ID S26A3_HUMAN Reviewed; 764 AA. AC P40879; DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1995, sequence version 1. DT 20-APR-2010, entry version 95. DE RecName: Full=Chloride anion exchanger; DE AltName: Full=Down-regulated in adenoma; DE Short=Protein DRA; DE AltName: Full=Solute carrier family 26 member 3; GN Name=SLC26A3; Synonyms=DRA; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Colon; RX MEDLINE=93248250; PubMed=7683425; DOI=10.1073/pnas.90.9.4166; RA Schweinfest C.W., Henderson K.W., Suster S., Kondoh N., Papas T.S.; RT "Identification of a colon mucosa gene that is down-regulated in colon RT adenomas and adenocarcinomas."; RL Proc. Natl. Acad. Sci. U.S.A. 90:4166-4170(1993). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Lung; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [3] RP SIMILARITY TO SULFATE PERMEASES. RX MEDLINE=94188926; PubMed=8140616; DOI=10.1016/0968-0004(94)90168-6; RA Sandal N.N., Marcker K.A.; RT "Similarities between a soybean nodulin, Neurospora crassa sulphate RT permease II and a putative human tumour suppressor."; RL Trends Biochem. Sci. 19:19-19(1994). RN [4] RP INTERACTION WITH PDZK1. RX PubMed=15766278; DOI=10.1021/bi048828b; RA Rossmann H., Jacob P., Baisch S., Hassoun R., Meier J., Natour D., RA Yahya K., Yun C., Biber J., Lackner K.J., Fiehn W., Gregor M., RA Seidler U., Lamprecht G.; RT "The CFTR associated protein CAP70 interacts with the apical Cl-/HCO3- RT exchanger DRA in rabbit small intestinal mucosa."; RL Biochemistry 44:4477-4487(2005). RN [5] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-503; SER-509 AND RP TYR-520, AND MASS SPECTROMETRY. RX PubMed=17525332; DOI=10.1126/science.1140321; RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, RA Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., RA Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; RT "ATM and ATR substrate analysis reveals extensive protein networks RT responsive to DNA damage."; RL Science 316:1160-1166(2007). RN [6] RP VARIANTS CLD LEU-124 AND VAL-317 DEL, AND VARIANT TRP-307. RX MEDLINE=97051927; PubMed=8896562; DOI=10.1038/ng1196-316; RA Hoeglund P., Haila S., Socha J., Tomaszewski L., Saarialho-Kere U., RA Karjalainen-Lindsberg M.-L., Airola K., Holmberg C., RA de la Chapelle A., Kere J.; RT "Mutations of the Down-regulated in adenoma (DRA) gene cause RT congenital chloride diarrhoea."; RL Nat. Genet. 14:316-319(1996). RN [7] RP VARIANTS CLD SER-120; ARG-131; VAL-317 DEL AND TYR-527 DEL. RX MEDLINE=98213471; PubMed=9554749; RX DOI=10.1002/(SICI)1098-1004(1998)11:4<321::AID-HUMU10>3.3.CO;2-1; RA Hoeglund P., Haila S., Gustavson K.-H., Taipale M., Hannula K., RA Popinska K., Holmberg C., Socha J., de la Chapelle A., Kere J.; RT "Clustering of private mutations in the congenital chloride RT diarrhea/down-regulated in adenoma gene."; RL Hum. Mutat. 11:321-327(1998). RN [8] RP VARIANTS CLD PRO-206 AND VAL-468. RX MEDLINE=21415581; PubMed=11524734; DOI=10.1002/humu.1179; RA Hoeglund P., Sormaala M., Haila S., Socha J., Rajaram U., RA Scheurlen W., Sinaasappel M., de Jonge H., Holmberg C., Yoshikawa H., RA Kere J.; RT "Identification of seven novel mutations including the first two RT genomic rearrangements in SLC26A3 mutated in congenital chloride RT diarrhea."; RL Hum. Mutat. 18:233-242(2001). CC -!- FUNCTION: Chloride/bicarbonate exchanger. Involved in absorbtion CC of in the colon. Helps mediate electrolyte and fluid absorption. CC -!- SUBUNIT: Interacts with PDZK1. CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein CC (Probable). CC -!- DEVELOPMENTAL STAGE: Expression is significantly decreased in CC adenomas (polyps) and adenocarcinomas of the colon. CC -!- PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. CC -!- DISEASE: Defects in SLC26A3 are the cause of congenital chloride CC diarrhea (CLD) [MIM:214700]. CLD is a disease characterized by CC voluminous watery stools containing an excess of chloride. The CC children with this disease are often premature. CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) CC family. CC -!- SIMILARITY: Contains 1 STAS domain. CC -!- WEB RESOURCE: Name=GeneReviews; CC URL="http://www.ncbi.nlm.nih.gov/sites/GeneTests/lab/gene/SLC26A3"; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L02785; AAA58443.1; -; mRNA. DR EMBL; BC025671; AAH25671.1; -; mRNA. DR IPI; IPI00031036; -. DR PIR; A47456; A47456. DR RefSeq; NP_000102.1; -. DR UniGene; Hs.1650; -. DR MINT; MINT-1787198; -. DR STRING; P40879; -. DR PhosphoSite; P40879; -. DR PRIDE; P40879; -. DR Ensembl; ENST00000340010; ENSP00000345873; ENSG00000091138; Homo sapiens. DR GeneID; 1811; -. DR KEGG; hsa:1811; -. DR NMPDR; fig|9606.3.peg.29280; -. DR UCSC; uc003ver.2; human. DR CTD; 1811; -. DR GeneCards; GC07M107193; -. DR H-InvDB; HIX0006993; -. DR HGNC; HGNC:3018; SLC26A3. DR MIM; 126650; gene. DR MIM; 214700; phenotype. DR Orphanet; 53689; Chloride diarrhea, congenital. DR PharmGKB; PA35044; -. DR eggNOG; prNOG05305; -. DR HOGENOM; HBG446299; -. DR HOVERGEN; HBG000639; -. DR InParanoid; P40879; -. DR OMA; QLKFMLQ; -. DR OrthoDB; EOG947JCV; -. DR PhylomeDB; P40879; -. DR Reactome; REACT_15518; Transmembrane transport of small molecules. DR NextBio; 7381; -. DR ArrayExpress; P40879; -. DR Bgee; P40879; -. DR CleanEx; HS_SLC26A3; -. DR Genevestigator; P40879; -. DR GermOnline; ENSG00000091138; Homo sapiens. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. DR GO; GO:0005624; C:membrane fraction; TAS:ProtInc. DR GO; GO:0008271; F:secondary active sulfate transmembrane tran...; IEA:InterPro. DR GO; GO:0003712; F:transcription cofactor activity; TAS:ProtInc. DR GO; GO:0003700; F:transcription factor activity; TAS:ProtInc. DR GO; GO:0007588; P:excretion; TAS:ProtInc. DR GO; GO:0008272; P:sulfate transport; IEA:InterPro. DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro. DR InterPro; IPR018045; S04_transporter_CS. DR InterPro; IPR002645; SO4_transptr/STAS. DR InterPro; IPR001902; SulP_transpt. DR InterPro; IPR011547; Sulph_transpt. DR Pfam; PF01740; STAS; 1. DR Pfam; PF00916; Sulfate_transp; 1. DR SUPFAM; SSF52091; STAS; 1. DR TIGRFAMs; TIGR00815; sulP; 1. DR PROSITE; PS01130; SLC26A; 1. DR PROSITE; PS50801; STAS; 1. PE 1: Evidence at protein level; KW Antiport; Chloride; Complete proteome; Disease mutation; Membrane; KW Phosphoprotein; Polymorphism; Transmembrane; Transport. FT CHAIN 1 764 Chloride anion exchanger. FT /FTId=PRO_0000080161. FT TRANSMEM 77 97 Potential. FT TRANSMEM 100 120 Potential. FT TRANSMEM 125 145 Potential. FT TRANSMEM 176 196 Potential. FT TRANSMEM 198 218 Potential. FT TRANSMEM 259 279 Potential. FT TRANSMEM 286 306 Potential. FT TRANSMEM 343 363 Potential. FT TRANSMEM 375 395 Potential. FT TRANSMEM 412 432 Potential. FT TRANSMEM 439 459 Potential. FT TRANSMEM 470 490 Potential. FT TRANSMEM 644 664 Potential. FT TRANSMEM 702 722 Potential. FT DOMAIN 525 720 STAS. FT MOD_RES 503 503 Phosphothreonine. FT MOD_RES 509 509 Phosphoserine. FT MOD_RES 520 520 Phosphotyrosine. FT VARIANT 68 68 R -> Q (in dbSNP:rs10280704). FT /FTId=VAR_053660. FT VARIANT 120 120 G -> S (in CLD). FT /FTId=VAR_007428. FT VARIANT 124 124 H -> L (in CLD). FT /FTId=VAR_007429. FT VARIANT 131 131 P -> R (in CLD). FT /FTId=VAR_007430. FT VARIANT 206 206 S -> P (in CLD). FT /FTId=VAR_012777. FT VARIANT 307 307 C -> W (in dbSNP:rs34407351). FT /FTId=VAR_007431. FT VARIANT 317 317 Missing (in CLD). FT /FTId=VAR_007432. FT VARIANT 468 468 D -> V (in CLD). FT /FTId=VAR_012778. FT VARIANT 527 527 Missing (in CLD). FT /FTId=VAR_007433. FT VARIANT 554 554 R -> Q (in dbSNP:rs2301635). FT /FTId=VAR_053661. FT VARIANT 753 753 N -> S (in dbSNP:rs35342296). FT /FTId=VAR_053662. SQ SEQUENCE 764 AA; 84505 MW; 694C5BC2D4121F6D CRC64; MIEPFGNQYI VARPVYSTNA FEENHKKTGR HHKTFLDHLK VCCSCSPQKA KRIVLSLFPI ASWLPAYRLK EWLLSDIVSG ISTGIVAVLQ GLAFALLVDI PPVYGLYASF FPAIIYLFFG TSRHISVGPF PILSMMVGLA VSGAVSKAVP DRNATTLGLP NNSNNSSLLD DERVRVAAAA SVTVLSGIIQ LAFGILRIGF VVIYLSESLI SGFTTAAAVH VLVSQLKFIF QLTVPSHTDP VSIFKVLYSV FSQIEKTNIA DLVTALIVLL VVSIVKEINQ RFKDKLPVPI PIEFIMTVIA AGVSYGCDFK NRFKVAVVGD MNPGFQPPIT PDVETFQNTV GDCFGIAMVA FAVAFSVASV YSLKYDYPLD GNQELIALGL GNIVCGVFRG FAGSTALSRS AVQESTGGKT QIAGLIGAII VLIVVLAIGF LLAPLQKSVL AALALGNLKG MLMQFAEIGR LWRKDKYDCL IWIMTFIFTI VLGLGLGLAA SVAFQLLTIV FRTQFPKCST LANIGRTNIY KNKKDYYDMY EPEGVKIFRC PSPIYFANIG FFRRKLIDAV GFSPLRILRK RNKALRKIRK LQKQGLLQVT PKGFICTVDT IKDSDEELDN NQIEVLDQPI NTTDLPFHID WNDDLPLNIE VPKISLHSLI LDFSAVSFLD VSSVRGLKSI LQEFIRIKVD VYIVGTDDDF IEKLNRYEFF DGEVKSSIFF LTIHDAVLHI LMKKDYSTSK FNPSQEKDGK IDFTINTNGG LRNRVYEVPV ETKF //