ID FL2D_YEAST Reviewed; 366 AA. AC P38236; D6VQ56; DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1994, sequence version 1. DT 24-JUL-2024, entry version 151. DE RecName: Full=Protein MUM2; DE AltName: Full=Muddled meiosis protein 2; GN Name=MUM2; OrderedLocusNames=YBR057C; ORFNames=YBR0514; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7597852; DOI=10.1002/yea.320110511; RA Aljinovic G., Pohl T.M.; RT "Sequence and analysis of 24 kb on chromosome II of Saccharomyces RT cerevisiae."; RL Yeast 11:475-479(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x; RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., RA Mewes H.-W., Kleine K.; RT "Complete DNA sequence of yeast chromosome II."; RL EMBO J. 13:5795-5809(1994). RN [3] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). RN [4] RP DISRUPTION PHENOTYPE. RX PubMed=12586695; DOI=10.1093/genetics/163.1.47; RA Enyenihi A.H., Saunders W.S.; RT "Large-scale functional genomic analysis of sporulation and meiosis in RT Saccharomyces cerevisiae."; RL Genetics 163:47-54(2003). RN [5] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., RA O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [6] RP IDENTIFICATION IN THE MIS COMPLEX, AND FUNCTION. RX PubMed=22685417; DOI=10.1371/journal.pgen.1002732; RA Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.; RT "RNA methylation by the MIS complex regulates a cell fate decision in RT yeast."; RL PLoS Genet. 8:E1002732-E1002732(2012). RN [7] RP FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, AND SUBCELLULAR LOCATION. RX PubMed=24269006; DOI=10.1016/j.cell.2013.10.047; RA Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P., RA Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., Carr S.A., RA Lander E.S., Fink G.R., Regev A.; RT "High-resolution mapping reveals a conserved, widespread, dynamic mRNA RT methylation program in yeast meiosis."; RL Cell 155:1409-1421(2013). RN [8] RP FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, INTERACTION WITH VIR1, RP IDENTIFICATION BY MASS SPECTROMETRY, AND DISRUPTION PHENOTYPE. RX PubMed=36930734; DOI=10.1093/genetics/iyad043; RA Park Z.M., Belnap E., Remillard M., Rose M.D.; RT "Vir1p, the yeast homolog of virilizer, is required for mRNA m6A RT methylation and meiosis."; RL Genetics 224:1-16(2023). CC -!- FUNCTION: Component of the MIS complex, a complex that mediates N6- CC methyladenosine (m6A) methylation of meiotic mRNAs and is required for CC initiation of meiosis, progression through the meiotic divisions and CC sporulation. {ECO:0000269|PubMed:22685417, ECO:0000269|PubMed:24269006, CC ECO:0000269|PubMed:36930734}. CC -!- SUBUNIT: Component of the MIS (mRNA N6-methyladenosine (m6A) CC methylation) complex, at least composed of IME4, KAR4, MUM2, SLZ1, and CC VIR1 (PubMed:22685417, PubMed:24269006, PubMed:36930734). Interacts CC with VIR1 (PubMed:36930734). {ECO:0000269|PubMed:22685417, CC ECO:0000269|PubMed:24269006, ECO:0000269|PubMed:36930734}. CC -!- INTERACTION: CC P38236; P41833: IME4; NbExp=3; IntAct=EBI-11619, EBI-9210; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24269006}. Nucleus, CC nucleolus {ECO:0000269|PubMed:24269006}. Note=Localizes in the CC nucleolus during the period of m6A mRNA accumulation. CC -!- DISRUPTION PHENOTYPE: Mutant is sporulation defective and fails to CC perform premeiotic DNA synthesis (PubMed:12586695). Increases the rate CC of degradation of KAR4 protein (PubMed:36930734). Decreases the level CC of VIR1 protein (PubMed:36930734). {ECO:0000269|PubMed:12586695, CC ECO:0000269|PubMed:36930734}. CC -!- MISCELLANEOUS: Present with 3400 molecules/cell in log phase SD medium. CC {ECO:0000269|PubMed:14562106}. CC -!- SIMILARITY: Belongs to the fl(2)d family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z35926; CAA85000.1; -; Genomic_DNA. DR EMBL; Z46260; CAA86401.1; -; Genomic_DNA. DR EMBL; BK006936; DAA07176.1; -; Genomic_DNA. DR PIR; S45915; S45915. DR RefSeq; NP_009613.1; NM_001178405.1. DR AlphaFoldDB; P38236; -. DR SMR; P38236; -. DR BioGRID; 32760; 336. DR ComplexPortal; CPX-3211; MIS complex. DR DIP; DIP-1299N; -. DR IntAct; P38236; 18. DR MINT; P38236; -. DR STRING; 4932.YBR057C; -. DR GlyGen; P38236; 3 sites, 1 O-linked glycan (3 sites). DR iPTMnet; P38236; -. DR PaxDb; 4932-YBR057C; -. DR PeptideAtlas; P38236; -. DR EnsemblFungi; YBR057C_mRNA; YBR057C; YBR057C. DR GeneID; 852348; -. DR KEGG; sce:YBR057C; -. DR AGR; SGD:S000000261; -. DR SGD; S000000261; MUM2. DR VEuPathDB; FungiDB:YBR057C; -. DR eggNOG; ENOG502S20W; Eukaryota. DR HOGENOM; CLU_067405_1_0_1; -. DR InParanoid; P38236; -. DR OMA; NANIWRD; -. DR OrthoDB; 1987562at2759; -. DR BioCyc; YEAST:G3O-29028-MONOMER; -. DR BioGRID-ORCS; 852348; 0 hits in 10 CRISPR screens. DR PRO; PR:P38236; -. DR Proteomes; UP000002311; Chromosome II. DR RNAct; P38236; Protein. DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB. DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB. DR GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IDA:UniProtKB. DR GO; GO:0051321; P:meiotic cell cycle; IMP:SGD. DR GO; GO:2000221; P:negative regulation of pseudohyphal growth; IMP:SGD. DR GO; GO:0006279; P:premeiotic DNA replication; IMP:SGD. DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. PE 1: Evidence at protein level; KW Cytoplasm; Meiosis; Nucleus; Reference proteome; Sporulation. FT CHAIN 1..366 FT /note="Protein MUM2" FT /id="PRO_0000096647" FT REGION 28..47 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 60..85 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 366 AA; 41433 MW; E57C853BFE8E5E83 CRC64; MNYMAYDYDP QHSLETSFNN LAFHPHQQSQ QQALYESGER NDARPGLMNT LGQASKMNNS MLPQGSSASP LTGQHSLNST TNFNMPPSMN TYNYQNVPQA SMRNTLNHNN IMNGATANDY WLDPMNNMTN NKDTNGNPND SMSSMSNMTA KTSINSTAFK NSFVPFNHVT ALSMNNVNSN EMNSNKDDRM EALEVELQIK ESQIESLENE IQRLKKIFNE GLNYKQNEHK YEKENCHIPQ TFELPASLEV IFRKLSSSLH AKEKELAETK ENLESILTAL ALNPTNSVTK YGRYDAESIA HKMVVRLENL TNENKEMAKM LAYGRSKETQ IELQLAKKEN LELREKIASL EAHLASKESS KEDVAN //