ID CUE2_YEAST Reviewed; 443 AA. AC P36075; D6VXJ8; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-1994, sequence version 1. DT 16-SEP-2015, entry version 119. DE RecName: Full=Ubiquitin-binding protein CUE2; DE AltName: Full=Coupling of ubiquitin conjugation to ER degradation protein 2; GN Name=CUE2; OrderedLocusNames=YKL090W; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=8196765; DOI=10.1038/369371a0; RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., RA Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A., RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., RA Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., RA Zimmermann J., Haasemann M., Becker I., Mewes H.-W.; RT "Complete DNA sequence of yeast chromosome XI."; RL Nature 369:371-378(1994). RN [2] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., RA Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and RT now."; RL G3 (Bethesda) 4:389-398(2014). RN [3] RP STRUCTURE BY NMR OF 1-54 IN COMPLEX WITH UBIQUITIN. RX PubMed=12787503; DOI=10.1016/S0092-8674(03)00362-3; RA Kang R.S., Daniels C.M., Francis S.A., Shih S.C., Salerno W.J., RA Hicke L., Radhakrishnan I.; RT "Solution structure of a CUE-ubiquitin complex reveals a conserved RT mode of ubiquitin binding."; RL Cell 113:621-630(2003). CC -!- SUBUNIT: Binds to ubiquitin. {ECO:0000269|PubMed:12787503}. CC -!- SIMILARITY: Contains 2 CUE domains. {ECO:0000255|PROSITE- CC ProRule:PRU00468}. CC -!- SIMILARITY: Contains 1 Smr domain. {ECO:0000255|PROSITE- CC ProRule:PRU00321}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z28090; CAA81928.1; -; Genomic_DNA. DR EMBL; BK006944; DAA09068.1; -; Genomic_DNA. DR PIR; S37915; S37915. DR RefSeq; NP_012833.1; NM_001179656.1. DR PDB; 1OTR; NMR; -; A=6-54. DR PDBsum; 1OTR; -. DR ProteinModelPortal; P36075; -. DR SMR; P36075; 6-54. DR BioGrid; 34043; 11. DR DIP; DIP-1917N; -. DR IntAct; P36075; 4. DR MINT; MINT-394365; -. DR MaxQB; P36075; -. DR PaxDb; P36075; -. DR EnsemblFungi; YKL090W; YKL090W; YKL090W. DR GeneID; 853772; -. DR KEGG; sce:YKL090W; -. DR CYGD; YKL090w; -. DR EuPathDB; FungiDB:YKL090W; -. DR SGD; S000001573; CUE2. DR eggNOG; NOG262951; -. DR InParanoid; P36075; -. DR OMA; NDINLAC; -. DR OrthoDB; EOG7M98RQ; -. DR BioCyc; YEAST:G3O-31881-MONOMER; -. DR EvolutionaryTrace; P36075; -. DR NextBio; 974873; -. DR PRO; PR:P36075; -. DR Proteomes; UP000002311; Chromosome XI. DR GO; GO:0043130; F:ubiquitin binding; IDA:SGD. DR InterPro; IPR003892; CUE. DR InterPro; IPR002625; Smr_dom. DR InterPro; IPR009060; UBA-like. DR Pfam; PF02845; CUE; 2. DR SMART; SM00546; CUE; 2. DR SMART; SM00463; SMR; 1. DR SUPFAM; SSF160443; SSF160443; 1. DR SUPFAM; SSF46934; SSF46934; 2. DR PROSITE; PS51140; CUE; 2. DR PROSITE; PS50828; SMR; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Reference proteome; Repeat; KW Ubl conjugation pathway. FT CHAIN 1 443 Ubiquitin-binding protein CUE2. FT /FTId=PRO_0000079558. FT DOMAIN 8 51 CUE 1. {ECO:0000255|PROSITE- FT ProRule:PRU00468}. FT DOMAIN 55 98 CUE 2. {ECO:0000255|PROSITE- FT ProRule:PRU00468}. FT DOMAIN 347 443 Smr. {ECO:0000255|PROSITE- FT ProRule:PRU00321}. FT HELIX 9 19 {ECO:0000244|PDB:1OTR}. FT STRAND 21 23 {ECO:0000244|PDB:1OTR}. FT HELIX 25 34 {ECO:0000244|PDB:1OTR}. FT TURN 35 37 {ECO:0000244|PDB:1OTR}. FT HELIX 40 49 {ECO:0000244|PDB:1OTR}. SQ SEQUENCE 443 AA; 50874 MW; 8B7BB872C2BDC734 CRC64; MSMDNNDDHE SKLSILMDMF PAISKSKLQV HLLENNNDLD LTIGLLLKEN DDKSTVDNEL HQLYDMFPQL DCSVIKDQFV INEKSVESTI SDLLNYETLQ KLKDNQANSP DSVKRNEKKN NWESTNDHIE SIIKFTDAPK NIAQEYLAEN GFDTVKAIIK IILDYYDKRD FKKDVDTFKV KRSPNTTVRG GRVQSSTGLA HVLKKGKESA NVAQESLKRP RSYKHSLDSP QMVELNELVA DNRDLKAINH EFLQKCLQFY DGDVVKVLNI SSLLIEDDKN ITKTWNFDEG FTLTSRDNCK QHLPKFSTPQ ISRRNEVGNT YKLPLHDKET PEGAVPVINN LFQTYRLDFH GFLPSEAVST LKLALNKWWS KEVAERELNS HNINSYGSKV QFVSPLIVVT GRGIHSIGGI SKVRLQVKSF LEKNHYIFWE ESSYFRIEGK KKK //