ID MUM2_YEAST Reviewed; 366 AA. AC P38236; D6VQ56; DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1994, sequence version 1. DT 07-SEP-2016, entry version 106. DE RecName: Full=Protein MUM2; DE AltName: Full=Muddled meiosis protein 2; GN Name=MUM2; OrderedLocusNames=YBR057C; ORFNames=YBR0514; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7597852; DOI=10.1002/yea.320110511; RA Aljinovic G., Pohl T.M.; RT "Sequence and analysis of 24 kb on chromosome II of Saccharomyces RT cerevisiae."; RL Yeast 11:475-479(1995). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7813418; RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., RA Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., RA Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., RA Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., RA Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., RA Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., RA Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., RA Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., RA Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., RA Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., RA Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., RA van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., RA Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., RA Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.; RT "Complete DNA sequence of yeast chromosome II."; RL EMBO J. 13:5795-5809(1994). RN [3] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., RA Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and RT now."; RL G3 (Bethesda) 4:389-398(2014). RN [4] RP DISRUPTION PHENOTYPE. RX PubMed=12586695; RA Enyenihi A.H., Saunders W.S.; RT "Large-scale functional genomic analysis of sporulation and meiosis in RT Saccharomyces cerevisiae."; RL Genetics 163:47-54(2003). RN [5] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [6] RP IDENTIFICATION IN THE MIS COMPLEX, AND FUNCTION. RX PubMed=22685417; DOI=10.1371/journal.pgen.1002732; RA Agarwala S.D., Blitzblau H.G., Hochwagen A., Fink G.R.; RT "RNA methylation by the MIS complex regulates a cell fate decision in RT yeast."; RL PLoS Genet. 8:E1002732-E1002732(2012). RN [7] RP FUNCTION, IDENTIFICATION IN THE MIS COMPLEX, AND SUBCELLULAR LOCATION. RX PubMed=24269006; DOI=10.1016/j.cell.2013.10.047; RA Schwartz S., Agarwala S.D., Mumbach M.R., Jovanovic M., Mertins P., RA Shishkin A., Tabach Y., Mikkelsen T.S., Satija R., Ruvkun G., RA Carr S.A., Lander E.S., Fink G.R., Regev A.; RT "High-resolution mapping reveals a conserved, widespread, dynamic mRNA RT methylation program in yeast meiosis."; RL Cell 155:1409-1421(2013). CC -!- FUNCTION: Component of the MIS complex, a complex that mediates CC N6-methyladenosine (m6A) methylation on some mRNAs during meiosis CC and is required for sporulation. {ECO:0000269|PubMed:22685417, CC ECO:0000269|PubMed:24269006}. CC -!- SUBUNIT: Component of the MIS complex, composed of SLZ1, MUM2 and CC IME4. {ECO:0000269|PubMed:22685417, ECO:0000269|PubMed:24269006}. CC -!- INTERACTION: CC P41833:IME4; NbExp=3; IntAct=EBI-11619, EBI-9210; CC P53185:YGL036W; NbExp=3; IntAct=EBI-11619, EBI-11537; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24269006}. CC Nucleus, nucleolus {ECO:0000269|PubMed:24269006}. Note=Localizes CC in the nucleolus during the period of m6A mRNA accumulation. CC -!- DISRUPTION PHENOTYPE: Mutant is sporulation defective and fails to CC perform premeiotic DNA synthesis. {ECO:0000269|PubMed:12586695}. CC -!- MISCELLANEOUS: Present with 3400 molecules/cell in log phase SD CC medium. {ECO:0000269|PubMed:14562106}. CC -!- SIMILARITY: Belongs to the fl(2)d family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z35926; CAA85000.1; -; Genomic_DNA. DR EMBL; Z46260; CAA86401.1; -; Genomic_DNA. DR EMBL; BK006936; DAA07176.1; -; Genomic_DNA. DR PIR; S45915; S45915. DR RefSeq; NP_009613.1; NM_001178405.1. DR ProteinModelPortal; P38236; -. DR SMR; P38236; 193-218. DR BioGrid; 32760; 185. DR DIP; DIP-1299N; -. DR IntAct; P38236; 16. DR MINT; MINT-399622; -. DR iPTMnet; P38236; -. DR MaxQB; P38236; -. DR EnsemblFungi; YBR057C; YBR057C; YBR057C. DR GeneID; 852348; -. DR KEGG; sce:YBR057C; -. DR EuPathDB; FungiDB:YBR057C; -. DR SGD; S000000261; MUM2. DR HOGENOM; HOG000000720; -. DR InParanoid; P38236; -. DR OMA; AYGRSKE; -. DR OrthoDB; EOG092C3ACL; -. DR BioCyc; YEAST:G3O-29028-MONOMER; -. DR PRO; PR:P38236; -. DR Proteomes; UP000002311; Chromosome II. DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB. DR GO; GO:0036396; C:MIS complex; IDA:UniProtKB. DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB. DR GO; GO:0007126; P:meiotic nuclear division; IMP:SGD. DR GO; GO:0080009; P:mRNA methylation; IMP:SGD. DR GO; GO:2000221; P:negative regulation of pseudohyphal growth; IMP:SGD. DR GO; GO:0006279; P:premeiotic DNA replication; IMP:SGD. DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW. PE 1: Evidence at protein level; KW Complete proteome; Cytoplasm; Meiosis; Nucleus; Reference proteome; KW Sporulation. FT CHAIN 1 366 Protein MUM2. FT /FTId=PRO_0000096647. SQ SEQUENCE 366 AA; 41433 MW; E57C853BFE8E5E83 CRC64; MNYMAYDYDP QHSLETSFNN LAFHPHQQSQ QQALYESGER NDARPGLMNT LGQASKMNNS MLPQGSSASP LTGQHSLNST TNFNMPPSMN TYNYQNVPQA SMRNTLNHNN IMNGATANDY WLDPMNNMTN NKDTNGNPND SMSSMSNMTA KTSINSTAFK NSFVPFNHVT ALSMNNVNSN EMNSNKDDRM EALEVELQIK ESQIESLENE IQRLKKIFNE GLNYKQNEHK YEKENCHIPQ TFELPASLEV IFRKLSSSLH AKEKELAETK ENLESILTAL ALNPTNSVTK YGRYDAESIA HKMVVRLENL TNENKEMAKM LAYGRSKETQ IELQLAKKEN LELREKIASL EAHLASKESS KEDVAN //