ID SLT11_YEAST Reviewed; 364 AA. AC P38241; D6VQ64; P89497; DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1994, sequence version 1. DT 13-NOV-2013, entry version 97. DE RecName: Full=Pre-mRNA-splicing factor SLT11; DE AltName: Full=Extracellular mutant protein 2; DE AltName: Full=Synthetic lethality with U2 protein 11; GN Name=ECM2; Synonyms=SLT11; OrderedLocusNames=YBR065C; GN ORFNames=YBR0614; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7813418; RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., RA Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., RA Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., RA Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., RA Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., RA Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., RA Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., RA Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., RA Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., RA Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., RA Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., RA van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., RA Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., RA Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.; RT "Complete DNA sequence of yeast chromosome II."; RL EMBO J. 13:5795-5809(1994). RN [2] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases. RN [3] RP FUNCTION. RX PubMed=9528778; RA Xu D., Field D.J., Tang S.-J., Moris A., Bobechko B.P., Friesen J.D.; RT "Synthetic lethality of yeast slt mutations with U2 small nuclear RNA RT mutations suggests functional interactions between U2 and U5 snRNPs RT that are important for both steps of pre-mRNA splicing."; RL Mol. Cell. Biol. 18:2055-2066(1998). RN [4] RP FUNCTION, AND INTERACTION WITH SLU7. RX PubMed=11158289; DOI=10.1128/MCB.21.4.1011-1023.2001; RA Xu D., Friesen J.D.; RT "Splicing factor slt11p and its involvement in formation of U2/U6 RT helix II in activation of the yeast spliceosome."; RL Mol. Cell. Biol. 21:1011-1023(2001). RN [5] RP MASS SPECTROMETRY, AND IDENTIFICATION IN THE CWC COMPLEX. RX PubMed=11884590; DOI=10.1128/MCB.22.7.2011-2024.2002; RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.; RT "Proteomics analysis reveals stable multiprotein complexes in both RT fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel RT pre-mRNA splicing factors, and snRNAs."; RL Mol. Cell. Biol. 22:2011-2024(2002). RN [6] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [7] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). CC -!- FUNCTION: Involved in pre-mRNA splicing. Facilitates the CC cooperative formation of U2/U6 helix II in association with stem CC II in the spliceosome. Binds to RNA. CC -!- SUBUNIT: Belongs to the CWC complex (or CEF1-associated complex), CC a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and CC at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CC CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, CC ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, CC PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, CC SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with SLU7. CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- MISCELLANEOUS: Present with 4260 molecules/cell in log phase SD CC medium. CC -!- SIMILARITY: Belongs to the SLT11 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z35934; CAA85008.1; -; Genomic_DNA. DR EMBL; Z35935; CAA85009.1; -; Genomic_DNA. DR EMBL; BK006936; DAA07184.1; -; Genomic_DNA. DR PIR; S45925; S45925. DR RefSeq; NP_009621.3; NM_001178413.3. DR ProteinModelPortal; P38241; -. DR DIP; DIP-5359N; -. DR IntAct; P38241; 12. DR MINT; MINT-570568; -. DR STRING; 4932.YBR065C; -. DR PaxDb; P38241; -. DR EnsemblFungi; YBR065C; YBR065C; YBR065C. DR GeneID; 852357; -. DR KEGG; sce:YBR065C; -. DR CYGD; YBR065c; -. DR SGD; S000000269; ECM2. DR eggNOG; NOG277760; -. DR GeneTree; ENSGT00390000002792; -. DR HOGENOM; HOG000154338; -. DR KO; K12872; -. DR OMA; CKICTLP; -. DR OrthoDB; EOG7SFJ6P; -. DR BioCyc; YEAST:G3O-29034-MONOMER; -. DR NextBio; 971116; -. DR Genevestigator; P38241; -. DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IGI:SGD. PE 1: Evidence at protein level; KW Complete proteome; mRNA processing; mRNA splicing; Nucleus; KW Reference proteome; RNA-binding; Spliceosome. FT CHAIN 1 364 Pre-mRNA-splicing factor SLT11. FT /FTId=PRO_0000212432. SQ SEQUENCE 364 AA; 40925 MW; BF21E687817A42B4 CRC64; MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDHL ISLVNEENVM TEEAKNDMMK RFLSLKNVKL GGAQITSDPS EADNIVDKLK NILLRATSDG PSTPLIKNTT ALYKNEKGAN EVKNLEKYAS VDISHILKKL PLNESFLKNP STKSFFLYNI DASIPEWKIT DTVSQLLGIK KWKDGNSLSL IVNHKAKCGG LRFQSSELGE RFVSKISETL VTPKGLKRGV LLIDRFRIFI IPWSSGFSAA SFGTNTAENI KLSLSLNKLI QLELGLSFPT KSTDNAKNDK KKTSKKVHKD RSKKSKPRAN KLTI //