ID RM36_YEAST Reviewed; 177 AA. AC P36531; D6VQC0; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 20-DEC-2005, sequence version 3. DT 05-APR-2011, entry version 81. DE RecName: Full=54S ribosomal protein L36, mitochondrial; DE AltName: Full=YmL36; DE Flags: Precursor; GN Name=MRPL36; OrderedLocusNames=YBR122C; ORFNames=YBR0918; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=95208357; PubMed=7900426; DOI=10.1002/yea.320101014; RA Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.; RT "Analysis of a 70 kb region on the right arm of yeast chromosome II."; RL Yeast 10:1363-1381(1994). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=95112788; PubMed=7813418; RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., RA Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., RA Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., RA Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., RA Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., RA Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., RA Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., RA Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., RA Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., RA Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., RA Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., RA van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., RA Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., RA Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.; RT "Complete DNA sequence of yeast chromosome II."; RL EMBO J. 13:5795-5809(1994). RN [3] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases. RN [4] RP PROTEIN SEQUENCE OF 15-51, AND SUBUNIT. RC STRAIN=07173; RX MEDLINE=91285106; PubMed=2060626; DOI=10.1016/0014-5793(91)80759-V; RA Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., RA Kitakawa M.; RT "Extended N-terminal sequencing of proteins of the large ribosomal RT subunit from yeast mitochondria."; RL FEBS Lett. 284:51-56(1991). RN [5] RP FUNCTION. RX PubMed=11259585; DOI=10.1128/MCB.21.7.2359-2372.2001; RA Bonnefoy N., Bsat N., Fox T.D.; RT "Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is RT controlled by the nucleotide sequence specifying the pre-Cox2p leader RT peptide."; RL Mol. Cell. Biol. 21:2359-2372(2001). RN [6] RP IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, AND MASS RP SPECTROMETRY. RX MEDLINE=22280971; PubMed=12392552; RX DOI=10.1046/j.1432-1033.2002.03226.x; RA Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.; RT "Tag-mediated isolation of yeast mitochondrial ribosome and mass RT spectrometric identification of its new components."; RL Eur. J. Biochem. 269:5203-5214(2002). RN [7] RP IDENTIFICATION OF PROBABLE INITIATION SITE. RX MEDLINE=22633889; PubMed=12748633; DOI=10.1038/nature01644; RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.; RT "Sequencing and comparison of yeast species to identify genes and RT regulatory elements."; RL Nature 423:241-254(2003). RN [8] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [9] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY. RC STRAIN=ATCC 76625 / YPH499; RX MEDLINE=22975177; PubMed=14576278; DOI=10.1073/pnas.2135385100; RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., RA Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., RA Rehling P., Pfanner N., Meisinger C.; RT "The proteome of Saccharomyces cerevisiae mitochondria."; RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003). RN [10] RP FUNCTION, AND DOMAIN. RX PubMed=15166137; DOI=10.1534/genetics.167.1.65; RA Williams E.H., Perez-Martinez X., Fox T.D.; RT "MrpL36p, a highly diverged L31 ribosomal protein homolog with RT additional functional domains in Saccharomyces cerevisiae RT mitochondria."; RL Genetics 167:65-75(2004). CC -!- FUNCTION: Involved in mitochondrial translation. Overexpression CC suppresses mutations in the COX2 leader peptide-encoding and CC initiation codon regions. CC -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit. CC Mature mitochondrial ribosomes consist of a small (37S) and a CC large (54S) subunit. The 37S subunit contains at least 33 CC different proteins and 1 molecule of RNA (15S). The 54S subunit CC contains at least 45 different proteins and 1 molecule of RNA CC (21S). CC -!- INTERACTION: CC P43603:LSB3; NbExp=1; IntAct=EBI-15582, EBI-22980; CC P32793:YSC84; NbExp=1; IntAct=EBI-15582, EBI-24460; CC -!- SUBCELLULAR LOCATION: Mitochondrion. CC -!- DOMAIN: Contains two functional domains. The central domain is CC sufficient for general mitochondrial translation but not CC suppression of COX2 mutants. The C-terminus sequence is sufficient CC for dosage suppression of COX2 mutants. CC -!- SIMILARITY: Belongs to the ribosomal protein L31P family. Highly CC divergent. CC -!- SEQUENCE CAUTION: CC Sequence=CAA55624.1; Type=Erroneous initiation; CC Sequence=CAA85079.1; Type=Erroneous initiation; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X78993; CAA55624.1; ALT_INIT; Genomic_DNA. DR EMBL; Z35991; CAA85079.1; ALT_INIT; Genomic_DNA. DR EMBL; BK006936; DAA07240.1; -; Genomic_DNA. DR PIR; S44701; S44701. DR RefSeq; NP_009680.2; NM_001178470.1. DR ProteinModelPortal; P36531; -. DR SMR; P36531; 46-108. DR DIP; DIP-4628N; -. DR IntAct; P36531; 17. DR MINT; MINT-566842; -. DR STRING; P36531; -. DR PeptideAtlas; P36531; -. DR PRIDE; P36531; -. DR EnsemblFungi; YBR122C; YBR122C; YBR122C. DR GeneID; 852419; -. DR NMPDR; fig|4932.3.peg.382; -. DR CYGD; YBR122c; -. DR SGD; S000000326; MRPL36. DR eggNOG; fuNOG10049; -. DR HOGENOM; HBG397282; -. DR OMA; RPAIYHQ; -. DR OrthoDB; EOG45F103; -. DR PhylomeDB; P36531; -. DR NextBio; 971282; -. DR ArrayExpress; P36531; -. DR Genevestigator; P36531; -. DR GermOnline; YBR122C; Saccharomyces cerevisiae. DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; IPI:SGD. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0003735; F:structural constituent of ribosome; IPI:SGD. DR GO; GO:0032543; P:mitochondrial translation; IC:SGD. PE 1: Evidence at protein level; KW Complete proteome; Direct protein sequencing; Mitochondrion; KW Ribonucleoprotein; Ribosomal protein; Transit peptide. FT TRANSIT 1 14 Mitochondrion. FT CHAIN 15 177 54S ribosomal protein L36, mitochondrial. FT /FTId=PRO_0000030580. FT REGION 36 118 Sufficient for general mitochondrial FT translation. FT REGION 87 177 Sufficient for dosage suppression of COX2 FT mutation. SQ SEQUENCE 177 AA; 20091 MW; 7A8DF86EE899B848 CRC64; MLKSIFAKRF ASTGSYPGST RITLPRRPAK KIQLGKSRPA IYHQFNVKME LSDGSVVIRR SQYPKGEIRL IQDQRNNPLW NPSRDDLVVV DANSGGSLDR FNKRYSSLFS VDSTTPNSSS ETVELSEENK KKTQIKKEEK EDVSEKAFGM DDYLSLLDDS EQQIKSGKLA SKKRDKK //