ID CAS2_ECOLI Reviewed; 94 AA. AC P45956; Q2MA75; DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot. DT 30-MAY-2000, sequence version 2. DT 06-MAR-2013, entry version 79. DE RecName: Full=CRISPR-associated endoribonuclease Cas2; DE EC=3.1.-.-; GN Name=ygbF; Synonyms=cas2; OrderedLocusNames=b2754, JW5438; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12; RX MEDLINE=89255132; PubMed=2656660; RA Nakata A., Amemura M., Makino K.; RT "Unusual nucleotide arrangement with repeated sequences in the RT Escherichia coli K-12 chromosome."; RL J. Bacteriol. 171:3553-3556(1989). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [4] RP IDENTIFICATION. RX MEDLINE=96032851; PubMed=7567469; DOI=10.1093/nar/23.17.3554; RA Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., RA Danchin A.; RT "Detection of new genes in a bacterial genome using Markov models for RT three gene classes."; RL Nucleic Acids Res. 23:3554-3562(1995). RN [5] RP OPERON STRUCTURE, AND INDUCTION BY LEUO. RC STRAIN=K12 / BW25113; RX PubMed=19429622; DOI=10.1128/JB.00108-09; RA Shimada T., Yamamoto K., Ishihama A.; RT "Involvement of the leucine response transcription factor LeuO in RT regulation of the genes for sulfa drug efflux."; RL J. Bacteriol. 191:4562-4571(2009). RN [6] RP INDUCTION BY H-NS. RC STRAIN=K12; RX PubMed=20132443; DOI=10.1111/j.1365-2958.2010.07073.x; RA Pul U., Wurm R., Arslan Z., Geissen R., Hofmann N., Wagner R.; RT "Identification and characterization of E. coli CRISPR-cas promoters RT and their silencing by H-NS."; RL Mol. Microbiol. 75:1495-1512(2010). RN [7] RP INDUCTION BY BAER, ROLE IN PLASMID SILENCING, AND DISRUPTION RP PHENOTYPE. RC STRAIN=K12 / BW25113; RX PubMed=21255106; DOI=10.1111/j.1365-2958.2010.07482.x; RA Perez-Rodriguez R., Haitjema C., Huang Q., Nam K.H., Bernardis S., RA Ke A., DeLisa M.P.; RT "Envelope stress is a trigger of CRISPR RNA-mediated DNA silencing in RT Escherichia coli."; RL Mol. Microbiol. 79:584-599(2011). CC -!- FUNCTION: CRISPR (clustered regularly interspaced short CC palindromic repeat), is an adaptive immune system that provides CC protection against mobile genetic elements (viruses, transposable CC elements and conjugative plasmids). CRISPR clusters contain CC sequences complementary to antecedent mobile elements and target CC invading nucleic acids. CRISPR clusters are transcribed and CC processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific CC endoribonuclease (By similarity). CC -!- INDUCTION: Repressed by H-NS, activated by LeuO. Activated by the CC BaeSR two-component regulatory system, possibly due to envelope CC stress. Part of the casABCDE-ygbT-ygbF operon. CC -!- DISRUPTION PHENOTYPE: Loss of plasmid silencing. CC -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2 CC protein family. E.coli-subtype subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M27059; -; NOT_ANNOTATED_CDS; Unassigned_DNA. DR EMBL; U29579; AAA69264.1; -; Genomic_DNA. DR EMBL; U00096; AAC75796.2; -; Genomic_DNA. DR EMBL; AP009048; BAE76831.1; -; Genomic_DNA. DR PIR; F65056; F65056. DR RefSeq; NP_417234.2; NC_000913.2. DR RefSeq; YP_490963.1; NC_007779.1. DR ProteinModelPortal; P45956; -. DR EnsemblBacteria; EBESCT00000004828; EBESCP00000004828; EBESCG00000003942. DR EnsemblBacteria; EBESCT00000017872; EBESCP00000017163; EBESCG00000016928. DR GeneID; 12930591; -. DR GeneID; 947213; -. DR GenomeReviews; AP009048_GR; JW5438. DR GenomeReviews; U00096_GR; b2754. DR KEGG; ecj:Y75_p2692; -. DR KEGG; eco:b2754; -. DR PATRIC; 32120918; VBIEscCol129921_2851. DR EchoBASE; EB2694; -. DR EcoGene; EG12845; ygbF. DR eggNOG; NOG12609; -. DR HOGENOM; HOG000015873; -. DR OMA; MLVIVVE; -. DR ProtClustDB; PRK11558; -. DR BioCyc; EcoCyc:EG12845-MONOMER; -. DR BioCyc; ECOL316407:JW5438-MONOMER; -. DR Genevestigator; P45956; -. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW. DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IMP:EcoCyc. DR GO; GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. DR InterPro; IPR010152; CRISPR-assoc_prot_Cas2_sub. DR Pfam; PF09707; Cas_Cas2CT1978; 1. DR TIGRFAMs; TIGR01873; cas_CT1978; 1. PE 2: Evidence at transcript level; KW Antiviral defense; Complete proteome; Endonuclease; Hydrolase; KW Nuclease; Reference proteome. FT CHAIN 1 94 CRISPR-associated endoribonuclease Cas2. FT /FTId=PRO_0000169312. SQ SEQUENCE 94 AA; 10518 MW; D1C159D924B477B4 CRC64; MSMLVVVTEN VPPRLRGRLA IWLLEVRAGV YVGDVSAKIR EMIWEQIAGL AEEGNVVMAW ATNTETGFEF QTFGLNRRTP VDLDGLRLVS FLPV //