ID GCN20_YEAST STANDARD; PRT; 752 AA. AC P43535; DT 01-NOV-1995 (Rel. 32, Created) DT 01-NOV-1995 (Rel. 32, Last sequence update) DT 01-FEB-2005 (Rel. 46, Last annotation update) DE GCN20 protein. GN Name=GCN20; OrderedLocusNames=YFR009W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE. RX MEDLINE=95347344; PubMed=7621831; RA Vazquez de Aldana C.R., Marton M.J., Hinnebusch A.G.; RT "GCN20, a novel ATP binding cassette protein, and GCN1 reside in a RT complex that mediates activation of the eIF-2 alpha kinase GCN2 in RT amino acid-starved cells."; RL EMBO J. 14:3184-3199(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S288c / AB972; RX MEDLINE=95400292; PubMed=7670463; RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., RA Sasanuma S.-I., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., RA Yamazaki M.-A., Tashiro H., Eki T.; RT "Analysis of the nucleotide sequence of chromosome VI from RT Saccharomyces cerevisiae."; RL Nat. Genet. 10:261-268(1995). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=S288c; RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., RA LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces RT cerevisiae ORFs in the Gateway recombinational cloning system."; RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Positive effector of the eIF-2-alpha kinase activity of CC GCN2. CC -!- SUBUNIT: Component of a heteromeric complex that includes GCN1 and CC GCN20. CC -!- SIMILARITY: Belongs to the ABC transporter family. EF3 subfamily. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U19971; AAA75444.1; -. DR EMBL; D50617; BAA09248.1; -. DR EMBL; AY723804; AAU09721.1; -. DR PIR; S56146; S56146. DR IntAct; P43535; -. DR GermOnline; 140163; -. DR SGD; S000001905; GCN20. DR GO; GO:0005830; C:cytosolic ribosome (sensu Eukaryota); IDA. DR GO; GO:0006448; P:regulation of translational elongation; IMP. DR InterPro; IPR003593; AAA_ATPase. DR InterPro; IPR003439; ABC_transporter. DR Pfam; PF00005; ABC_tran; 2. DR ProDom; PD000006; ABC_transporter; 2. DR SMART; SM00382; AAA; 2. DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1. DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2. KW ATP-binding; Complete proteome; Repeat. FT DOMAIN 199 464 ABC transporter 1. FT DOMAIN 532 748 ABC transporter 2. FT NP_BIND 232 239 ATP (Potential). FT NP_BIND 565 572 ATP (Potential). SQ SEQUENCE 752 AA; 85027 MW; 486FED10305A572E CRC64; MASIGSQVRK AASSIDPIVT DYAVGYFNHL SGITFDAVQS KQVDLSTEVQ FVSDLLIDAG ASKAKVKELS ESILKQLTTQ LKENEAKLEL TGDTSKRLLD INVLKSHNSK SDINVSLSML GVNGDIEHTG RKMETRVDLK KLAKAEQKIA KKVAKRNNKF VKYEASKLIN DQKEEDYDSF FLQINPLEFG SSAGKSKDIH IDTFDLYVGD GQRILSNAQL TLSFGHRYGL VGQNGIGKST LLRALSRREL NVPKHVSILH VEQELRGDDT KALQSVLDAD VWRKQLLSEE AKINERLKEM DVLRQEFEED SLEVKKLDNE REDLDNHLIQ ISDKLVDMES DKAEARAASI LYGLGFSTEA QQQPTNSFSG GWRMRLSLAR ALFCQPDLLL LDEPSNMLDV PSIAYLAEYL KTYPNTVLTV SHDRAFLNEV ATDIIYQHNE RLDYYRGQDF DTFYTTKEER RKNAQREYDN QMVYRKHLQE FIDKYRYNAA KSQEAQSRIK KLEKLPVLEP PEQDKTIDFK FPECDKLSPP IIQLQDVSFG YDENNLLLKD VNLDVQMDSR IALVGANGCG KTTLLKIMME QLRPLKGFVS RNPRLRIGYF TQHHVDSMDL TTSAVDWMSK SFPGKTDEEY RRHLGSFGIT GTLGLQKMQL LSGGQKSRVA FAALCLNNPH ILVLDEPSNH LDTTGLDALV EALKNFNGGV LMVSHDISVI DSVCKEIWVS EQGTVKRFEG TIYDYRDYIL QSADAAGVVK KH //