ID GCN20_YEAST STANDARD; PRT; 752 AA. AC P43535; DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1995, sequence version 1. DT 13-JUN-2006, entry version 52. DE Protein GCN20. GN Name=GCN20; OrderedLocusNames=YFR009W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=95347344; PubMed=7621831; RA Vazquez de Aldana C.R., Marton M.J., Hinnebusch A.G.; RT "GCN20, a novel ATP binding cassette protein, and GCN1 reside in a RT complex that mediates activation of the eIF-2 alpha kinase GCN2 in RT amino acid-starved cells."; RL EMBO J. 14:3184-3199(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S288c / AB972; RX MEDLINE=95400292; PubMed=7670463; DOI=10.1038/ng0795-261; RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., RA Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., RA Yamazaki M., Tashiro H., Eki T.; RT "Analysis of the nucleotide sequence of chromosome VI from RT Saccharomyces cerevisiae."; RL Nat. Genet. 10:261-268(1995). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=S288c; RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., RA LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces RT cerevisiae ORFs in the Gateway recombinational cloning system."; RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases. RN [4] RP LEVEL OF PROTEIN EXPRESSION. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). CC -!- FUNCTION: Positive effector of the eIF-2-alpha kinase activity of CC GCN2. CC -!- SUBUNIT: Component of a heteromeric complex that includes GCN1 and CC GCN20. CC -!- MISCELLANEOUS: Present with 14600 molecules/cell. CC -!- SIMILARITY: Belongs to the ABC transporter family. EF3 subfamily. CC -!- SIMILARITY: Contains 2 ABC transporter domains. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U19971; AAA75444.1; -; Genomic_DNA. DR EMBL; D50617; BAA09248.1; -; Genomic_DNA. DR EMBL; AY723804; AAU09721.1; -; Genomic_DNA. DR PIR; S56146; S56146. DR IntAct; P43535; -. DR GermOnline; 140163; -. DR Ensembl; YFR009W; Saccharomyces cerevisiae. DR GenomeReviews; D50617_GR; YFR009W. DR SGD; S000001905; GCN20. DR BioCyc; SCER-S28-01:SCER-S28-01-001948-MONOMER; -. DR LinkHub; P43535; -. DR GO; GO:0005830; C:cytosolic ribosome (sensu Eukaryota); IDA. DR GO; GO:0006448; P:regulation of translational elongation; IMP. DR InterPro; IPR003593; AAA_ATPase. DR InterPro; IPR003439; ABC_transp_like. DR Pfam; PF00005; ABC_tran; 2. DR ProDom; PD000006; ABC_transporter; 2. DR SMART; SM00382; AAA; 2. DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1. DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2. KW ATP-binding; Complete proteome; Nucleotide-binding; Repeat. FT CHAIN 1 752 Protein GCN20. FT /FTId=PRO_0000093461. FT DOMAIN 199 464 ABC transporter 1. FT DOMAIN 532 748 ABC transporter 2. FT NP_BIND 232 239 ATP 1 (Potential). FT NP_BIND 565 572 ATP 2 (Potential). SQ SEQUENCE 752 AA; 85027 MW; 486FED10305A572E CRC64; MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ FVSDLLIDAG ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD INVLKSHNSK SDINVSLSML GVNGDIEHTG RKMETRVDLK KLAKAEQKIA KKVAKRNNKF VKYEASKLIN DQKEEDYDSF FLQINPLEFG SSAGKSKDIH IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST LLRALSRREL NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI LYGLGFSTEA QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV PSIAYLAEYL KTYPNTVLTV SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF DTFYTTKEER RKNAQREYDN QMVYRKHLQE FIDKYRYNAA KSQEAQSRIK KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG YDENNLLLKD VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL LSGGQKSRVA FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV LMVSHDISVI DSVCKEIWVS EQGTVKRFEG TIYDYRDYIL QSADAAGVVK KH //