ID CUE2_YEAST Reviewed; 443 AA. AC P36075; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-1994, sequence version 1. DT 10-JUN-2008, entry version 61. DE Ubiquitin-binding protein CUE2 (Coupling of ubiquitin conjugation to DE ER degradation protein 2). GN Name=CUE2; OrderedLocusNames=YKL090W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 96604 / S288c / FY1679; RX MEDLINE=94255012; PubMed=8196765; DOI=10.1038/369371a0; RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., RA Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A., RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., RA Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., RA Zimmermann J., Haasemann M., Becker I., Mewes H.-W.; RT "Complete DNA sequence of yeast chromosome XI."; RL Nature 369:371-378(1994). RN [2] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411, AND MASS RP SPECTROMETRY. RX PubMed=15665377; DOI=10.1074/mcp.M400219-MCP200; RA Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., RA Mann M., Jensen O.N.; RT "Quantitative phosphoproteomics applied to the yeast pheromone RT signaling pathway."; RL Mol. Cell. Proteomics 4:310-327(2005). RN [3] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-112, AND RP MASS SPECTROMETRY. RX PubMed=17563356; DOI=10.1073/pnas.0701622104; RA Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.; RT "Proteome-wide identification of in vivo targets of DNA damage RT checkpoint kinases."; RL Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007). RN [4] RP STRUCTURE BY NMR OF 1-54 IN COMPLEX WITH UBIQUITIN. RX PubMed=12787503; DOI=10.1016/S0092-8674(03)00362-3; RA Kang R.S., Daniels C.M., Francis S.A., Shih S.C., Salerno W.J., RA Hicke L., Radhakrishnan I.; RT "Solution structure of a CUE-ubiquitin complex reveals a conserved RT mode of ubiquitin binding."; RL Cell 113:621-630(2003). CC -!- SUBUNIT: Binds to ubiquitin. CC -!- SIMILARITY: Contains 2 CUE domains. CC -!- SIMILARITY: Contains 1 Smr domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z28090; CAA81928.1; -; Genomic_DNA. DR PIR; S37915; S37915. DR RefSeq; NP_012833.1; -. DR PDB; 1OTR; NMR; -; A=6-54. DR PDBsum; 1OTR; -. DR DIP; DIP:1917N; -. DR IntAct; P36075; -. DR Ensembl; YKL090W; Saccharomyces cerevisiae. DR GeneID; 853772; -. DR GenomeReviews; Y13137_GR; YKL090W. DR KEGG; sce:YKL090W; -. DR NMPDR; fig|4932.3.peg.3817; -. DR CYGD; YKL090w; -. DR SGD; S000001573; CUE2. DR HOGENOM; P36075; -. DR LinkHub; P36075; -. DR ArrayExpress; P36075; -. DR GermOnline; YKL090W; Saccharomyces cerevisiae. DR GO; GO:0005515; F:protein binding; IDA:SGD. DR InterPro; IPR003892; CUE. DR InterPro; IPR002625; Smr/MutS2_C. DR Pfam; PF02845; CUE; 2. DR SMART; SM00546; CUE; 2. DR SMART; SM00463; SMR; 1. DR PROSITE; PS51140; CUE; 2. DR PROSITE; PS50828; SMR; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Phosphoprotein; Repeat; KW Ubl conjugation pathway. FT CHAIN 1 443 Ubiquitin-binding protein CUE2. FT /FTId=PRO_0000079558. FT DOMAIN 8 51 CUE 1. FT DOMAIN 55 98 CUE 2. FT DOMAIN 347 443 Smr. FT MOD_RES 109 109 Phosphoserine. FT MOD_RES 112 112 Phosphoserine. FT MOD_RES 411 411 Phosphoserine. FT HELIX 9 19 FT STRAND 21 23 FT HELIX 25 34 FT TURN 35 37 FT HELIX 40 49 SQ SEQUENCE 443 AA; 50874 MW; 8B7BB872C2BDC734 CRC64; MSMDNNDDHE SKLSILMDMF PAISKSKLQV HLLENNNDLD LTIGLLLKEN DDKSTVDNEL HQLYDMFPQL DCSVIKDQFV INEKSVESTI SDLLNYETLQ KLKDNQANSP DSVKRNEKKN NWESTNDHIE SIIKFTDAPK NIAQEYLAEN GFDTVKAIIK IILDYYDKRD FKKDVDTFKV KRSPNTTVRG GRVQSSTGLA HVLKKGKESA NVAQESLKRP RSYKHSLDSP QMVELNELVA DNRDLKAINH EFLQKCLQFY DGDVVKVLNI SSLLIEDDKN ITKTWNFDEG FTLTSRDNCK QHLPKFSTPQ ISRRNEVGNT YKLPLHDKET PEGAVPVINN LFQTYRLDFH GFLPSEAVST LKLALNKWWS KEVAERELNS HNINSYGSKV QFVSPLIVVT GRGIHSIGGI SKVRLQVKSF LEKNHYIFWE ESSYFRIEGK KKK //