ID ADY1_YEAST Reviewed; 237 AA. AC P38872; D3DLD3; DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1995, sequence version 1. DT 29-APR-2015, entry version 95. DE RecName: Full=Prospore formation at selected spindle poles protein 1; DE AltName: Full=Accumulates dyads protein 1; GN Name=PFS1; Synonyms=ADY1; OrderedLocusNames=YHR185C; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=8091229; DOI=10.1126/science.8091229; RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., RA Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., RA Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., RA Latreille P., Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., RA Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., RA Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., RA Vaudin M.; RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome RT VIII."; RL Science 265:2077-2082(1994). RN [2] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., RA Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and RT now."; RL G3 (Bethesda) 4:389-398(2014). RN [3] RP FUNCTION, AND SUBCELLULAR LOCATION. RX PubMed=11553705; DOI=10.1091/mbc.12.9.2646; RA Deng C., Saunders W.S.; RT "ADY1, a novel gene required for prospore membrane formation at RT selected spindle poles in Saccharomyces cerevisiae."; RL Mol. Biol. Cell 12:2646-2659(2001). CC -!- FUNCTION: Involved in the pathway that organizes the shaping and CC sizing of the prospore membrane (PSM) during sporulation. Required CC to localize MPC54 to all four spindle pole bodies, and localize CC DON1 and SPO14 to four prospore membranes. CC {ECO:0000269|PubMed:11553705}. CC -!- INTERACTION: CC P29295:HRR25; NbExp=3; IntAct=EBI-24859, EBI-8536; CC P40555:NAS2; NbExp=3; IntAct=EBI-24859, EBI-14024; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11553705}. CC Cytoplasm, cytoskeleton, microtubule organizing center, spindle CC pole body {ECO:0000269|PubMed:11553705}. Note=Nuclear in CC mononucleate meiotic cells. May not be localized to the spindle CC pole body or prospore membrane. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U00030; AAB68363.1; -; Genomic_DNA. DR EMBL; BK006934; DAA06877.1; -; Genomic_DNA. DR PIR; S46685; S46685. DR RefSeq; NP_012055.3; NM_001179316.3. DR ProteinModelPortal; P38872; -. DR BioGrid; 36618; 46. DR DIP; DIP-1372N; -. DR IntAct; P38872; 6. DR MINT; MINT-394703; -. DR STRING; 4932.YHR185C; -. DR EnsemblFungi; YHR185C; YHR185C; YHR185C. DR GeneID; 856591; -. DR KEGG; sce:YHR185C; -. DR CYGD; YHR185c; -. DR EuPathDB; FungiDB:YHR185C; -. DR SGD; S000001228; PFS1. DR eggNOG; NOG69411; -. DR InParanoid; P38872; -. DR OrthoDB; EOG780RZ1; -. DR BioCyc; YEAST:G3O-31215-MONOMER; -. DR NextBio; 982470; -. DR PRO; PR:P38872; -. DR Proteomes; UP000002311; Chromosome VIII. DR Genevestigator; P38872; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell. DR GO; GO:0030476; P:ascospore wall assembly; IMP:SGD. PE 1: Evidence at protein level; KW Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; KW Reference proteome; Sporulation. FT CHAIN 1 237 Prospore formation at selected spindle FT poles protein 1. FT /FTId=PRO_0000064460. SQ SEQUENCE 237 AA; 27884 MW; 027F01058C0CFE6E CRC64; MNQGYTQLSA PELKETKTSK LNKMNNFRSS PIAEIINKIP PDCGKIQNTT FPEFNPALRR RQHEQWPAYE KPIRVTDSMS PQLSSINCLP NLYPHGTLPL PNPYLSYLNH IEKVNCQDVK FSNWSVLHNS NNGFEIPTYF SPRTTQNMPC SEKVESWLER LPIFVGFDGY LFTNCFDYEY MLDWEETEFT FEKTSCMETD YSKALTDTDI IYIQEKKIEA LIRNQYLKEY EFSQKDF //