ID SNG1_YEAST Reviewed; 547 AA. AC P46950; D6VUX9; DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1995, sequence version 1. DT 03-MAY-2011, entry version 78. DE RecName: Full=Nitrosoguanidine resistance protein SNG1; GN Name=SNG1; OrderedLocusNames=YGR197C; ORFNames=G7591; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=95272615; PubMed=7753113; DOI=10.1016/0165-7992(95)90037-3; RA Grey M., Pich C.T., Haase E., Brendel M.; RT "SNG1 -- a new gene involved in nitrosoguanidine resistance in RT Saccharomyces cerevisiae."; RL Mutat. Res. 346:207-214(1995). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=96076633; PubMed=7502584; DOI=10.1002/yea.320111110; RA Guerreiro P., Maia e Silva A., Barreiros T., Arroyo J., RA Garcia-Gonzalez M., Garcia-Saez M.I., Rodrigues-Pousada C., RA Nombela C.; RT "The complete sequence of a 9000 bp fragment of the right arm of RT Saccharomyces cerevisiae chromosome VII contains four previously RT unknown open reading frames."; RL Yeast 11:1087-1091(1995). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=97313265; PubMed=9169869; RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., RA Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., RA Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., RA Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., RA Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., RA Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., RA Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., RA Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., RA Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., RA Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., RA Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., RA Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., RA Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., RA Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., RA Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., RA Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., RA Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., RA Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., RA Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., RA van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., RA Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., RA Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., RA Zollner A., Kleine K.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."; RL Nature 387:81-84(1997). RN [4] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases. RN [5] RP INDUCTION. RX PubMed=12529331; DOI=10.1074/jbc.M208549200; RA Hikkel I., Lucau-Danila A., Delaveau T., Marc P., Devaux F., Jacq C.; RT "A general strategy to uncover transcription factor properties RT identifies a new regulator of drug resistance in yeast."; RL J. Biol. Chem. 278:11427-11432(2003). RN [6] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91, AND MASS RP SPECTROMETRY. RC STRAIN=ADR376; RX PubMed=17330950; DOI=10.1021/pr060559j; RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., RA Elias J.E., Gygi S.P.; RT "Large-scale phosphorylation analysis of alpha-factor-arrested RT Saccharomyces cerevisiae."; RL J. Proteome Res. 6:1190-1197(2007). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45 AND THR-91, AND MASS RP SPECTROMETRY. RX PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200; RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; RT "A multidimensional chromatography technology for in-depth RT phosphoproteome analysis."; RL Mol. Cell. Proteomics 7:1389-1396(2008). CC -!- FUNCTION: May function as a N-methyl-N'nitro-N-nitrosoguanidine CC (MNNG) export permease. CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein CC (Potential). CC -!- INDUCTION: Transcriptionally regulated by PDR8. CC -!- SIMILARITY: To yeast YJR015W. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X74920; CAA52881.1; -; Genomic_DNA. DR EMBL; X82775; CAA58016.1; -; Genomic_DNA. DR EMBL; Z72982; CAA97224.1; -; Genomic_DNA. DR EMBL; BK006941; DAA08290.1; -; Genomic_DNA. DR PIR; S53920; S53920. DR RefSeq; NP_011713.1; NM_001181326.1. DR ProteinModelPortal; P46950; -. DR DIP; DIP-8148N; -. DR IntAct; P46950; 1. DR STRING; P46950; -. DR EnsemblFungi; YGR197C; YGR197C; YGR197C. DR GeneID; 853111; -. DR KEGG; sce:YGR197C; -. DR NMPDR; fig|4932.3.peg.2840; -. DR CYGD; YGR197c; -. DR SGD; S000003429; SNG1. DR eggNOG; fuNOG04192; -. DR GeneTree; EFGT00050000006391; -. DR HOGENOM; HBG204421; -. DR OMA; KSGFLVY; -. DR OrthoDB; EOG4TF3V7; -. DR PhylomeDB; P46950; -. DR NextBio; 973127; -. DR ArrayExpress; P46950; -. DR Genevestigator; P46950; -. DR GermOnline; YGR197C; Saccharomyces cerevisiae. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IDA:SGD. DR GO; GO:0015931; P:nucleobase, nucleoside, nucleotide and nucleic acid transport; IMP:SGD. DR InterPro; IPR022703; DUF3533. DR Pfam; PF12051; DUF3533; 1. PE 1: Evidence at protein level; KW Complete proteome; Membrane; Phosphoprotein; Transmembrane; KW Transmembrane helix. FT CHAIN 1 547 Nitrosoguanidine resistance protein SNG1. FT /FTId=PRO_0000072007. FT TRANSMEM 109 129 Helical; (Potential). FT TRANSMEM 159 179 Helical; (Potential). FT TRANSMEM 318 338 Helical; (Potential). FT TRANSMEM 363 383 Helical; (Potential). FT TRANSMEM 394 414 Helical; (Potential). FT TRANSMEM 418 438 Helical; (Potential). FT TRANSMEM 457 477 Helical; (Potential). FT TRANSMEM 488 508 Helical; (Potential). FT MOD_RES 45 45 Phosphoserine. FT MOD_RES 91 91 Phosphothreonine. FT CONFLICT 117 117 A -> V (in Ref. 1; CAA52881). SQ SEQUENCE 547 AA; 61293 MW; D4421BA2DED55420 CRC64; MTKSVGDEES QYIEDPSFAA AAAFTGGRDG VSYSNQRFAE GSGHSSDLAK SLEDYRPPDE KPSSLSSVGE GGANEEEKGG NDGGPLARIQ TGLFSPRLRN HRKKILSKFV LNNFFIACVC VSLISIYWGA CYGTDRYFFK VKNIVVLQDA PSNTSVQSIS AIIPSLLASV PGTWHIYNAT SFHRKFGTTN STEIDRKIVD LIYDERYWLA LNVKPNATDT LYNSLISQDA NSEFNSSIFF ESVFESGRDP SSVKSTILPL MQQLEVRLQK YYVKEYLPSL MSNITSNDRD LNINMENWAI AGQLLFTYND YRPFADRILM APLQVGLIYC ILLTVLQLSL YGKLHGEMAR VLKPKHILIY RLLISWATYF LLSIGFCTVS AIFRIDFTPA FGRGGFVVYW MSTWLVMMAV GGANENVLSL VIAYCPPYLS IWLMTWIILN ISASFYPMVL NNEFYRYGYI MPIHNAVDIY KVIFLNLTKR KMGRNYGILV AWVALNTSLM PFCMKFAGKK MQKNAMQAAE AAVAAATQRA SRPAEANTDK NNNPPGN //