ID RM36_YEAST Reviewed; 177 AA. AC P36531; D6VQC0; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 20-DEC-2005, sequence version 3. DT 16-OCT-2019, entry version 146. DE RecName: Full=54S ribosomal protein L36, mitochondrial; DE AltName: Full=Mitochondrial large ribosomal subunit protein bL31m {ECO:0000303|PubMed:24675956}; DE AltName: Full=YmL36; DE Flags: Precursor; GN Name=MRPL36; OrderedLocusNames=YBR122C; ORFNames=YBR0918; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7900426; DOI=10.1002/yea.320101014; RA Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.; RT "Analysis of a 70 kb region on the right arm of yeast chromosome II."; RL Yeast 10:1363-1381(1994). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x; RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., RA Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., RA Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., RA Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., RA Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., RA Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., RA Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., RA Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., RA Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., RA Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., RA Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., RA van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., RA Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., RA Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.; RT "Complete DNA sequence of yeast chromosome II."; RL EMBO J. 13:5795-5809(1994). RN [3] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., RA Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and RT now."; RL G3 (Bethesda) 4:389-398(2014). RN [4] RP PROTEIN SEQUENCE OF 15-51, AND SUBUNIT. RC STRAIN=07173; RX PubMed=2060626; DOI=10.1016/0014-5793(91)80759-v; RA Grohmann L., Graack H.-R., Kruft V., Choli T., Goldschmidt-Reisin S., RA Kitakawa M.; RT "Extended N-terminal sequencing of proteins of the large ribosomal RT subunit from yeast mitochondria."; RL FEBS Lett. 284:51-56(1991). RN [5] RP FUNCTION. RX PubMed=11259585; DOI=10.1128/mcb.21.7.2359-2372.2001; RA Bonnefoy N., Bsat N., Fox T.D.; RT "Mitochondrial translation of Saccharomyces cerevisiae COX2 mRNA is RT controlled by the nucleotide sequence specifying the pre-Cox2p leader RT peptide."; RL Mol. Cell. Biol. 21:2359-2372(2001). RN [6] RP IDENTIFICATION IN THE MITOCHONDRIAL RIBOSOMAL LARGE COMPLEX, AND RP IDENTIFICATION BY MASS SPECTROMETRY. RX PubMed=12392552; DOI=10.1046/j.1432-1033.2002.03226.x; RA Gan X., Kitakawa M., Yoshino K., Oshiro N., Yonezawa K., Isono K.; RT "Tag-mediated isolation of yeast mitochondrial ribosome and mass RT spectrometric identification of its new components."; RL Eur. J. Biochem. 269:5203-5214(2002). RN [7] RP IDENTIFICATION OF PROBABLE INITIATION SITE. RX PubMed=12748633; DOI=10.1038/nature01644; RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.; RT "Sequencing and comparison of yeast species to identify genes and RT regulatory elements."; RL Nature 423:241-254(2003). RN [8] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [9] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RC STRAIN=ATCC 76625 / YPH499; RX PubMed=14576278; DOI=10.1073/pnas.2135385100; RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., RA Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., RA Rehling P., Pfanner N., Meisinger C.; RT "The proteome of Saccharomyces cerevisiae mitochondria."; RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003). RN [10] RP FUNCTION, AND DOMAIN. RX PubMed=15166137; DOI=10.1534/genetics.167.1.65; RA Williams E.H., Perez-Martinez X., Fox T.D.; RT "MrpL36p, a highly diverged L31 ribosomal protein homolog with RT additional functional domains in Saccharomyces cerevisiae RT mitochondria."; RL Genetics 167:65-75(2004). RN [11] RP SUBCELLULAR LOCATION. RX PubMed=25609543; DOI=10.1038/ncomms7019; RA Pfeffer S., Woellhaf M.W., Herrmann J.M., Forster F.; RT "Organization of the mitochondrial translation machinery studied in RT situ by cryoelectron tomography."; RL Nat. Commun. 6:6019-6019(2015). RN [12] RP STRUCTURE BY ELECTRON MICROSCOPY (3.20 ANGSTROMS), AND SUBUNIT. RX PubMed=24675956; DOI=10.1126/science.1249410; RA Amunts A., Brown A., Bai X.C., Llacer J.L., Hussain T., Emsley P., RA Long F., Murshudov G., Scheres S.H., Ramakrishnan V.; RT "Structure of the yeast mitochondrial large ribosomal subunit."; RL Science 343:1485-1489(2014). CC -!- FUNCTION: Component of the mitochondrial ribosome (mitoribosome), CC a dedicated translation machinery responsible for the synthesis of CC mitochondrial genome-encoded proteins, including at least some of CC the essential transmembrane subunits of the mitochondrial CC respiratory chain. The mitoribosomes are attached to the CC mitochondrial inner membrane and translation products are CC cotranslationally integrated into the membrane (PubMed:25609543, CC PubMed:24675956). Overexpression of bL31m suppresses mutations in CC the COX2 leader peptide-encoding and initiation codon regions CC (PubMed:11259585, PubMed:15166137). {ECO:0000269|PubMed:11259585, CC ECO:0000269|PubMed:15166137, ECO:0000305|PubMed:24675956, CC ECO:0000305|PubMed:25609543}. CC -!- FUNCTION: Overexpression suppresses mutations in the COX2 leader CC peptide-encoding and initiation codon regions. CC {ECO:0000269|PubMed:11259585, ECO:0000269|PubMed:15166137}. CC -!- SUBUNIT: Component of the mitochondrial large ribosomal subunit CC (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a CC small (37S) and a large (54S) subunit. The 37S small subunit CC contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different CC proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) CC and 46 different proteins. {ECO:0000269|PubMed:12392552, CC ECO:0000269|PubMed:2060626, ECO:0000269|PubMed:24675956}. CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095, CC ECO:0000269|PubMed:14576278}. Note=Mitoribosomes are tethered to CC the mitochondrial inner membrane and spatially aligned with the CC membrane insertion machinery through two distinct membrane contact CC sites, formed by the 21S rRNA expansion segment 96-ES1 and the CC inner membrane protein MBA1. {ECO:0000269|PubMed:25609543}. CC -!- DOMAIN: Contains two functional domains. The central domain is CC sufficient for general mitochondrial translation but not CC suppression of COX2 mutants. The C-terminus sequence is sufficient CC for dosage suppression of COX2 mutants. CC {ECO:0000269|PubMed:15166137}. CC -!- SIMILARITY: Belongs to the bacterial ribosomal protein bL31 CC family. Highly divergent. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=CAA55624.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC Sequence=CAA85079.1; Type=Erroneous initiation; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X78993; CAA55624.1; ALT_INIT; Genomic_DNA. DR EMBL; Z35991; CAA85079.1; ALT_INIT; Genomic_DNA. DR EMBL; BK006936; DAA07240.1; -; Genomic_DNA. DR PIR; S44701; S44701. DR RefSeq; NP_009680.2; NM_001178470.1. DR PDB; 3J6B; EM; 3.20 A; V=1-177. DR PDB; 5MRC; EM; 3.25 A; V=1-177. DR PDB; 5MRE; EM; 3.75 A; V=1-177. DR PDB; 5MRF; EM; 4.97 A; V=1-177. DR PDBsum; 3J6B; -. DR PDBsum; 5MRC; -. DR PDBsum; 5MRE; -. DR PDBsum; 5MRF; -. DR SMR; P36531; -. DR BioGrid; 32824; 131. DR ComplexPortal; CPX-1602; 54S mitochondrial large ribosomal subunit. DR DIP; DIP-4628N; -. DR IntAct; P36531; 14. DR MINT; P36531; -. DR STRING; 4932.YBR122C; -. DR MaxQB; P36531; -. DR PaxDb; P36531; -. DR PRIDE; P36531; -. DR EnsemblFungi; YBR122C_mRNA; YBR122C; YBR122C. DR GeneID; 852419; -. DR KEGG; sce:YBR122C; -. DR EuPathDB; FungiDB:YBR122C; -. DR SGD; S000000326; MRPL36. DR HOGENOM; HOG000248746; -. DR InParanoid; P36531; -. DR OMA; GRSWDAN; -. DR BioCyc; YEAST:G3O-29079-MONOMER; -. DR PRO; PR:P36531; -. DR Proteomes; UP000002311; Chromosome II. DR GO; GO:0005762; C:mitochondrial large ribosomal subunit; IDA:SGD. DR GO; GO:0005739; C:mitochondrion; HDA:SGD. DR GO; GO:0003735; F:structural constituent of ribosome; IDA:SGD. DR GO; GO:0032543; P:mitochondrial translation; IMP:SGD. DR InterPro; IPR034600; MRPL36_yeast. DR PANTHER; PTHR28174; PTHR28174; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Direct protein sequencing; KW Mitochondrion; Reference proteome; Ribonucleoprotein; KW Ribosomal protein; Transit peptide. FT TRANSIT 1 14 Mitochondrion. FT {ECO:0000269|PubMed:2060626}. FT CHAIN 15 177 54S ribosomal protein L36, mitochondrial. FT /FTId=PRO_0000030580. FT REGION 36 118 Sufficient for general mitochondrial FT translation. FT REGION 87 177 Sufficient for dosage suppression of COX2 FT mutation. SQ SEQUENCE 177 AA; 20091 MW; 7A8DF86EE899B848 CRC64; MLKSIFAKRF ASTGSYPGST RITLPRRPAK KIQLGKSRPA IYHQFNVKME LSDGSVVIRR SQYPKGEIRL IQDQRNNPLW NPSRDDLVVV DANSGGSLDR FNKRYSSLFS VDSTTPNSSS ETVELSEENK KKTQIKKEEK EDVSEKAFGM DDYLSLLDDS EQQIKSGKLA SKKRDKK //