ID SLT11_YEAST Reviewed; 364 AA. AC P38241; P89497; DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1994, sequence version 1. DT 10-AUG-2010, entry version 70. DE RecName: Full=Pre-mRNA-splicing factor SLT11; DE AltName: Full=Synthetic lethality with U2 protein 11; DE AltName: Full=Extracellular mutant protein 2; GN Name=ECM2; Synonyms=SLT11; OrderedLocusNames=YBR065C; GN ORFNames=YBR0614; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomyceta; OC Saccharomycotina; Saccharomycetes; Saccharomycetales; OC Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=95112788; PubMed=7813418; RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., RA Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., RA Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., RA Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., RA Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., RA Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., RA Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., RA Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., RA Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., RA Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., RA Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., RA van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., RA Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., RA Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.; RT "Complete DNA sequence of yeast chromosome II."; RL EMBO J. 13:5795-5809(1994). RN [2] RP FUNCTION. RX PubMed=9528778; RA Xu D., Field D.J., Tang S.-J., Moris A., Bobechko B.P., Friesen J.D.; RT "Synthetic lethality of yeast slt mutations with U2 small nuclear RNA RT mutations suggests functional interactions between U2 and U5 snRNPs RT that are important for both steps of pre-mRNA splicing."; RL Mol. Cell. Biol. 18:2055-2066(1998). RN [3] RP FUNCTION, AND INTERACTION WITH SLU7. RX PubMed=11158289; DOI=10.1128/MCB.21.4.1011-1023.2001; RA Xu D., Friesen J.D.; RT "Splicing factor slt11p and its involvement in formation of U2/U6 RT helix II in activation of the yeast spliceosome."; RL Mol. Cell. Biol. 21:1011-1023(2001). RN [4] RP MASS SPECTROMETRY, AND IDENTIFICATION IN THE CWC COMPLEX. RX MEDLINE=21881936; PubMed=11884590; RX DOI=10.1128/MCB.22.7.2011-2024.2002; RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.; RT "Proteomics analysis reveals stable multiprotein complexes in both RT fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel RT pre-mRNA splicing factors, and snRNAs."; RL Mol. Cell. Biol. 22:2011-2024(2002). RN [5] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [6] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-352, AND MASS RP SPECTROMETRY. RX PubMed=17287358; DOI=10.1073/pnas.0607084104; RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.; RT "Analysis of phosphorylation sites on proteins from Saccharomyces RT cerevisiae by electron transfer dissociation (ETD) mass RT spectrometry."; RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-153, AND MASS RP SPECTROMETRY. RX PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200; RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; RT "A multidimensional chromatography technology for in-depth RT phosphoproteome analysis."; RL Mol. Cell. Proteomics 7:1389-1396(2008). CC -!- FUNCTION: Involved in pre-mRNA splicing. Facilitates the CC cooperative formation of U2/U6 helix II in association with stem CC II in the spliceosome. Binds to RNA. CC -!- SUBUNIT: Belongs to the CWC complex (or PRP19-associated complex), CC a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and CC at least BUD13, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CC CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, CC MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, CC SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, CC SYF1, SYF2, RSE1 and YJU2. Interacts with SLU7. CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- MISCELLANEOUS: Present with 4260 molecules/cell in log phase SD CC medium. CC -!- SIMILARITY: Belongs to the SLT11 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z35934; CAA85008.1; -; Genomic_DNA. DR EMBL; Z35935; CAA85009.1; -; Genomic_DNA. DR PIR; S45925; S45925. DR ProteinModelPortal; P38241; -. DR DIP; DIP-5359N; -. DR IntAct; P38241; 30. DR MINT; MINT-570568; -. DR STRING; P38241; -. DR EnsemblFungi; YBR065C; YBR065C; YBR065C. DR GenomeReviews; Y13134_GR; YBR065C. DR KEGG; sce:YBR065C; -. DR NMPDR; fig|4932.3.peg.318; -. DR CYGD; YBR065c; -. DR SGD; S000000269; ECM2. DR eggNOG; fuNOG09718; -. DR HOGENOM; HBG204140; -. DR OMA; KAIMADK; -. DR OrthoDB; EOG97DBZC; -. DR PhylomeDB; P38241; -. DR NextBio; 971116; -. DR ArrayExpress; P38241; -. DR Genevestigator; P38241; -. DR GermOnline; YBR065C; Saccharomyces cerevisiae. DR GO; GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD. DR GO; GO:0000398; P:nuclear mRNA splicing, via spliceosome; IGI:SGD. PE 1: Evidence at protein level; KW Complete proteome; mRNA processing; mRNA splicing; Nucleus; KW Phosphoprotein; RNA-binding; Spliceosome. FT CHAIN 1 364 Pre-mRNA-splicing factor SLT11. FT /FTId=PRO_0000212432. FT MOD_RES 153 153 Phosphothreonine. FT MOD_RES 352 352 Phosphoserine. SQ SEQUENCE 364 AA; 40925 MW; BF21E687817A42B4 CRC64; MNDEINEPPP NICEQCLGDE ANIRMTKIPQ GSECKICTLP FTLYHFKTSK RSNNIIKTLI CVRCATQRNI CQCCMLDSRW HIPIQLRDHL ISLVNEENVM TEEAKNDMMK RFLSLKNVKL GGAQITSDPS EADNIVDKLK NILLRATSDG PSTPLIKNTT ALYKNEKGAN EVKNLEKYAS VDISHILKKL PLNESFLKNP STKSFFLYNI DASIPEWKIT DTVSQLLGIK KWKDGNSLSL IVNHKAKCGG LRFQSSELGE RFVSKISETL VTPKGLKRGV LLIDRFRIFI IPWSSGFSAA SFGTNTAENI KLSLSLNKLI QLELGLSFPT KSTDNAKNDK KKTSKKVHKD RSKKSKPRAN KLTI //