ID TEM1_YEAST STANDARD; PRT; 245 AA. AC P38987; DT 01-FEB-1995 (Rel. 31, Created) DT 01-FEB-1995 (Rel. 31, Last sequence update) DT 25-OCT-2004 (Rel. 45, Last annotation update) DE TEM1 protein. GN Name=TEM1; OrderedLocusNames=YML064C; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP SEQUENCE FROM N.A. RX MEDLINE=95021277; PubMed=7935462; RA Shirayama M., Matsui Y., Toh-E A.; RT "The yeast TEM1 gene, which encodes a GTP-binding protein, is involved RT in termination of M phase."; RL Mol. Cell. Biol. 14:7476-7482(1994). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=S288c / AB972; RX MEDLINE=97313268; PubMed=9169872; RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., RA Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., RA Rice P., Skelton J., Walsh S., Whitehead S., Barrell B.G.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome RT XIII."; RL Nature 387:90-93(1997). RN [3] RP SEQUENCE FROM N.A. RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., RA LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces RT cerevisiae ORFs in the Gateway recombinational cloning system."; RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: GTP-binding protein involved in termination of M phase. CC May play a role in triggering the degradation of G2 cyclin to CC inactivate M-phase promoting factor at the termination of mitosis. CC Acts upstream of CDC15 kinase and may be required to activate the CC CDC15 protein kinase pathway. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D38172; BAA07371.1; -. DR EMBL; Z38114; CAA86257.1; -. DR EMBL; AY557980; AAS56306.1; -. DR PIR; S48334; S48334. DR IntAct; P38987; -. DR GermOnline; 142595; -. DR SGD; S0004529; TEM1. DR GO; GO:0005515; F:protein binding; IPI. DR GO; GO:0000087; P:M phase of mitotic cell cycle; IMP. DR GO; GO:0007165; P:signal transduction; NAS. DR InterPro; IPR001806; Ras_trnsfrmng. DR InterPro; IPR005225; Small_GTP. DR Pfam; PF00071; Ras; 1. DR PRINTS; PR00449; RASTRNSFRMNG. DR TIGRFAMs; TIGR00231; small_GTP; 1. KW GTP-binding; Mitosis. FT NP_BIND 27 34 GTP (By similarity). FT NP_BIND 75 79 GTP (By similarity). FT NP_BIND 132 135 GTP (By similarity). SQ SEQUENCE 245 AA; 27296 MW; F650C8946A03707C CRC64; MATPSTGANN SIPAVRNQVE VQVGLVGDAQ VGKTSLMVKY VQNIYDKEYT QTLGVNFLKR KVSIRSTDII FSIMDLGGQR EFINMLPIAT VGSSVIIFLF DLTRPETLSS IKEWYRQAYG LNDSAIPILV GTKYDLLIDL DPEYQEQISR TSMKYAQVMN APLIFCSTAK SINIQKIFKI ALAKIFNLTL TIPEINEIGD PLLIYKHLGG QQHRHHNKSQ DRKSHNIRKP SSSPSSKAPS PGVNT //