ID PURK_MYCLE Reviewed; 439 AA. AC P46701; DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1995, sequence version 1. DT 03-APR-2007, entry version 45. DE Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21) DE (AIR carboxylase) (AIRC). GN Name=purK; OrderedLocusNames=ML0735; ORFNames=B1308_F1_32; OS Mycobacterium leprae. OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Mycobacteriaceae; Mycobacterium. OX NCBI_TaxID=1769; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=96084954; PubMed=7489918; DOI=10.1016/0378-1119(95)00427-8; RA Doukhan L., Predich M., Nair G., Dussurget O., Mandic-Mulec I., RA Cole S.T., Smith D.R., Smith I.; RT "Genomic organization of the mycobacterial sigma gene cluster."; RL Gene 165:67-70(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=TN; RX MEDLINE=21128732; PubMed=11234002; DOI=10.1038/35059006; RA Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., RA Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., RA Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., RA Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N., RA Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., RA Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., RA Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., RA Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., RA Barrell B.G.; RT "Massive gene decay in the leprosy bacillus."; RL Nature 409:1007-1011(2001). CC -!- FUNCTION: Possesses an ATPase activity that is dependent on the CC presence of AIR (aminoimidazole ribonucleotide). The association CC of purK and purE produces an enzyme complex capable of converting CC AIR to CAIR efficiently under physiological condition (By CC similarity). CC -!- CATALYTIC ACTIVITY: 5-amino-1-(5-phospho-D-ribosyl)imidazole-4- CC carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2). CC -!- PATHWAY: Nucleotide biosynthesis; IMP biosynthesis; 5-amino-1-(5- CC phospho-D-ribosyl)imidazole-4-carboxamide from 2-N-formyl-1-N-(5- CC phospho-D-ribosyl)glycinamide: step 3/5. CC -!- PATHWAY: Context: Purine biosynthesis. CC -!- SUBUNIT: Homodimer (By similarity). CC -!- SIMILARITY: Belongs to the purK/purT family. CC -!- SIMILARITY: Contains 1 ATP-grasp domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U00012; AAA85935.1; -; Genomic_DNA. DR EMBL; AL583919; CAC30244.1; -; Genomic_DNA. DR PIR; H87000; H87000. DR HSSP; P09029; 1B6R. DR GenomeReviews; AL450380_GR; ML0735. DR KEGG; mle:ML0735; -. DR Leproma; ML0735; -. DR BioCyc; MLEP1769:ML0735-MONOMER; -. DR InterPro; IPR011761; ATP-grasp. DR InterPro; IPR003135; ATP-grasp_carboxylate-amine. DR InterPro; IPR013816; ATP_grasp_subdomain_2. DR InterPro; IPR013817; Pre-ATP_grasp. DR InterPro; IPR005875; PurK_ATP. DR InterPro; IPR011054; Rudmnt_hyb_motif. DR Pfam; PF02222; ATP-grasp; 1. DR TIGRFAMs; TIGR01161; purK; 1. DR PROSITE; PS50975; ATP_GRASP; 1. KW ATP-binding; Complete proteome; Decarboxylase; Lyase; KW Nucleotide-binding; Purine biosynthesis. FT CHAIN 1 439 Phosphoribosylaminoimidazole carboxylase FT ATPase subunit. FT /FTId=PRO_0000074999. FT DOMAIN 117 313 ATP-grasp. FT NP_BIND 197 200 ATP (By similarity). FT NP_BIND 283 284 ATP (By similarity). FT BINDING 113 113 ATP (By similarity). FT BINDING 160 160 ATP (By similarity). FT BINDING 205 205 ATP (By similarity). SQ SEQUENCE 439 AA; 47306 MW; 288C5DD5EE7508EE CRC64; MMAVPSRCSL GVAPLVAMVG GGQLARMTHQ AAIALGQTLR VLATAADEPA AQVTPDVVIG SHTDLEDLRR VALGADALTF DHEHVPTELL DKLVAEGINV APSPQALVHA QDKLVMRRRL AALGAAMPRF MALDSVDDLA EIDAFAQRLT GSKDAPMVVK AVRGGYDGRG VQMVRDSAHA REVASGYLVD GMPVLVEERV ELRRELSALV ARSPFGQGAA WPVVETVQRD GICVLVVAPA LALADDLASA AQQLALRLAA ELGVVGVFAV ELFETADGAL LVNELAMRPH NSGHWTMDGA RTSQFEQHLR AVLDYPLGET DAVAPVTVMV NVLGAPQPPT LSVVTMDERL HHLFARMPDA RVHLYDKVER PGRKVGHINF RGTDKDRKNP TKLRERAELA AHWLSHGQWT DGWDPHRAGD DVVEISLACG GRNDAQRQR //