO:0003714; F:transcription corepressor activity; TAS:ProtInc. DR GO; GO:0008283; P:cell proliferation; TAS:ProtInc. DR GO; GO:0000074; P:regulation of progression through cell cycle; TAS:ProtInc. DR GO; GO:0006357; P:regulation of transcription from RNA polyme...; TAS:ProtInc. DR InterPro; IPR000418; Ets. DR InterPro; IPR011071; Lyase_8_like_C. DR InterPro; IPR011991; Wing_hlx_DNA_bd. DR Gene3D; G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1. DR Pfam; PF00178; Ets; 1. DR PRINTS; PR00454; ETSDOMAIN. DR SMART; SM00413; ETS; 1. DR PROSITE; PS00345; ETS_DOMAIN_1; 1. DR PROSITE; PS00346; ETS_DOMAIN_2; 1. DR PROSITE; PS50061; ETS_DOMAIN_3; 1. KW DNA-binding; Nuclear protein; Phosphorylation; Repressor; KW Transcription; Transcription regulation. FT CHAIN 1 548 ETS domain-containing transcription FT factor ERF. FT /FTId=PRO_0000204101. FT DNA_BIND 27 107 ETS. FT COMPBIAS 166 171 Poly-Ser. FT COMPBIAS 290 293 Poly-Gly. FT COMPBIAS 362 373 Poly-Ser. FT COMPBIAS 418 423 Poly-Pro. FT COMPBIAS 496 499 Poly-Gly. FT MOD_RES 21 21 Phosphoserine. FT MOD_RES 185 185 Phosphoserine. FT MOD_RES 187 187 Phosphoserine. FT MOD_RES 190 190 Phosphoserine. FT MOD_RES 526 526 Phosphothreonine (by MAPK1). FT MOD_RES 531 531 Phosphoserine. FT MUTAGEN 526 526 T->A: Loss of a phosphorylation site. FT CONFLICT 381 381 P -> R (in Ref. 1). FT CONFLICT 398 398 G -> A (in Ref. 1). SQ SEQUENCE 548 AA; 58703 MW; 01242339B8D328ED CRC64; MKTPADTGFA FPDWAYKPES SPGSRQIQLW HFILELLRKE EYQGVIAWQG DYGEFVIKDP DEVARLWGVR KCKPQMNYDK LSRALRYYYN KRILHKTKGK RFTYKFNFNK LVLVNYPFID VGLAGGAVPQ SAPPVPSGGS HFRFPPSTPS EVLSPTEDPR SPPACSSSSS SLFSAVVARR LGRGSVSDCS DGTSELEEPL GEDPRARPPG PPDLGAFRGP PLARLPHDPG VFRVYPRPRG GPEPLSPFPV SPLAGPGSLL PPQLSPALPM TPTHLAYTPS PTLSPMYPSG GGGPSGSGGG SHFSFSPEDM KRYLQAHTQS VYNYHLSPRA FLHYPGLVVP QPQRPDKCPL PPMAPETPPV PSSASSSSSS SSSPFKFKLQ PPPLGRRQRA AGEKAVAGAD KSGGSAGGLA EGAGALAPPP PPPQIKVEPI SEGESEEVEV TDISDEDEED GEVFKTPRAP PAPPKPEPGE APGASQCMPL KLRFKRRWSE DCRLEGGGGP AGGFEDEGED KKVRGEGPGE AGGPLTPRRV SSDLQHATAQ LSLEHRDS //