ID CLK1_HUMAN STANDARD; PRT; 484 AA. AC P49759; DT 01-OCT-1996 (Rel. 34, Created) DT 01-OCT-1996 (Rel. 34, Last sequence update) DT 01-FEB-2005 (Rel. 46, Last annotation update) DE Dual specificity protein kinase CLK1 (EC 2.7.1.37) (EC 2.7.1.112) (CDC DE like kinase 1). GN Name=CLK1; Synonyms=CLK; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=91139618; PubMed=1704889; RA Johnson K.W., Smith K.A.; RT "Molecular cloning of a novel human cdc2/CDC28-like protein kinase."; RL J. Biol. Chem. 266:3402-3407(1991). RN [2] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=95082033; PubMed=7990150; RA Hanes J.J., der Kammer H., Klaudiny J.J., Scheit K.H.; RT "Characterization by cDNA cloning of two new human protein kinases. RT Evidence by sequence comparison of a new family of mammalian protein RT kinases."; RL J. Mol. Biol. 244:665-672(1994). RN [3] RP SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S). RX PubMed=14759258; DOI=10.1186/gb-2004-5-2-r8; RA Hillman R.T., Green R.E., Brenner S.E.; RT "An unappreciated role for RNA surveillance."; RL Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004). CC -!- FUNCTION: Phosphorylates serine- and arginine-rich (SR) proteins CC of the spliceosomal complex may be a constituent of a network of CC regulatory mechanisms that enable SR proteins to control RNA CC splicing. Phosphorylates serines, threonines and tyrosines (By CC similarity). CC -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. CC -!- CATALYTIC ACTIVITY: ATP + a protein tyrosine = ADP + a protein CC tyrosine phosphate. CC -!- SUBUNIT: Interacts with PPIG. CC -!- SUBCELLULAR LOCATION: Nuclear. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=Long; CC IsoId=P49759-1; Sequence=Displayed; CC Name=Short; CC IsoId=P49759-2; Sequence=VSP_004852, VSP_004853; CC Note=Lacks the kinase domain. May be produced at very low levels CC due to a premature stop codon in the mRNA, leading to CC nonsense-mediated mRNA decay; CC -!- PTM: Autophosphorylates on all three types of residues (By CC similarity). CC -!- SIMILARITY: Belongs to the Ser/Thr protein kinase family. Lammer CC subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L29219; AAA61480.1; -. DR EMBL; L29222; AAB59459.1; -. DR PIR; S53641; S53641. DR HSSP; Q00534; 1BI8. DR Ensembl; ENSG00000013441; Homo sapiens. DR Genew; HGNC:2068; CLK1. DR H-InvDB; HIX0002728; -. DR MIM; 601951; -. DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kina...; TAS. DR GO; GO:0004674; F:protein serine/threonine kinase activity; TAS. DR GO; GO:0008283; P:cell proliferation; TAS. DR GO; GO:0000074; P:regulation of cell cycle; TAS. DR InterPro; IPR011009; Kinase_like. DR InterPro; IPR000719; Prot_kinase. DR InterPro; IPR008271; Ser_thr_pkin_AS. DR InterPro; IPR002290; Ser_thr_pkinase. DR Pfam; PF00069; Pkinase; 1. DR ProDom; PD000001; Prot_kinase; 1. DR SMART; SM00220; S_TKc; 1. DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1. DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1. DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1. KW Alternative splicing; ATP-binding; Nuclear protein; Phosphorylation; KW Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. FT DOMAIN 161 477 Protein kinase. FT NP_BIND 167 175 ATP (By similarity). FT BINDING 191 191 ATP (By similarity). FT ACT_SITE 288 288 Proton acceptor (By similarity). FT VARSPLIC 131 136 KSHRRK -> MKLLIL (in isoform Short). FT /FTId=VSP_004852. FT VARSPLIC 137 484 Missing (in isoform Short). FT /FTId=VSP_004853. SQ SEQUENCE 484 AA; 57205 MW; 304B5B4486AD0A6B CRC64; MRHSKRTYCP DWDDKDWDYG KWRSSSSHKR RKRSHSSAQE NKRCKYNHSK MCDSHYLESR SINEKDYHSR RYIDEYRNDY TQGCEPGHRQ RDHESRYQNH SSKSSGRSGR SSYKSKHRIH HSTSHRRSHG KSHRRKRTRS VEDDEEGHLI CQSGDVLSAR YEIVDTLGEG AFGKVVECID HKAGGRHVAV KIVKNVDRYC EAARSEIQVL EHLNTTDPNS TFRCVQMLEW FEHHGHICIV FELLGLSTYD FIKENGFLPF RLDHIRKMAY QICKSVNFLH SNKLTHTDLK PENILFVQSD YTEAYNPKIK RDERTLINPD IKVVDFGSAT YDDEHHSTLV STRHYRAPEV ILALGWSQPC DVWSIGCILI EYYLGFTVFP THDSKEHLAM MERILGPLPK HMIQKTRKRK YFHHDRLDWD EHSSAGRYVS RACKPLKEFM LSQDVEHERL FDLIQKMLEY DPAKRITLRE ALKHPFFDLL KKSI //